Diagnostic significance of determining the level of extracellular fetal DNA in pregnant women with preeclampsia and fetal growth restriction

DOI

https://dx.doi.org/10.18565/aig.2019.8.144-149

Sadekova A.A., Khachatryan Z.V., Krasnyi A.M., Kan N.E., Khachaturyan A.A., Tyutyunnik V.L.
1) Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow; 2) N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow

Objective. To evaluate the diagnostic significance of determining extracellular fetal DNA in the plasma of pregnant women with preeclampsia and fetal growth restriction.
Subjects and methods. Plasma samples from 55 women were investigated during the study. The pregnant women were divided into groups: 1) 17 pregnant women with preeclampsia and fetal growth restriction; 2) 19 patients with a confirmed diagnosis of preeclampsia without fetal growth restriction; 3) 19 women with physiological pregnancy. Quantitative PCR analysis was used in all the patients to measure the plasma level of extracellular fetal DNA, by determining the concentration of the hypermethylated RASSF1A gene.
Results. The pregnant women with preeclampsia and growth restriction were found to have a significantly higher level of extracellular fetal DNA (284 GE/ml for impaired fetoplacental blood flow by Doppler ultrasound; 339 GE/ml for the absence of impaired fetoplacental blood flow) than those with preeclampsia (79 GE/ml) and apparently healthy pregnant women (25 GE/ml). The level of extracellular fetal DNA in pregnant women with preeclampsia and fetal growth restriction without impaired fetoplacental blood flow was shown to be an indicator of fetal growth restriction at a threshold of 201 GE/ml with a sensitivity of 80% and a specificity of 89%.
Conclusion. The findings indicate that quantitative determination of the plasma level of extracellular fetal DNA can be used to identify groups at risk for fetal growth restriction in pregnant women with preeclampsia. Quantification of the level of extracellular fetal DNA in combination with other methods for fetal assessment can assist in choosing the optimal timing and methods of delivery to improve perinatal outcomes.

extracellular fetal DNA

fetal growth restriction

preeclampsia

RASSF1

Full text (in Russian)

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Received 09.11.2018

Accepted 07.12.2018

Sadekova Alsu A., scientific researcher of the cytology laboratory of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. Tel.: +7(495)438-22-72. E-mail: a_sadekova@oparina4.ru 117997, Moscow, Ac. Oparina, 4 str.
Khachatryan Zarine V., postgraduate student of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. Tel.: +7(909)656-24-56. E-mail: z.v.khachatryan@gmail.com 117997, Moscow, Ac. Oparina, 4 str..
Krasnyi Aleksey M., PhD, the head of the cytology laboratory of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia., senior scientist, laboratory of evolutionary developmental biology, N.K. Koltzov Institute of Developmental Biology of Russian Academy of Sciences Tel.: +7(495)438-22-72. E-mail: alexred@list.ru 117997, Moscow, Ac. Oparina, 4 str. 119334, Moscow, Vavilova str. 26, Russia.
Kan Natalia E., PhD, MD, the head of the obstetric department of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. Tel.: +7-926-220-86-55. E-mail: kan-med@mail.ru. Number Researcher ID B-2370-2015.
ORCID ID 0000-0001-5087-5946 117997, Moscow, Ac. Oparina, 4 str.
Khachaturyan Anuta A., postgraduate student of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. Tel.: +7(926)845-39-53. E-mail: x.anyt37@mail.ru 117997, Moscow, Ac. Oparina, 4 str.
Tyutyunnik Victor L., PhD, MD, the head of the obstetric physiological department of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. Tel.: +7(903)969-50-41. E-mail: tioutiounnik@mail.ru. Number Researcher ID B-2364-2015.ORCID ID 0000-0002-5830-5099 117997, Moscow, Ac. Oparina, 4 str.

For citations: Sadekova A.A., Khachatryan Z.V., Krasnyi A.M.,Kan N.E., Khachaturyan A.A., Tyutyunnik V.L. Diagnostic significance of determining the level of extracellular fetal dna in pregnant women with preeclampsia and fetal growth restriction/ andintrauterine growth restriction.
Akusherstvo i Ginekologiya / Obstetrics and Gynecology. 2019; (8): 144-49 (in Russian)
http://dx.doi.org/10.18565/aig.2019.8.144-149

Diagnostic significance of determining the level of extracellular fetal DNA in pregnant women with preeclampsia and fetal growth restriction

Sadekova A.A., Khachatryan Z.V., Krasnyi A.M., Kan N.E., Khachaturyan A.A., Tyutyunnik V.L.

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