Expression of vitamin d and its receptors in the villous chorion in missed miscarriage
Vitamin D receptors are known to be found in 36 human tissues, including in the ovarian tissue, endometrium, fallopian tubes, decidua, and placenta of women. In the early stages, trophoblast simultaneously produces and responds to vitamin D that has a local anti-inflammatory effect and induces decidual tissue growth for a successful pregnancy.Bespalova O.N., Bakleicheva M.O., Kovaleva I.V., Tolibova G.Kh., Tral T.G., Kogan I.Yu.
Objective. To investigate the expression of vitamin D and its receptor in the villous chorion in non-developing and physiological pregnancy.
Materials and methods. Sixty-four villous chorion samples divided into 2 groups: 1) 32 from women with missed miscarriage; 2) 32 from those who had undergone medical abortion, were examined. The inclusion criteria were abortion material from women at 6‒12 weeks’ gestation, singleton pregnancy, and normal fetal karyotype.
Results. The trophoblast and stroma of the chorionic villi showed the uniform distribution of the expression of vitamin D and its receptors. In missed miscarriage, the relative area of the expression of vitamin D and its receptor was statistically smaller than that in Group 2 (10.3%, 15.4% (p < 0.01) and 35.9±1.8; 56.1±2.4 (p < 0.01), respectively).
Conclusion. In missed miscarriage, the expression of vitamin D and its receptor is lower.
Keywords
expression of vitamin D
expression of vitamin D receptor
medical abortion
first-trimester spontaneous miscarriage
chorionic villi
immunohistochemistry
confocal microscopy
References
- Сидельникова В.М., Сухих Г.Т. Невынашивание беременности: руководство для практикующих врачей. М.; 2011. [Sidel’nikovaV.M., Suhih G.T. Nevynashivanie beremennosti: rukovodstvo dlja praktikujushhih vrachej. Moscow; 2011. (InRuss.)]
- Коган И.Ю., Гзгзян А.М., Лесик Е.А. Протоколы стимуляции яичников в циклах ЭКО, руководство для врачей. 2-е изд. М.; 2018. [Kogan I.Y., Gzgzyan A.M., Lesik E.A.Protokoly stimulyatsyi yaichnikov v tsyklah EKO, rukovodstvo dlya praktikuyuschih vrachey. 2-nd edn. Moscow; 2018.(InRuss.)].
- Palacios C., Gonzalez L. Is vitamin D deficiency a major global public health problem? J Steroid BiochemMol Biol. 2014;144(Pt A):138–45. doi: 10.1016/j.jsbmb.2013.11.003
- Прилепская В.Н., Короткова Н.А. Применение витаминно-минеральных комплексов при подготовке к зачатию, во время беременности и после родов. Эффективная фармакотерапия. 2013; 51: 24-29. [Prilepskaya V.N., Korotkova N.A. Use of vitamin-mineral complexes during preparation to conception, in pregnancy and after childbirth. Jeffektivnaja farmakoterapija. 2013; 51: 24–29. (InRuss.)]
- WHO recommendations on antenatal care for a positive pregnancy experience, 2016.
- Gonçalves D.R., Braga A., Braga J., Marinho A. Recurrent pregnancy loss and vitamin D: A review of the literature. Am J Reprod Immunol. 2018; 80(5): e13022. doi: 10.1111/aji.13022.
- Holmes V.A., Barnes M.S., Alexander H.D., McFaul P., Wallace J.M.W. Vitamin D de-ficiency and insufficiency in pregnant women: a longitudinal study. Br J Nutr. 2009; 102(6): 876–81. doi: 10.1017/S0007114509297236
- Karras S.N., Wagner C.L., Castracane V.D. Understanding vitamin D metabolism in pregnancy: from physiology to pathophysiology and clinical outcomes. Metabolism. 2018; 86: 112-23. doi: 10.1016/j.metabol.2017.10.001
- Ganguly A., Tamblyn J.A., Finn-Sell S., Chan S.Y., Westwood M., Gupta J., et al. Vitamin D, the placenta and early pregnancy: effects on tro-phoblast function. J Endocrinol. 2018; 236(2): R93-R103. doi: 10.1530/JOE-17-0491
- Norman A.W. From vitamin D to hormone D: fundamentals of the vitamin D en-docrine system essential for good health. Am J ClinNutr. 2008; 88(2): 491S-499S. DOI:10.1093/ajcn/88.2.491S
- Bakleicheva M., Kovaleva I., Bespalova O., Tolibova G., Tral T. The deficiency of Vitamin D with threatening miscarriage. The 3rd World Congress on Recurrent Pregnancy Loss (WCRPL 2018). Abstr. June 27-29, 2018, Madrid, Spain.
- Bikle D. Nonclassic actions of vitamin D. J Clin Endocrinol Metab. 2009; 94(1): 26-34. doi: 10.1210/jc.2008-1454
- Palacios C., Gonzalez L. Is vitamin D deficiency a major global public health problem? J Steroid BiochemMol Biol. 2014; 144Pt A:138-45. doi: 10.1016/j.jsbmb.2013.11.003
- Баклейчева М.О., Ковалева И.В., Беспалова О.Н., Коган И.Ю. Влияние витамина D на репродуктивное здоровье женщины. Журнал акушерства и женских болезней. 2018; 67(3): 4-19. [Bakleicheva M.O., Kovaleva I.V., Bespalova О.N., Kogan I.Yu. The effect of vitamin D on women’s reproductive health. Journal of Obstetrics and Women’s Diseases. 2018; 67(3): 4-19.(in Russ.)]. doi: 10.17816/JOWD6734-19
- Saraf R., Morton S.M.B., Camargo C.A., Grant C.C. Global summary of maternal and newborn vitamin D status – a systematic review. Matern Child Nutr. 2016; 12(4): 647-68. doi: 10.1111/mcn.12210
- Bakleicheva M., Kovaleva I., Bespalova O., Iyvashchenko T.,Tolibova G.Kh., Tral T.G. Тhe main reasons of threatening miscarriage between women with the vitamin D deficiency XXVI European Congress Perinatal Medicine ECPM Congress 2018. Abstract book [electronic version].2018; 350.
