The role of peripheral blood platelet-monocyte aggregates in reproductive processes and their study methods

DOI

https://dx.doi.org/10.18565/aig.2021.8.50-58

Selyutin A.V., Chepanov S.V., Pavlov O.V., Kornyushina E.A., Selkov S.A.
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, Saint Petersburg, Russian Federation

This review analyzes and generalizes the results of the studies characterizing the interaction of peripheral blood platelets and monocytes, which have been conducted to define their role in various physiological and pathophysiological processes. At the same time, special attention was given to reproductively significant processes. Due to the fact that the magnitude, direction, and consequences of the interactions between these types of cells are most strongly characterized by the number, immunomorphological features, and functional activity of platelet-monocyte aggregates (PMAs) resulting from such interactions; particular emphasis was placed on the findings of the investigation of these structural formations. In particular, the review considers the results of studying the mechanisms of interaction between platelets and monocytes, which lead not only to the formation, but also to the stabilization of these structures. It describes the membrane-bound molecules and molecular complexes that provide the formation of PMAs, as well as soluble factors that favor the development of complexation mechanisms at the autocrine, juxtacrine, and paracrine regulation levels. The review contains the characteristics of methods for the isolation and identification of PMAs, such as differential and gradient centrifugation, immunomagnetic separation, electron microscopy, light and fluorescence microscopy, flow cytometry and the latter with visualization. Their advantages and disadvantages are analyzed from the point of view of adequacy, technological effectiveness, and clinical and diagnostic information value of the conducted studies. The data available in the literature on the role of PMAs in reproductive pathologies are assessed.
Conclusion. The contact interaction of platelets and monocytes plays an important role in various physiological and pathophysiological processes. The ability to effectively isolate and identify PMAs using modern technologies has created prerequisites for the use of measuring the concentration of PMAs as a diagnostic and prognostic indicator for diseases accompanied by hemostatic disorders. From the point of view of reproductive processes, the study of the effect of PMAs on endometrial and trophoblast cells is of absolute interest. An approach based on the creation of cell models using cell lines of the corresponding origin seems promising.

platelet

monocyte

trophoblast

placenta

platelet-monocyte aggregate

P-selectin

flow cytometry

Full text (in Russian)

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Received 05.02.2021

Accepted 27.04.2021

Alexander V. Selutin, Ph.D. (biol. sci.), Senior Researcher at the Immunology Laboratory with AIDS Diagnostics Group, Department of Immunology and Intercellular Interactions, D.O. Ott Research Institute for Obstetrics and Gynecology. Tel.: +7(812)328-98-50. Email: a_selutin@yahoo.com.
3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation.
Sergey V. Chepanov, Ph.D., Senior Researcher at the Proteomic Immunoregulation Group, Department of Immunology and Intercellular Interactions, D.O. Ott Research Institute for Obstetrics and Gynecology. Tel.: +7(812)328-98-50. E-mail: chepanovsv@gmail.com. 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation.
Oleg V. Pavlov, PhD, MD (biol. sci.), Leading Researcher at the Department of Immunology and Intercellular Interactions, Institute of Obstetrics, D.O. Ott Research Institute for Obstetrics and Gynecology. Tel.: +7(812) 328-98-50. E-mail: ovpavlov@hotmail.com. 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation.
Ekaterina A. Kornyushina, obstetrician-gynecologist at the Obstetric Department of Pathology of Pregnancy, Senior Researcher, D.O. Ott Research Institute for Obstetrics and Gynecology. E-mail: hapacheva@yandex.ru. 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation.
Sergey A. Selkov, Merited Scholar of the Russian Federation, Dr. Med. Sci., Professor, Head of the Department of Immunology and Intercellular Interactions, D.O. Ott Institute of Obstetrics, Gynecology and Reproductology. Tel.: +7(812)328-98-50. E-mail: selkovsa@mail.ru. 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation.

For citation: Selyutin A.V., Chepanov S.V., Pavlov O.V., Kornyushina E.A., Selkov S.A. The role of peripheral blood platelet-monocyte aggregates in reproductive processes and their study methods.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2021; 8: 50-58 (in Russian)
https://dx.doi.org/10.18565/aig.2021.8.50-58

The role of peripheral blood platelet-monocyte aggregates in reproductive processes and their study methods

Selyutin A.V., Chepanov S.V., Pavlov O.V., Kornyushina E.A., Selkov S.A.

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