The features of the E-cadherin/β-catenin signaling pathway in the placenta and peripheral blood of pregnant women with fetal growth restriction
Krasnyi A.M., Khachaturyan A.A., Tyutyunnik V.L., Sorokina L.E., Kan N.E., Borisova A.G., Krasnova L.D., Sorivko E.R.
It is known that reduced placental size, abnormal development of placental villi and reduced E-cadherin expression are observed in pregnancies with fetal growth restriction.
Objective: To identify the features of the E-cadherin/β-catenin signaling pathway in the placenta and peripheral blood of pregnant women by determining expression of the MMP-9, CCND1 and BIRC5 genes induced by the nuclear β-catenin translocation, as well as WNT2 gene expression, and to analyze the features of apoptosis of the placental cells.
Materials and methods: The study included 82 patients. The main group consisted of 46 women with the postnatal diagnosis of fetal growth restriction without hypertension complications. The control group consisted of 36 patients with normal pregnancy. Investigation of the features of the E-cadherin/β-catenin signaling pathway was carried out by determining expression of the MMP-9, CCND1 and BIRC5 genes induced by the nuclear β-catenin translocation, and WNT2 gene expression in the placental tissue and peripheral blood, as well as analyzing the features of apptosis of placental cells.
Results: It was found that MMP-9 and BIRC5 expression levels in the placenta were significantly higher in fetal growth restriction (p=0.03 and p=0.02, respectively). MMP-9 expression level in the peripheral blood was lower in fetal growth restriction (p=0.001). Increased apoptosis of the placental cells was observed in fetal growth restriction (p=0.03). Excessive apoptosis of trophoblast cells was in fetal growth restriction compared with normal pregnancy (p=0.017).
Conclusion: Lower level of E-cadherin expression in the placenta in fetal growth restriction can be associated with activation of the E-cadherin/β-catenin signaling pathway and development of apoptosis of placental trophoblast cells.
Authors' contributions: Krasnyi A.M., Khachaturyan A.A., Tyutyunnik V.L., Sorokina L.E., Kan N.E., Borisova A.G., Krasnova L.D., Sorivko E.R. – the concept and design of the study, data obtaining for analysis, literature review, processing and analysis of material on the topic of the study, manuscript writing, article editing.
Conflicts of interest: The authors confirm that they have no conflicts of interest to declare.
Funding: The study was conducted without any sponsorship.
Ethical Approval: The study was approved by the local Ethics Committee of the Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health Russia.
Patient Consent for Publication: The patients have signed informed consent for publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Krasnyi A.M., Khachaturyan A.A., Tyutyunnik V.L., Sorokina L.E., Kan N.E., Borisova A.G.,
Krasnova L.D., Sorivko E.R. The features of the E-cadherin/β-catenin signaling pathway in the
placenta and peripheral blood of pregnant women with fetal growth restriction.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2025; (5): 49-56 (in Russian)
https://dx.doi.org/10.18565/aig.2025.43
Keywords
fetal growth restriction
placenta
Wnt
β-catenin
E-cadherin
apoptosis
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Received 24.02.2025
Accepted 17.04.2025
About the Authors
Aleksey M. Krasnyi, PhD, Head of the Cytology Laboratory, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology,Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, alexred@list.ru, https://orcid.org/0000-0001-7883-2702
Anuta A. Khachaturyan, PhD student, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, x.anyt37@mail.ru, https://orcid.org/0009-0007-3767-9343
Victor L. Tyutyunnik, Professor, Dr. Med. Sci., Leading Researcher at the Center of Scientific and Clinical Researches, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, tioutiounnik@mail.ru,
Researcher ID: B-2364-2015, SPIN: 1963-1359, Authors ID: 213217, Scopus Author ID: 56190621500, https://orcid.org/0000-0002-5830-5099
Leia E. Sorokina, Junior Researcher at the Cytology Laboratory, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, leya.sorokina@mail.ru, https://orcid.org/0000-0002-1862-6816
Natalia E. Kan, Professor, Dr. Med. Sci., Honored Scientist of the Russian Federation, Deputy Director of Science, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, kan-med@mail.ru, Researcher ID: B-2370-2015, SPIN: 5378-8437, Authors ID: 624900, Scopus Author ID: 57008835600, https://orcid.org/0000-0001-5087-5946
Anastasia G. Borisova, PhD student, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4 +7(968)735-40-81, vvv92@list.ru, https://orcid.org/0009-0004-5234-1584
Lidia D. Krasnova, 5th year student of the Institute of Clinical Medicine, majoring in General Medicine, N.I. Pirogov Russian National Research Medical University,
Ministry of Health of Russia, 117513, Russia, Moscow, Ostrovityanova str., 1, bld. 6, li.kr.2402@gmail.com, https://orcid.org/0009-0009-2718-3672
Evgeny R. Sorivko, obstetrician-gynecologist, Melitopol Regional Perinatal Center, 272301, Russia, Melitopol, Kiziyarskaya str., 37, awwgxtf@gmail.com
Corresponding author: Aleksey M. Krasnyi, alexred@list.ru
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