Evaluation of the in vitro effectiveness of the Depantol components on biofilms produced by vaginal microorganisms
Shalepo K.V., Spasibova E.V., Budilovskaya O.V., Krysanova A.A., Khusnutdinova T.A., Cheberya A.S., Cheberya A.R., Savicheva A.M.
Objective: To evaluate the in vitro effectiveness of the Depantol components on biofilms produced by vaginal microorganisms.
Materials and methods: The study examined 33 clinical isolates of pure cultures of microorganisms obtained from the vaginal biotope. The bacterial films were produced by the following microorganisms: G. vaginalis (2 isolates), E. coli (3 isolates), K. pneumoniae (3 isolates), K. piersonii (1 isolate), C. freundii (3 isolates), A. baumannii (3 isolates), S. agalactiae (3 isolates), E. faecalis (3 isolates), S. aureus (3 isolates), and yeast-like fungi of Candida (9 isolates).
Dense and liquid selective nutrient media were used for the study. Microorganisms were identified using MALDI-TOF mass spectrometry (Bruker Microflex). The ability of microorganisms to produce biofilms was assessed according to a modified protocol of Christensen et al. (1985).
The in vitro effectiveness of the Depantol components on biofilms was evaluated using different dilutions of the drug components (chlorhexidine bigluconate, dexpanthenol, macrogols).
Results: The biofilms of varying density were formed by all 33 clinical isolates. Dexpanthenol in tested concentrations and macrogol had no effect on biofilms. Chlorhexidine bigluconate 1% solution destroyed biofilms produced by most vaginal bacteria. These are primarily G. vaginalis (both isolates tested), all E. coli, the clinical isolate of K. piersonii, all E. faecalis, S. aureus and all isolates of yeast-like fungi of Candida spp., both C. albicans and C. non-albicans (C. parapsilosis and C. glabrata). The biofilm was destroyed in two of the three isolates of K. pneumoniae, C. freundii, A. baumannii, and S. agalactiae.
Conclusion: Among the Depantol components, chlorhexidine bigluconate 1% solution was 100% effective on bacterial films produced by Gardnerella vaginalis, Escherichia coli, Klebsiella piersonii, Enterococcus faecalis, Staphylococcus aureus and yeast-like fungi of Candida spp., both C. albicans and C. non-albicans (C. parapsilosis and C. glabrata). Its effectiveness was 70% for the other microorganisms.
Authors’ contributions: Savicheva A.M. – review of the publications on the topic of the article, analysis of the obtained data, approval of the final version of the article; Shalepo K.V., Spasibova E.V. – conducting the study, writing the manuscript; Budilovskaya O.V., Khusnutdinova T.A., Krysanova A.A., Cheberya A.S., Cheberya A.R. – editing the text, approval of the final version of the article.
Conflicts of interest: Authors declare lack of the possible conflicts of interest.
Funding: The study was conducted without sponsorship.
Ethical Approval: The study was approved by the Ethical Review Board of the D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Shalepo K.V., Spasibova E.V., Budilovskaya O.V., Krysanova A.A., Khusnutdinova T.A., Cheberya A.S., Cheberya A.R., Savicheva A.M. Evaluation of the in vitro effectiveness
of the Depantol components on biofilms produced by vaginal microorganisms.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2024; (10): 158-166 (in Russian)
https://dx.doi.org/10.18565/aig.2024.256
Keywords
biofilms
vaginal microorganisms
chlorhexidine bigluconate
dexpanthenol
macrogol
Depantol
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Received 18.10.2024
Accepted 23.10.2024
About the Authors
Kira V. Shalepo, PhD, Senior Researcher at the Department of Experimental Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; Associate Professor at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, +7(911)247-41-51, 2474151@mail.ru, https://orcid.org/0000-0002-3002-3874
Elena V. Spasibova, bacteriologist at the Laboratory of Clinical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; Teaching Assistant at the Department of Clinical Laboratory Diagnostics of FP and DPO, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, elena.graciosae@gmail.com, https://orcid.org/0009-0002-6070-4651
Olga V. Budilovskaya, PhD, Senior Researcher at the Department of Medical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; Teaching Assistant at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, o.budilovskaya@gmail.com, https://orcid.org/ 0000-0001-7673-6274
Anna A. Krysanova, PhD, Senior Researcher at the Department of Experimental Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; Associate Professor at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, krusanova.anna@mail.ru, https://orcid.org/ 0000-0003-4798-1881
Tatiana A. Khusnutdinova, PhD, Senior Researcher at the Department of Medical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; Teaching Assistant at the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, husnutdinovat@yandex.ru, https://orcid.org/ 0000-0002-2742-2655
Alexandra S. Cheberya, laboratory assistant researcher at Microbiology Laboratory, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; 6th year student, S.M. Kirov Military Medical Academy, alexa-vorobjeva.09@yandex.ru,
https://orcid.org/0009-0008-1091-5753
Alexander R. Cheberya, laboratory assistant researcher at Microbiology Laboratory, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; 6th year student, S.M. Kirov Military Medical Academy, sanekcheberya@yandex.ru,
https://orcid.org/ 0009-0006-9058-6720
Alevtina M. Savicheva, Dr. Med. Sci., Professor, Head of the Department of Medical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia; Head of the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, savitcheva@mail.ru, https://orcid.org/ 0000-0003-3870-5930
Corresponding author: Kira V. Shalepo, 2474151@mail.ru
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