Characteristics of coagulase-negative Staphylococci isolated from central venous catheters of neonates in an intensive care unit: formation of biofilm and genetic population structure identified by multilocus sequencing typing
Aim. To investigate the ability of hospital-associated populations of S. epidermidis to form biofilms on the inner surface of central venous catheters (CVC).Kornienko M.A., Kuptsov N.S., Gorodnichev R.B., Malakhova M.V., Lyubasovskaya L.A., Gordeev A.B., Nikitina I.V., Smirnov G.B., Shitikov E.A., Priputnevich T.V., Ilyina E.N.
Material and methods. The study analyzed 150 samples of CVCs obtained after catheterization of neonates.
Results. Fifty three catheters were colonized by conditional pathogens, and 50 samples were contaminated by a single strain. Isolates of S. epidermidis were present in 68% of samples. They were assigned to 12 different sequence types, most frequently to ST59 (19 isolates, 56%). The icaADBC operon genes, which are involved in the production of the polysaccharide intercellular adhesin (PIA), were not detected in the majority of isolates.
Conclusion. Most of CVCs were colonized by S. epidermidis (62% of isolates) with sequence types belonging to the CC2 clonal complex, thus indicating that they are hospital acquired. A group of isolates with an increased ability to adhere to plastics was identified. Some isolates were found to be ica-operon negative, but capable of forming a biofilm on the catheter surface by an ica-independent mechanism.
Keywords
coagulase-negative staphylococci
biofilms
catheter-associated infections
newborn children
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Received 08.09.2017
Accepted 22.09.2017
About the Authors
Kornienko Maria A., PhD, Junior researcher in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia. 119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451. E-mail: kornienkomariya@gmail.comKuptsov Nikita S., assistant in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine,
Federal Biomedical Agency of Russia. 119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451
Gorodnichev Roman B., Junior researcher in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia. 119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451
Malakhova Maya V., Researcher in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine,
Federal Biomedical Agency of Russia. 119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451
Lyubasovskaya Lyudmila A., PhD, Head of the Unit of clinical pharmacology, Department of microbiology and clinical pharmacology, National Medical Research Center
for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Healthcare of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79060744246. E-mail: labmik@yandex.ru
Gordeev Aleksey B., Senior Researcher in the Laboratory of microbiology, Department of microbiology and clinical pharmacology, National Medical Research Center
for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Healthcare of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79162268667. E-mail: gordeew@vega.protres.ru
Nikitina Irina V., Senior Researcher in the Unit of resuscitation and intensive care of newborns, Department of neonatology and pediatrics,
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Healthcare of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382271. E-mail: i_nikitina@oparina4.ru
Smirnov Georgy B., Chief Scientific Officer in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia. 119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451
Shitikov Egor A., Senior researcher in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia. 119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451
Priputnevich Tatyana V., Head of the Department of microbiology and clinical pharmacology, National Medical Research Center for Obstetrics,
Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Healthcare of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79104145616. E-mail: priput1@gmail.com
Ilyina Elena N., Deputy director for science, Federal Research and Clinical Center of Physical-chemical medicine of Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia.
119435, Russia, Moscow, Malaya Pirogovskaya 1a. Tel.: +79037782451
For citations: Kornienko M.A., Kuptsov N.S., Gorodnichev R.B., Malakhova M.V., Lyubasovskaya L.A., Gordeev A.B., Nikitina I.V., Smirnov G.B., Shitikov E.A.,
Priputnevich T.V., Ilyina E.N. Characteristics of coagulase-negative Staphylococci isolated from central venous catheters of neonates in an intensive care unit: formation of biofilm and genetic population structure identified by multilocus sequencing typing.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (5): 86-94. (in Russian)
https://dx.doi.org/10.18565/aig.2018.5.86-94
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