Experience of using vaginal autoprobiotics in a patient with spontaneous triplet pregnancy with recurrent vaginal anaerobic dysbiosis

Barinova V.V., Bushtyreva I.O., Kuznetsova N.B., Suvorov A.N., Botasheva T.L.

1) Rostov State Medical University, Ministry of Health of Russia, Rostov-on-Don, Russia; 2) Professor Bushtyreva Clinic, Rostov-on-Don, Russia; 3) Saint-Petersburg State University, Saint-Petersburg, Russia

Relevance: The incidence of preterm birth associated with premature rupture of the membranes remains at a stable level in the world despite the development of new approaches to therapy and their standardization. The rupture of the membranes during pregnancy is known to be directly associated with anaerobic vaginal dysbiosis. Multiple pregnancy in women with vaginal dysbiosis almost always ends in preterm delivery of infants with extremely and very low birth weight. The existing approaches to the treatment of vaginal dysbiosis during pregnancy do not always show a 100% effectiveness; therefore, it is necessary to find new alternative ways to normalize the vaginal microbiota. Case report: We present a clinical case of recurrent anaerobic vaginal dysbiosis in a pregnant woman with spontaneous triplet pregnancy who was prescribed vaginal autoprobiotics. The woman was examined and treated at Professor Bushtyreva Clinic, Rostov-on-Don, Russia. The prescription of vaginal autoprobiotics made it possible to normalize the state of the vaginal microbiota of the pregnant woman, obtain absolute normocenosis, avoid premature rupture of the membranes and deliver full-term infants in high-risk pregnancy. Conclusion: The prescription of vaginal autoprobiotics as therapy for anaerobic dysbiosis is a personalized and safe method of treatment, since autoprobiotics represent a woman’s own beneficial lactobacillus vaginal microflora that is genetically close and that does not cause rejection reactions. It is necessary to carry out further extensive studies on the effectiveness of vaginal autoprobiotics in pregnancy for the possible popularization of this technique.

Authors’ contributions: Barinova V.V., Bushtyreva I.O., Suvorov A.N. – developing the design and concept of the study; Kuznetsova N.B., Barinova V.V., Botasheva T.L. – collecting and processing the material, writing the text; Kuznetsova N.B., Suvorov A.N. – editing the article.

Conflicts of interest: The authors declare no possible conflicts of interest.

Funding: The study was conducted without sponsorship.

Patient Consent for Publication: The patient signed an informed consent for the publication of her data.

For citation: Barinova V.V., Bushtyreva I.O., Kuznetsova N.B., Suvorov A.N., Botasheva T.L. Experience of using vaginal autoprobiotics in a patient with spontaneous triplet pregnancy with recurrent vaginal anaerobic dysbiosis. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2023; (10): 184-192 (in Russian) https://dx.doi.org/10.18565/aig.2023.6


anaerobic dysbiosis
premature rupture of the membranes
preterm birth
multiple pregnancy


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Received 13.01.2023

Accepted 25.08.2023

About the Authors

Viktoriya V. Barinova, PhD, Assistant of the Department of Obstetrics and Gynecology No. 1, Rostov State Medical University, Ministry of Health of Russia, 344022, Russia, Rostov-on-Don, Nakhichevansky str., 29; Head of the Obstetric Department, Professor Bushtyreva Clinic, 344011, Russia, Rostov-on-Don, Soborniy str., 58/7,
+7(928)909-55-68, victoria-barinova@yandex.ru, https://orcid.org/0000-0002-8584-7096, WoS Researcher ID: https://publons.com/researcher/AAH-3314-2019,
Scopus AuthorID: https://www.scopus.com/authid/detail.uri?authorId=2578513
Natalya B. Kuznetsova, Dr. Med. Sci., Professor of the Simulation Training Center, Rostov State Medical University, Ministry of Health of Russia, 344022, Russia,
Rostov-on-Don, Nakhichevansky str., 29; Chief Physician, Professor Bushtyreva Clinic, 344011, Russia, Rostov-on-Don, Soborniy str., 58/7, +7(928)770-97-62,
Irina O. Bushtyreva, Dr. Med. Sci., Professor, Director, Professor Bushtyreva's Clinic, 344011, Russia, Rostov-on-Don, Sobornyi str., 58/7, +7(928)296-15-97, kio4@mail.ru.
Alexander N. Suvorov, Dr. Med. Sci., Professor, Corresponding Member of the Russian Academy of Sciences, Head of the Department of Molecular Microbiology, Institute of Experimental Medicine, 197022, Russia, St. Petersburg, Academician Pavlov str., 12, suvorov.an@ iemspb.ru
Tatyana L. Botasheva, Dr. Med. Sci., Professor, Chief Researcher of the Obstetrics and Gynecology Department, Research Institute of Obstetrics and Pediatrics, Rostov State Medical University, Ministry of Health of Russia, 344022, Russia, Rostov-on-Don, Nakhichevansky str., 29, t_botasheva@mail.ru, Scopus ID 55531205100, Web of Science ResearcherID: HKE-0537-2023

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