- Aghajafari F., Nagulesapillai T., Ronksley P.E., Tough S.C., O’Beirne M., Rabi D.M. Association between maternal serum 25-hydroxyvitamin D level and pregnancy and neonatal outcomes: systematic review and meta-analysis of observational studies. BMJ. 2013; 346: f1169-f1169. doi: 10.1136/bmj.f1169
- Theodoratou E., Tzoulaki I., Zgaga L., Ioannidis J.P.A. Vitamin D and multiple health outcomes: umbrella review of systematic reviews and meta-analyses of observational studies and randomisedtrials.BMJ. 2014; 348: g2035-g2035. doi: 10.1136/bmj.g2035
- De-Regil L.M., Palacios C, Lombardo L.K., Peña-Rosas J.P. Vitamin D supplementation for women during pregnancy. Cochrane Database Syst Rev. 2016; (1): CD008873.doi: 10.1002/14651858.CD008873.pub3
- Merewood A., Mehta S.D., Chen T.C., Bauchner H., Holick M.F. Association between vitamin D deficiency and primary cesarean section. J Clin Endocrinol Metab. 2009; 94(3): 940-5. doi: 10.1210/jc.2008-1217
- Bodnar L.M., Krohn M.A., Simhan H.N. Maternal vitamin D deficiency is associated with bacterial vaginosis in the first trimester of pregnancy. J Nutr. 2009;139(6):1157-61. doi: 10.3945/jn.108.103168
- Zehnder D., Evans K.N., Kilby M.D., Bulmer J.N., Innes B.A., Stewart P.M., Hewison M. The on-togeny of 25-hydroxyvitamin D(3) 1alpha-hydroxylase expression in human placenta and decidua. Am J Pathol. 2002; 161(1):105-14. DOI: 10.1016/s0002-9440(10)64162-4
- Gray T.K., Lester G.E., Lorenc R.S. Evidence for extrarenal 1-hydroxylation of 25-hydroxyvitamin D3 in pregnancy. Science. 1979; 204:1311–13. DOI: 10.1126/science.451538
- Adams J.S., Hewison M. Unexpected actions of vitamin D: new perspectives on the regulation of innate and adaptive immunity. Nat Clin Pract Endocrinol Metab. 2008; 4(2): 80-90. doi: 10.1038/ncpendmet0716
- Du H., Daftary G.S., Lalwani S.I., Taylor H.S. Direct regulation of HOXA10 by 1,25-(OH)2D3 in human myelomonocytic cells and human endometrial stromal cells. Mol Endocrinol. 2005; 19(9): 2222-33. https://doi.org/10.1210/me.2004-0336
- Bodnar L.M., Krohn M.A., Simhan H.N. Maternal vitamin D deficiency is associated with bacterial vaginosis in the first trimester of pregnancy. J Nutr. 2009; 139(6): 1157-61. doi: 10.3945/jn.108.103168
- Li N., Wu H.M., Hang F., Zhang Y.S., Li M.J. Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface. Braz J Med Biol Res. 2017; 50(11): e6527. doi: 10.1590/1414-431X20176527
Received 14.05.2019
Accepted 21.06.2019
About the Authors
Olesya N. Bespalova., MD, deputy director for research of the Research Institute of Obstetrics, Gynecology and Reproductology named after D.O.Ott,199034, Mendeleyevskaya Liniya 3, Saint-Petersburg, Russia. Тел.: +7 (911) 995-0096.. E-mail: shiggerra@mail.ru
Margarita O. Bakleicheva, resident doctor of the Research Institute of Obstetrics, Gynecology and Reproductology named after D.O.Ott,
199034, Mendeleyevskaya Liniya 3, Saint-Petersburg, Russia ORCID ID 0000-0002-0103-8583
Irina V. Kovalevа, gynecologoist, ООО «Skandinavia».
Gulrukhsor Kh. Tolibova, MD, senior research scientist of Department of Pathomorphology, the Research Institute of Obstetrics, Gynecology and Reproductology named
after D.O.Ott, 199034, Mendeleyevskaya Liniya 3, Saint-Petersburg, Russia
Tatiana G. Tral , PhD, the Head of Department of Pathomorphology, the Research Institute of Obstetrics, Gynecology and Reproductology named after D.O.Ott,
199034, Mendeleyevskaya Liniya 3, Saint-Petersburg, Russia
Igor Yu. Kogan, MD, corresponding member of RAS, professor, director of the Research Institute of Obstetrics, Gynecology and Reproductology named after D.O.Ott.
199034, Mendeleyevskaya Liniya 3, Saint-Petersburg, Russia
For citation: Bespalova O.N., Bakleicheva M.O., Kovaleva I.V., Tolibova G.Kh., Tral T.G., Kogan I.Yu. Expression of vitamin D and its receptors in the villous chorion in missed miscarriage.
Akusherstvo i Ginekologiya / Obstetrics and Gynecology. 2019; (11): 89-96.(in Russian).
http://dx.doi.org/10.18565/aig.2019.11.89-96
Similar Articles
