The cluster approach to assessment of the subgingival microbiome composition in women with adverse pregnancy outcomes
Burduli A.G, Balmasova I.P., Tetruashvili N.K., Donnikov A.E., Krasheninnikova R.V., Arutyunov S.D.
Objective: To develop optimal approach to assessment of the clinical features of the gestational process and the role of associations of the members of the subgingival microbiome in the development of pregnancy complications associated with inflammatory changes in periodontium.
Materials and methods: The total number of patients in the study was 146 pregnant women, who were divided into 4 groups depending on the presence or absence of the concomitant inflammation of periodontal tissues (gingivitis) and/or local inflammation of the lower reproductive tract. The study design included obstetric and gynecological anamneses collection, assessment of the current pregnancy complications, determination of the composition of the subgingival microbiome and the vaginal microbiome using PCR. Statistical data processing included cluster analysis.
Results: The study found relationship between systemic manifestations of periodontal diseases and preterm birth, low birth weight. There was statistically significant relationship between these complications and gingivitis. Cluster analysis was used to identify 4 different associations of the microorganisms exhibiting periodontopathogenic properties, 3 of them were found in more than half of cases in the groups with concomitant gingivitis.
Conclusion: The cluster approach to assessment of the subgingival microbiome in pregnant women helped to identify associations of the microorganisms exhibiting periodontopathogenic properties. High frequency of their occurrence was accompanied by the presence of clinical signs of gingivitis and the development of systemic effects of pregnancy complications.
Authors' contributions: Burduli A.G., Balmasova I.P., Tetruashvili N.K. – the concept and design of the study; Burduli A.G., Donnikov A.E., Krasheninnikova R.V. – material collection and processing; Burduli A.G., Balmasova I.P. – data analysis; Burduli A.G. – article writing; Tetruashvili N.K., Donnikov A.E., Arutyunov S.D. – article editing.
Conflicts of interest: The authors confirm that they have no conflicts of interest to declare.
Funding: The study was conducted without any sponsorship.
Ethical Approval: The study was approved by the local Ethics Committee of the Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
Patient Consent for Publication: The patients have signed informed consent for publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Burduli A.G., Balmasova I.P., Tetruashvili N.K., Donnikov A.E., Krasheninnikova R.V., Arutyunov S.D.
The cluster approach to assessment of the subgingival microbiome composition in women with adverse pregnancy outcomes.
Akusherstvo i Gynecologia/Obstetrics and Gynecology. 2025; (9): 89-97 (in Russian)
https://dx.doi.org/10.18565/aig.2025.185
Keywords
pregnancy complications
preterm birth
fetal growth restriction
gingivitis
local inflammation of the lower reproductive tract
microbiome
cluster analysis
associations of periodontopathogenic microorganisms
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Received 03.07.2025
Accepted 22.08.2025
About the Authors
Anna G. Burduli, PhD, Senior Researcher at the B.V. Leonov Department of Assisted Technologies for the Treatment of Infertility, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, +7(495)531-44-44, +7(985)120-36-14, burdulianna@gmail.com, https://orcid.org/0000-0002-2849-5426Irina P. Balmasova, Dr. Med. Sci., Professor, Leading Researcher at the Laboratory of Molecular Biological Research, Scientific Research Medical Dentistry Institution, Scientific and Educational Institution of Basic Medicine named after V.I. Pokrovsky, Russian University of Medicine, Ministry of Health of Russia, 127006, Russia, Moscow, Dolgorukovskaya str. 4, +7(910)468-97-72, iri.balm@mail.ru, https://orcid.org/0000-0001-8194-2419
Nana K. Tetruashvili, Dr. Med. Sci., Associate Professor, Head of the Obstetric Department of Pregnancy Pathology No. 2, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, +7(495)438-14-77,
n_tetruashvili@oparina4.ru, https://orcid.org/0000-0002-9201-2281
Andrey E. Donnikov, PhD, Head of the Laboratory of Molecular Genetic Methods, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparina str., 4, +7(495)438-49-51, a_donnikov@oparina4.ru, https://orcid.org/0000-0003-3504-2406
Regina V. Krasheninnikova, Geneticist at the Laboratory of Molecular Genetic Methods, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Oparina str., 4, +7(495)438-49-51, ex. 3101, r_krasheninnikova@oparina4.ru,
https://orcid.org/0009-0002-2903-1225
Sergey D. Arutyunov, Dr. Med. Sci., Professor, Head of Department of Orthopedic Dentistry and Digital Technologies, Russian University of Medicine, Ministry of Health of Russia, 127473, Russia, Moscow, Delegatskaya str., 20-1, sd.arutyunov@mail.ru, https://orcid.org/0000-0001-6512-8724
Corresponding author: Anna G. Burduli, burdulianna@gmail.com
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