Specific features of a metabolomic profile in preeclampsia
Objective. To search for markers for predicting preeclampsia on the basis of metabolomic analysis using mass spectrometry and bioinformatics. Subjects and methods. The investigation included two groups of pregnant women: a study group of 13 pregnant women with preeclampsia and a comparison group of 21 pregnant women with physiological pregnancy. Serum metabolite profiles were obtained using liquid chromatography- mass spectrometry. Results. The investigators identified 22 amino acids with statistically significantly different concentrations: 3-methylhistidine, alanine, anserine, arginine, aspartate, carnosine, cystine, ethanolamine, glutamate, glutamine, glycine, histidine, homocitrulline, lysine, methionine, norvaline, O-phosphorylethanolamine, ornithine, phenylalanine, serine, threonine, and tyrosine. There was a moderate correlation with 3 amino acids: carnosine (KK = 0.526, p <0.001), arginine (KK = 0.437; p <0.001), ethanolamine (KK = 0.403; p = 0.003). In addition, there was a correlation of carnosine with hypertension (KK = 0.375; p = 0.038), proteinuria (KK = 0.543; p <0.001); arginine and ethanolamine also correlated with the level of high blood pressure (KK = 0.462; p = 0.009, and KK = 0.428; p = 0.002) and proteinuria (KK = 0.428; p = 0.002. and KK = 0.431, p = 0.002). Analysis of the ROC curves showed that carnosine, arginine, and ethanolamine were potentially sensitive and specific biomarkers for the diagnosis and prediction of preeclampsia (the area under the curve was 0.848, 0.789, and 0.767, respectively). Conclusion. The results of the investigation have shown that determination of the metabolic profile is of great clinical significance for predicting preeclampsia.Kan N.E., Lomova N.A., Amiraslanov E.Yu., Chagovets V.V., Tyutyunnik V.L., Khachatryan Z.V., Starodubtseva N.L., Kitsilovskaya N.A., Frankevich V.E.
Keywords
preeclampsia
metabolomics
prediction
biomarker
References
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Received 30.09.2019
Accepted 04.10.2019
About the Authors
Natalia E. Kan, PhD, MD, professor of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Tel.: +7-926-220-86-55. E-mail: kan-med@mail.ru. 117997 Russia, Moscow, Ac. Oparina, 4 str.Natalia A. Lomova, PhD, Researcher of the Institute of Obstetrics of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Tel.: +79161442162. E-mail: natasha-lomova@yandex.ru. 117997 Russia, Moscow, Ac. Oparina, 4 str.
Elrad Yu Amiraslanov., PhD, the head of the obstetric department of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Тел. +7-916-549-20-94 E-mail: e_amiraslanov@oparina4.ru
117997 Russia, Moscow, Ac. Oparina, 4 str.
Vitaliy V. Chagovets, PhD, Senior Researcher of Laboratory of Proteomics and Metabolomics of Human Reproduction of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation.
Tel.: +79169191466. E-mail: vvchagovets@gmail.com 117997 Russia, Moscow, Ac. Oparina, 4 str.
Victor L. Tyutyunnik, PhD, MD, professor of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Tel.: +7-903-969-50-41. E-mail: tioutiounnik@mail.ru. Number Researcher ID B-2364-2015.ORCID ID 0000-0002-5830-5099
117997 Russia, Moscow, Ac. Oparina, 4 str.
Zarine V. Khachatryan, PhD student of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Tel.: +79096562456. E-mail: z.v.khachatryan@gmail.com. 117997 Russia, Moscow, Ac. Oparina, 4 str.
Nataliia L Starodubtseva., PhD, Head of Laboratory of Proteomics of Human Reproduction, National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Tel.: +79164639867. e-mail: n_starodubtseva@oparina4.ru
117997 Russia, Moscow, Ac. Oparina, 4 str.
Natalia A. Kitsilovskaya, specialist of the Laboratory of Proteomics and Metabolomics in Human Reproduction, Department of Systems Biology in Reproduction of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation.
Tel. +79035657139 E-mail: kitsilovskyana@gmail.com. 117997 Russia, Moscow, Ac. Oparina, 4 str.
Vladimir E. Frankevich, PhD, Head of Department of Systems Biology in Reproduction of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation. Tel.: +74954380788, ext. 2198. E-mail: v_frankevich@oparina4.ru
117997 Russia, Moscow, Ac. Oparina, 4 str.
For citation: Kan N.E., Lomova N.A., Amiraslanov E.Yu., Chagovets V.V., Tyutyunnik V.L., Z.V. Khachatryan, N.L. Starodubtseva, N.A. Kitsilovskaya, V.E. Frankevich. Specific features of a metabolomic profile in preeclampsia
Akusherstvo i Ginekologiya / Obstetrics and Gynecology. 2019; (11): 82-8.(in Russian).
http://dx.doi.org/10.18565/aig.2019.11.82-88
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