Time course of changes in the production of anti-leukocyte antibodies in pregnant women with idiopathic recurrent miscarriage during immunocytotherapy
Objective. To reveal regularities in the development of humoral immune responses during pregestational and gestational immunocytotherapy (ICT) in patients with recurrent miscarriage (RM), by estimating the level of antipaternal anti-leukocyte antibodies (anti-PAb).Krechetova L.V., Tetruashvili N.K., Vtorushina V.V., Nikolaeva M.A., Golubeva E.L., Saribegova V.A., Agadzhanova A.A., Vanko L.V.
Subjects and methods. Anti-PAbs were determined by cross-reactivity using flow cytometry at different stages of gestation in 85 control pregnant women and in 92 women with RM, among whom there were 69 women who received pregravid preparation (PP) involving immunization with their partner’s lymphocytes (ICT) as monotherapy or in combination with oral dydrogesterone, and 23 who had no PP.
Results. In the controls, anti-PAbs were detected in 5.9-7% of the pregnant women at less than 34 weeks’ gestation and in 25.9% at 38 weeks. The level of anti-PAbs at 5 to 6 weeks of gestation was significantly higher in the patients who received PP including ICT. In the patients who had no PP, the anti-PAB levels gradually increased after each immunization procedure in the first trimester to the level seen in those who received PP.
Conclusion. The emergence of anti-PAbs during pregnancy in patients with RM is the result of ICT, which indicates the importance of an anti-PAb test that reflects the immunomodulatory effect of an alloimmunization procedure.
Keywords
recurrent abortion
pregravid preparation
immunocytotherapy
antipaternal anti- leukocyte antibodies
flow cytometry
Supplementary Materials
- Table 1 – The course of pregnancy in the groups with pre-pregnancy preparation (PPP) and without it (WPPP)
- Table 2 – Pregnancy outcomes in groups with pre- pregnancy preparation (PPP) and without it (WPPP)
- Table 3 – Detection of antipaternal antibodies (APAB) during pregnancy
- Table 4 – Levels of APAB in patients with reccurent pregnancy loss (RPL) and women of control group in the dynamics of different gestation periods
- Fig.1 The dynamics of production APAB during pregnancy patients with RPL
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Received 07.04.2017
Accepted 28.04.2017
About the Authors
Krechetova Lyubov V., Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology, Research Center of Obstetrics, Gynecology and Perinatology,Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: k_l_v_@mail.ru
Tetruashvili Nana K., Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: tetrauly@mail.ru
Vtorushina Valentina V., Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology, Research Center of Obstetrics,
Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: vtorushina@inbox.ru
Nikolaeva Marina A., Doctor of Science, Leading researcher, Laboratory of Clinical Immunology, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: nikolaeva_ma@mail.ru
Golubeva Elena L., Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: e_golubeva@oparina4.ru
Saribegova Victoria A., Graduate student, Department of Pregnancy Loss Prevention and Therapy, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381477. E-mail: saribegova_v@rambler.ru
Agadzanova Anna, Doctor of Medicine, Doctor of Department of Pregnancy Loss Prevention and Therapy, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: aagadjanova@mail.ru
Vanko L.V., doctor of medicine, professor, leading research worker of clinical immunology laboratory, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: lvanko@mail.ru.
For citations: Krechetova L.V., Tetruashvili N.K., Vtorushina V.V., Nikolaeva M.A.,
Golubeva E.L., Saribegova V.A., Agadzhanova A.A., Vanko L.V. Time course of changes in the production of anti-leukocyte antibodies in pregnant women with idiopathic recurrent miscarriage during immunocytotherapy. Akusherstvo i Ginekologiya/
Obstetrics and Gynecology. 2017; (9): 48-55. (in Russian)
http://dx.doi.org/10.18565/aig.2017.9.48-55
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