Oxidative stress indicators in pregnant women, those who are healthy and those who have urogenital infection, in the early stages of gestation
Objective. To investigate oxidative stress indicators in women with early physiological pregnancy and in those with early pregnancy complicated by urogenital infection.Zhambalova B.A., Nosikova I.N., Malushenko S.V., Maksina A.G., Gankovskaya L.V., Dobrokhotova Yu.E., Osipov A.N., Tesyolkin Yu.O.
Subjects and methods. Examinations were made in 90 women aged 20-45 years, who were divided into 3 groups: 1) 30 healthy non-pregnant women; 2) 30 healthy pregnant women; 3) 30 pregnant women with urogenital infection. All the pregnant women were in the first trimester of pregnancy (at 8-10 weeks). The functional activity of whole blood phagocytes was determined by luminol-dependent chemiluminescence. A chemiluminescent method was also used to measure the antioxidant activity of plasma. The plasma content of one of the lipid peroxidation products, malondialdehyde, was determined spectrophotometrically.
Results. There was significantly enhanced functional activity of whole blood phagocytes in the healthy pregnant women and in the pregnant women with urogenital infection versus the healthy non-pregnant women, elevated malondialdehyde levels in their plasma, and its decreased antioxidant activity.
Conclusion. Comparison of the indicators of oxidative stress in pregnant women, those who are healthy and those who have urogenital infection, and in the healthy non-pregnant women leads to the conclusion that oxidative stress develops just in the first trimester of pregnancy. Urogenital infection in pregnant women contributes to an even greater imbalance between the production of prooxidants and the activity of the antioxidant system.
Keywords
physiological pregnancy
urogenital infection
blood phagocytes
reactive oxygen species
oxidative stress
plasma antioxidant activity
chemiluminescence
Supplementary Materials
- Fig.1. Intensity of luminol-dependent chemiluminescence of whole blood phagocytes (a) and normalized luminol-dependent chemiluminescence of whole blood phagocytes (b), stimulated by zymosan and opsonized zymosan.
- Fig. 2. Levelsof blood plasma malondialdehyde (а) and the total antioxidant capacity of blood plasma (b).
- Table. Oxidative stress index in women surveyed groups
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Received 23.11.2016
Accepted 23.12.2016
About the Authors
Zhambalova Bayarma Arsalanovna, Ph.D., Department of Medical Biophysics, N.I. Pirogov Russian National Research Medical University.117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +79854133427. E-mail: ZhambalovaBA@inbox.ru
Nosikova Inna Nikolaevna, student of N.I. Pirogov Russian National Research Medical University.
117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +79055806672. Е-mail: nosikovainna@mail.ru
Malushenko Svetlana Vasilevna, postgraduate of N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +74954941253. Е-mail: malusvetlana@yandex.ru
Maksina Alexandra Genrikhovna, Doctor of Biological Sciences, Professor, Department of Physics and Mathematics, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +74954348619. E-mail: maxinaalexandra@yandex.ru
Gankovskaya Lyudmila Viktorovna, Doctor of Medical Sciences, Professor, Department of Immunology, N.I. Pirogov Russian National Research Medical University.
117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +74954349000. Е-mail: lvgan@yandex.ru
Dobrokhotova Yuliya Eduardovna, Doctor of Medical Sciences, Professor, Department of Obstetrics and Gynecology, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +74952374033. E-mail: pr.dobrohotova@mail.ru
Osipov Anatoliy Nikolaevich, Doctor of Biological Sciences, Professor, Department of Medical Biophysics, N.I. Pirogov Russian National Research Medical University.
117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +74954342266, ext. 1410. E-mail: anosipov@yahoo.com
Teselkin Yuriy Olegovich – Doctor of Biological Sciences, Professor, Department of Medical Biophysics N.I. Pirogov Russian National Research Medical University.
117997, Russia, Moscow, Ostrovityanov str 1. Tel.: +74954342266, ext. 1411. E-mail: teselkin-box@mail.ru
For citations: Zhambalova B.A., Nosikova I.N., Malushenko S.V., Maksina A.G.,
Gankovskaya L.V., Dobrokhotova Yu.E., Osipov A.N., Tesyolkin Yu.O. Oxidative stress indicators in pregnant women, those who are healthy and those who have urogenital infection,
in the early stages of gestation. Akusherstvo i Ginekologiya/
Obstetrics and Gynecology. 2017; (9): 40-6. (in Russian)
http://dx.doi.org/10.18565/aig.2017.9.40-6
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