Placenta-specific micro-RNA expression in fetal growth restriction
Objective. To analyze the expression of placenta-specific microRNAs in pregnant women with fetal growth restriction (FGR) and with physiological pregnancy.Bushtyreva I.O., Kuznetsova N.B., Zabanova E.A., Butenko E.V., Pokudina I.O., Shkurat T.P.
Materials and methods. The investigation enrolled 42 women at 30-34 weeks’ gestation (27 with FGR and 15 with uncomplicated pregnancy), who were matched for age, anthropometric characteristics, features of an obstetric and gynecological history, and concomitant diseases. All the pregnant women with FGR were observed to have manifestations of different degrees of fetoplacental insufficiency. The patients underwent peripheral venous blood sampling. The expression of eight microRNAs (microRNA-10b-5p, microRNA-145-5p, microRNA-122-5p,
microRNA-141-3p, microRNA-125b-5p, microRNA-205-5p, microRNA-210-3p, and microRNA-517-5p) was determined by real-time polymerase chain reaction (PCR). The change in microRNA expression levels was estimated using the ∆ST method.
Results. It was ascertained that the expression of microRNA-125b-5p in the blood of pregnant women with
FGR was statistically significantly lower (12.3 (8.9; 13.8)) than that in women with physiological pregnancy
(8.1 (6.6; 9.1)) (p=0.011). The magnitude of expression change (fold change) for microRNA-125b-5p was 5.25.
Conclusion. The findings indicate a change in the microRNA-125b-5p expression levels in the pregnant women with FGR versus the women with uncomplicated pregnancy. The role of micro-RNA-125b-5p in the genesis
of FGR can be explained by the ability of this microRNA to affect the vascular endothelium, including the placental vasculature, disrupting the processes of endothelium-dependent relaxation, angiogenesis, endothelial cell proliferation, and other functions, including the regulation of platelet adhesion and aggregation.
Keywords
microRNA
fetal growth restriction
placenta
pregnancy complications
epigenetic regulation
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Received 16.09.2020
Accepted 25.12.2020
About the Authors
Natalia B. Kuznetsova, Dr. Med. Sci., Professor at the Department of Simulation education, RostSMU. Tel.: +7-928-770-97-62. E-mail: lauranb@inbox.ru.29 Nahichevansky ave., Rostov-on-Don, 344022, Russia.
Irina O. Bushtyreva, Dr. Med. Sci., Full Professor, Clinic of Professor Bushtyreva. Tel.: +7(863)288-00-00. E-mail: kio4@mail.ru.
58/7A Sobornyi ave., Rostov-on-Don, 344010, Russia.
Ekaterina A. Zabanova, Postgraduate at the Department of Simulation education, RostSMU. Tel.: +7(918)566-88-25. E-mail: rock-fe@mail.ru.
29 Nahichevansky ave., Rostov-on-Don, 344022, Russia.
Elena V. Butenko, Cand. Bio. Sci., Associate Professor at the Department of Genetics, Academy of Biology and Biotechnologies of SFedU.
Tel.: +7(909)420-22-23. E-mail: evbutenko@sfedu.ru. 194/1 Stachki ave., Rostov-on-Don, 344090, Russia.
Inna O. Pokudina, Cand. Bio. Sci., Senior researcher at the Laboratory of Biomedicine, Academy of Biology and Biotechnologies of SFedU.
Tel.: +7(918)504-56-50. E-mail: ipokudina@sfedu.ru. 194/1 Stachki ave., Rostov-on-Don, 344090, Russia.
Tatiana P. Shkurat, Dr. Bio. Sci., Full Professor, Director of Research Institute of Biology of SFedU. Tel.: +7(863)243-38-85. E-mail: tshkurat@sfedu.ru.
194/1 Stachki ave., Rostov-on-Don, 344090, Russia.
For citation: Bushtyreva I.O., Kuznetsova N.B., Zabanova E.A., Butenko E.V., Pokudina I.O., Shkurat T.P. Placenta-specific micro-RNA expression in fetal growth restriction.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2021; 2: 128-134 (in Russian)
https://dx.doi.org/10.18565/aig.2021.2.128-134
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