A change in the concentration of extracellular DNA during pregnancy
Objective. To determine the plasma concentrations of total extracellular DNA (tDNA) and its fractions during pregnancy, by taking into account the possible impact of the body mass index (BMI) of women, anemia in pregnant women, fetal and placenta weight.Karapetyan A.O., Krasnyi A.M., Sadekova A.A., Khlestova G.V., Balashov I.S., Baev O.R.
Material and methods. The blood concentrations of tDNA, fetal DNA (fDNA) and maternal DNA (mDNA) were determined in women at 11–14, 24–26, and 30–32 weeks. The level of tDNA was estimated by quantitative PCR through determination of the concentration of the RASSF1A gene and that of fDNA was calculated through determination of the hypermethylated RASSF1A gene.
Results. The concentration of extracellular tDNA and mDNA did not change substantially during pregnancy. Until mid-gestation, there was a relatively stable fDNA level (averaged 1265.11±577.51 GE/ml at 11–14 weeks and 1309.09±561.01 GE/ml at 24–26 weeks) and its considerable increase in the second half of pregnancy. The level of extracellular DNA and its fractions did not change in relation to the women’s BMI and fetal and placental weight. In anemia, the concentration of mDNA averaged 12362.33±5533.22 GE/ml and was significantly lower than in healthy pregnant women (an average of 15268.49±5973.43) (p < 0.05).
Conclusion. The findings confirm the change in the concentration of total extracellular DNA during pregnancy, which must be taken into account when predicting its complications. To eliminate the effects of other maternal and fetal factors, it is necessary to continue investigations.
Keywords
total extracellular DNA
maternal extracellular DNA
fetal extracellular DNA
RASSF1A gene
placental dysfunction
pregnancy complications
anemia
Supplementary Materials
- Table 1. Sequences of primers and probes.
- Table 2. Clinical and anamnestic characteristics.
- Table 3. Concentration of mDNA and fDNA in uncomplicated pregnancy and anemia.
- Figure 1. Concentration of mDNA and fDNA during pregnancy (mDNA and weeks, fDNA and weeks).
- Figure 2. Concentration of mDNA and fDNA with considering the BMI (mDNA and BMI, fDNA and BMI).
- Figure 3. Concentration of mDNA and fDNA with considering the neonatal weight (mDNA and neonatal weight, fDNA and neonatal weight).
- Figure 4. Concentration of fDNA with considering the placental mass (fDNA and placental mass).
References
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Received 24.08.2017
Accepted 22.09.2017
About the Authors
Karapetyan Anna Ovikovna, post-graduate student, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79057068481. E-mail: anne-89@mail.ruKrasnyi Alexey Mikhailovich, Ph.D., head. Laboratory of Cytology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79637503535. E-mail: a_krasnyi@oparina4.ru
Khlestova Galina Vladimirovna, post-graduate student, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79647838076. E-mail: g_khlestova@oparina4.ru
Sadekova Alsu Amirovna, Researcher, Laboratory of Cytology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79267017386. E-mail: a_sadekova@oparina4.ru
Balashov Ivan Sergeevich, Junior Researcher, Laboratory of Bioinformatics, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79104462005. E-mail: i_balashov@oparina4.ru
Baev Oleg Radomirovich, MD, professor, head of the maternity department, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381188. E-mail: o_baev@oparina4.ru
For citations: Karapetyan A.O., Krasnyi A.M., Sadekova A.A., Khlestova G.V., Balashov I.S., Baev O.R. A change in the concentration of extracellular DNA during pregnancy.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (3): 44-50. (in Russian)
https://dx.doi.org/10.18565/aig.2018.3.44-50
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