New approaches to studying the regulation of preimplantation embryonic development

Martirosyan Ya.O., Nazarenko T.A., Kadaeva A.I., Krasnova V.G., Biryukova A.M., Pogosyan M.T.

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia
Only 40% of embryos obtained in ART programs are known to develop to the blastocyst stage; the remaining embryos stop developing at different division stages. The genetic and epigenetic factors of both the very embryo itself and the oocyte, which play a key role in the early preimplantation embryonic development, are of great importance in the genesis of infertility.
This review discusses new approaches to studying the regulation of preimplantation embryo development, analyzes the impact of the genetic and epigenetic status of oocytes on the quality of obtained embryos and the rate of pregnancy. It gives the data of investigations in this area and promises of their use in clinical practice. On the strength of thee available data, the authors consider the possibility of overcoming severe infertility forms associated with a small number of obtained embryos and their poor quality; that of overcoming a condition, such as infertility of unclear genesis; they also discuss the possibility of searching for new methods for assessing the quality of oocytes and embryos to improve IVF programs. The review underlines the need for expanding and intensifying the investigations determining the epigenetic status of an embryo and the corresponding oocyte, those studying the processes of epigenetic regulation in the early embryo development.
The paper analyzes the data of researches in the epigenetic regulation of preimplantation embryonic development, characterizes the main epigenetic modifications affecting the quality of obtained oocytes and embryos, and identifies the possibility of clinical application of the available data.
Conclusion: At the present stage, the methods for assessing the quality of obtained embryos are based on morphological criteria. However, the morphological assessment grounded mainly on the visual determination of the degree of their competence does not correlate with the capability of embryos for implantation and development of high-quality pregnancy to a sufficient extent. In this connection, it is expedient to study the molecular genetic characteristics of embryos during the period of early embryonic development to devise clinical and pathogenetic methods to enhance the efficiency of infertility treatment in the IVF programs.

Authors' contributions: Martirosyan Ya.O., Nazarenko T.A., Kadaeva A.I., Krasnova V.G., Biryukova A.M., Pogosyan M.T. – development of the design of the investigation, obtaining the data to be analyzed, review of publications on the topic of the article, analyzing the findings, writing the text of the manuscript.
Conflicts of interest: The authors declare that there are no possible conflicts of interest.
Funding: The investigation was conducted within the state assignment 22-А21 "Solving the problem of infertility in modern conditions by developing a clinical diagnostic model of infertility".
For citation: Martirosyan Ya.O., Nazarenko T.A., Kadaeva A.I., Krasnova V.G., Biryukova A.M., Pogosyan M.T. New approaches to studying the regulation of
preimplantation embryonic development.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2023; (6): 29-37 (in Russian)


embryonic development stop
preimplantation embryonic development


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Received 16.01.2023

Accepted 22.06.2023

About the Authors

Yana O. Martirosyan, PhD, gynecologist at F. Paulsen Scientific and Clinical Department, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, +7(925)124-99-99,, 117997, Russia, Moscow, Ac. Oparina str., 4.
Tatiana A. Nazarenko, Professor, Dr. Med. Sci., Director of the Institute of Reproductive Medicine, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, +7(495)531-44-44,, 117997, Russia, Moscow, Ac. Oparina str., 4.
Albina I. Kadaeva, clinical resident specializing in obstetrics and gynecology, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, +7(917)762-82-11,, 117997, Russia, Moscow, Ac. Oparina str., 4.
Valeria G. Krasnova, clinical resident at F. Paulsen Scientific and Clinical Department, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, +7(495)531-44-44,, 117997, Russia, Moscow, Ac. Oparina str., 4.
Almina M. Biryukova, PhD, gynecologist at F. Paulsen Scientific and Clinical Department, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, +7(495)531-44-44,, 117997, Russia, Moscow, Ac. Oparina str., 4.
Mariam T. Pogosyan, Ph.D. student, F. Paulsen Scientific and Clinical Department, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, +7(495)531-44-44,, 117997, Russia, Moscow, Ac. Oparina str., 4.

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