Value of nitrogen oxide and cachectine in the pathogenesis of preterm birth
Objective. To investigate whether the impaired production of uterine contractility predictors in the serum from a pregnant woman plays a role in the pathogenesis of preterm birth.Drukker N.A., Selyutina S.N., Ermolova N.V., Chikin A.L., Palieva N.V.
Subjects and methods. A total of 134 pregnant women were followed up. Group 1 included 45 women with early preterm birth (PB) at 28–32 weeks and 6 days of gestation; Group 2 consisted of 42 women with PB at 34–36 weeks and 6 days of gestation, and a control group comprised 47 women with normal pregnancy and childbirth. Serum served as the material to be examined. The content of lipocortin (annexin) and the activity of nuclear NF-κB and phospholipase A2 were determined by enzyme immunoassay using kits (Cytimmune systems, USA); VEGFR-A was measured by kits (Red systems, USA). Nitric oxide (NO) levels were estimated using the classical Griess reaction after enzymatic reduction of nitrates to nitrites (designated as NOx in the literature) on a Varian Cary-50 spectrophotometer (USA) at a wavelength of 520 nm and was expressed in pkg/ml. The activity of NO synthase (NOS) (EC 1.14.13.39) was assessed by the level of metabolites from L-arginine.
Results. The production of cachectine and NO was found to be impaired, which led to elevated intracellular Ca2+ levels and caused a lower myometrial contraction. The lower activity of NOS was established to be due to hypoxia during early PB at 28–33 weeks and 6 days and 34-36 weeks and 6 days of gestation. In case of PB at 34–36 weeks and 6 days of gestation, there is an additional factor in reducing the activity of this enzyme, which is a decrease in its amount (synthesis).
Conclusion. The findings suggest that the production of cachectine and NO is modified in early and preterm births at 28–33 and 34–36 weeks, respectively. The results of blood cachectine levels in a pregnant woman at different gestational ages make it possible to predict PB in term of its pathogenesis.
Keywords
uterine contraction bioregulators
preterm birth
endothelial factors
serum
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Received 26.11.2019
Accepted 29.11.2019
About the Authors
Nina A. Drukker, Doctor of Biological Sciences, Associate Professor, Chief Researcher of the Department of Biomedical Problems in Obstetrics, Gynecology and Pediatrics FSBEI HE Rostov State Medical University of the Ministry of Health of Russia. Tel.: +7 (863)227-50-77. E-mail: n.drukker@yandex.ru. https://orcid.org/0000-0002-1605-6354.344012 Russia, Rostov-on-Don, GSP-704, ul. Mechnikov, d. 43.
Svetlana N. Selyutina, Candidate of Biological Sciences, Researcher, Department of Biomedical Problems in Obstetrics, Gynecology and Pediatrics,
FSBEI HE Rostov SMU of the Ministry of Health of the Russian Federation. Phone: + 7 (863)2275077. E-mail: edu.centr@rniiap.ru; https://orcid.org/0000-0003-2049-5592
344018, Rostov-on-Don, GSP-704, ul. Mechnikova, 43.
Natalia V. Ermolova, Doctor of Medical Sciences, Associate Professor of the Department of Obstetrics and Gynecology of Rostov State Medical University. Head of the Obstetric and Gynecological Department of the Federal State Budget Educational Establishment of Rostov State Medical University,
Phone: + 7 (863)2275065; https://orcid.org/0000-0002-4162-5934
344012, Rostov-on-Don, GSP-704, ul. Mechnikov, 43.
Aleksey L. Chikin, Doctor of Physics and Mathematics, Chief Researcher at the Federal Research Center Southern Scientific Center of the Russian Academy of Sciences. Phone: + 7 (904) 503-90-84. 344006 Russia, Rostov-on-Don, 41 Chekhov Ave.
Natalia V. Palieva, MD, associate professor, Chief Researcher, Rostov State Medical University of the Ministry of Health of Russia.
Phone: + 7 (863)3200268; https://orcid.org/0000-0003-2278-5198
344012, Rostov-on-Don, GSP-704, ul. Mechnikov, 43.
For citation: Drukker N.A., Selyutina S.N., Ermolova N.V., Chikin A.L., Palieva N.V. Value of nitrogen oxide and cachectine in the pathogenesis of preterm birth.
Akusherstvo i Ginekologiya/ Obstetrics and gynecology. 2020; 3: 72-8. (In Russian).
https://dx.doi.org/10.18565/aig.2020.3.72-78
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