Characteristic effect of prednisolone in vitro administered in the form of prednisolone sodium phosphate in a combined preparation on biofilm-forming microorganisms of the vaginal biotope
Khusnutdinova T.A., Spasibova E.V., Shalepo K.V., Budilovskaya O.V., Krysanova A.A., Cheberya A.S., Cheberya A.R., Savicheva A.M.
Objective: To evaluate the effect of prednisolone in vitro administered in the form of prednisolone sodium phosphate in the Elzhina combined preparation on biofilm-forming microorganisms of the vaginal biotope.
Materials and methods: The study included clinical isolates forming the films of the following bacteria: Klebsiella pneumoniae (3), Escherichia coli (2), Enterococcus faecalis (3), Streptococcus agalactiae (1), Gardnerella vaginalis (8), Staphylococcus epidermidis (1), Candida albicans (5), Candida dubliniensis (1), Candida glabrata (1), Candida parapsilosis (1). There were 26 isolates. Dense and liquid selective nutrient media were used for cultivation, storage and further research. Microorganisms were identified using MALDI-TOF mass spectrometry (Bruker Microflex). The ability to form biofilms was assessed using a modified protocol of Christensen et al. (1985). The Elzhina preparation (solution of 1 tablet in 10 ml of physiological solution) and solutions of the main active ingredients of the preparation, i.e. neomycin, ornidazole, econazole, prednisolone (in the form of sodium phosphate), in concentrations corresponding to their content in the preparation were used to assess the effect on bacterial films.
Results: The Elzhina preparation showed a destructive effect on most biofilms that were formed by isolates of microorganisms included in the study (the coefficient of change of optical density after exposure to the Elzhina preparation was more than 1.0 in 96% of the isolates studied). The inclusion of prednisolone in the composition of the drug enhances the effect of the drug components in destroying biofilms formed by bacteria and yeast-like fungi. When determining the sensitivity of one isolate of K. pneumoniae to neomycin sulphate and four isolates of C. albicans to econazole in the presence of prednisolone, there was an increase in the zone of growth retardation around the inoculum with antibiotic towards the site of application of prednisolone sodium phosphate.
Conclusion: Prednisolone sodium phosphate, which has no direct antimicrobial and antifungal effect, has been shown to increase the antibacterial and antifungal effect of neomycin, ornidazole, econazole, contained in the Elzhina vaginal tablets, on bacteria and yeast-like fungi, as well as to increase the destructive effect of the preparation on bacterial films.
Authors’ contributions: All authors contributed substantially to the conception, research and preparation of the article; the final version was approved before publication. Khusnutdinova T.A. – literature review, analysis of the obtained data, writing the text; Shalepo K.V., Spasibova E.V. – conducting the study, analysis of the obtained data; Budilovskaya O.V., Krysanova A.A., Cheberya A.S., Cheberya A.R. – editing the text, approving the final version of the article; Savicheva A.M. – developing the concept and design of the study, analysis of the obtained data, final edition.
Conflicts of interest: Authors declare lack of the possible conflicts of interest.
Funding: The study was supported by Vertex JSC, Russia. The sponsor had no influence on the selection of material for publication, data analysis and interpretation.
Ethical Approval: The study was approved by the Ethical Review Board of the D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, protocol No. 134 as of April 16, 2024.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Khusnutdinova T.A., Spasibova E.V., Shalepo K.V., Budilovskaya O.V., Krysanova A.A., Cheberya A.S., Cheberya A.R., Savicheva A.M. Characteristic effect of prednisolone in vitro administered in the form of prednisolone sodium phosphate in a combined preparation on biofilm-forming microorganisms of the vaginal biotope.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2025; (3): 189-198 (in Russian)
https://dx.doi.org/10.18565/aig.2025.81
Keywords
biofilms
vaginal microorganisms
antimicrobial therapy
neomycin
ornidazole
econazole
prednisolone (in the form of prednisolone sodium phosphate)
Elzhina
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Received 20.03.2025
Accepted 27.03.2025
About the Authors
Tatiana A. Khusnutdinova, PhD, Senior Researcher of the Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, St. Petersburg, Mendeleevskaya Line, 3; Teaching Assistant at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, +7(981)725-96-90, husnutdinovat@yandex.ru, https://orcid.org/0000-0002-2742-2655Elena V. Spasibova, bacteriologist at the Laboratory of Clinical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleyevskaya Line, 3; Teaching Assistant at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, elena.graciosae@gmail.com, https://orcid.org/0009-0002-6070-4651
Kira V. Shalepo, PhD, Senior Researcher at the Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleevskaya Line, 3; Аssociate Professor at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, +7(911)247-41-51, 2474151@mail.ru, https://orcid.org/0000-0002-3002-3874
Olga V. Budilovskaya, PhD, Senior Researcher at the Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleevskaya Line, 3; Teaching Assistant of the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, +7(911)912-77-52, o.budilovskaya@gmail.com,
https://orcid.org/0000-0001-7673-6274
Anna A. Krysanova, PhD, Senior Researcher at the Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleevskaya Line, 3; Teaching Assistant at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, +7(911)737-96-93, krusanova.anna@mail.ru, https://orcid.org/0000-0003-4798-1881
Alexandra S. Cheberya, laboratory assistant researcher at Microbiology Laboratory, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleevskaya Line, 3, alexa-vorobjeva.09@yandex.ru, https://orcid.org/0009-0008-1091-5753
Alexander R. Cheberya, laboratory assistant researcher at Microbiology Laboratory, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, St. Petersburg, Mendeleevskaya Line, 3, sanekcheberya@yandex.ru, https://orcid.org/0009-0006-9058-6720
Alevtina M. Savicheva, Dr. Med. Sci., Professor, Head of the Department of Medical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, St. Petersburg, Mendeleevskaya Line, 3; Head of the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University,
Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, +7(921)944-15-47, savitcheva@mail.ru, https://orcid.org/0000-0003-3870-5930
Corresponding author: Tatiana A. Khusnutdinova, husnutdinovat@yandex.ru
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