State of antimicrobial protection of vaginal secrete in pregnant women during treatment of bacterial vaginosis

DOI

https://dx.doi.org/10.18565/aig.2025.338

Tyutyunnik V.L., Mirzabekova D.D., Kan N.E., Khachatryan Z.V., Donnikov A.E.
1) Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, Moscow, Russia; 2) Perinatal Center of the European Medical Center, Moscow, Russia

Objective: A comprehensive analysis of the relationship between the antimicrobial activity of vaginal secretions and the clinical features of bacterial vaginosis in pregnant women during therapy, with consideration of vaginal pH dynamics and microbiological parameters.
Materials and methods: A total of 97 women participated in the study: 69 with bacterial vaginosis and 28 with normocenosis (normal vaginal flora). Patients with bacterial vaginosis were divided into a main group (n=37), who received combination therapy with an antibiotic and the immunomodulator "Superlymph", and a comparison group (n=32) on antibiotic monotherapy. After treatment, normocenosis was restored in all patients using the dosed cream "Acilact Duo". The antimicrobial activity of antimicrobial peptides was determined in the groups of pregnant women depending on the nature of the clinical symptoms of bacterial vaginosis against the background of its treatment.
Results: The results showed that the highest activity of antimicrobial peptides was observed in women with normocenosis. In bacterial vaginosis this activity decreased in proportion to the severity of the disease. In the main group, after combination therapy, this indicator increased almost twofold from the baseline. This regimen provided a significant improvement in clinical and laboratory parameters, a reduction in recurrences, prolonged remission, and restoration of the microbiota.
Conclusion: Including an assessment of immune status in the diagnosis of bacterial vaginosis in pregnant women, alongside a comprehensive evaluation of the vaginal microbiome, allows for personalized therapy. The results substantiate the advisability of implementing combination therapy into clinical practice.

Authors’ contributions: Tyutyunnik V.L., Mirzabekova D.D., Kan N.E., Khachatryan Z.V., Donnikov A.E. – developing of study design, obtaining data for analysis, reviewing publications on the topic of the article, statistical analysis of the obtained data, article writing.
Conflicts of interest: Authors declare lack of the possible conflicts of interest.
Funding: The study was conducted without sponsorship.
Ethical Approval: The study was approved by the ethics committee of the Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
Patient Consent for Publication: All patients provided informed consent for the publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Tyutyunnik V.L., Mirzabekova D.D., Kan N.E., Khachatryan Z.V., Donnikov A.E. State of
antimicrobial protection of vaginal secrete in pregnant women during treatment of bacterial vaginosis.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2025; (12): 53-62 (in Russian)
https://dx.doi.org/10.18565/aig.2025.338

bacterial vaginosis

antimicrobial peptides

antibacterial therapy

pregnancy

immunomodulatory therapy

Superlymph

Acilact Duo

Full text (in Russian)

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Received 20.11.2025

Accepted 15.12.2025

Victor L. Tyutyunnik, Professor, Dr. Med. Sci., Leading Researcher at the Research and Development Service, Academician V.I. Kulakov National Medical Research Center
for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparina str., 4, tioutiounnik@mail.ru, Researcher ID: B-2364-2015,
SPIN: 1963-1359, Authors ID: 213217, Scopus Author ID: 56190621500, https://orcid.org/0000-0002-5830-5099
Dzhamilia D. Mirzabekova, PhD, obstetrician-gynecologist at the 1st Obstetric Physiological Department, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparina str., 4, Jamilya1705@yandex.ru,
https://orcid.org/0000-0002-2391-3334
Natalia E. Kan, Professor, Dr. Med. Sci., Honored Scientist of the Russian Federation, Deputy Director General for Research – Director of the Institute of Obstetrics, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow,
Ac. Oparina str., 4, kan-med@mail.ru, Researcher ID: B-2370-2015, SPIN: 5378-8437, Authors ID: 624900, Scopus Author ID: 57008835600,
https://orcid.org/0000-0001-5087-5946
Zarine V. Khachatryan, PhD, obstetrician-gynecologist of the Maternity Department, Perinatal Center of the European Medical Center, 125040, Russia, Moscow, Pravdy str., 15, build. 1, z.v.khachatryan@gmail.com, https://orcid.org/0009-0000-2652-6156
Andrey E. Donnikov, PhD, Head of the Laboratory of Molecular Genetic Methods, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparina str., 4, donnikov@dna-technology.ru, https://orcid.org/0000-0003-3504-2406

State of antimicrobial protection of vaginal secrete in pregnant women during treatment of bacterial vaginosis

Tyutyunnik V.L., Mirzabekova D.D., Kan N.E., Khachatryan Z.V., Donnikov A.E.

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