Correlation of perinatal outcomes in preeclampsia with the dynamics of CD16+ monocyte content in peripheral blood
Tyutyunnik V.L., Mirzabekova D.D., Mikhailova O.I., Kan N.E., Krasnyi A.M.
Objective: To study the changes of CD16+ monocytes, the expression of CD86, CD152 in monocytes and CD28 in lymphocytes in the peripheral blood of pregnant women with preeclampsia in the course of therapy; to identify factors of prognostic value for determining the group of patients with complications in the neonatal period.
Materials and methods: The study included 26 patients with preeclampsia of various degrees of severity and time of manifestation. The expression of CD86 and CD152 in monocytes, CD28 in lymphocytes, and CD16+ in monocytes was determined using flow cytometry after diagnosis verification and before delivery. Two study groups were formed retrospectively: group 1 (main, n=10) included patients with a complicated course of the early neonatal period and group 2 (comparison group, n=16) included patients with an uncomplicated course of the early neonatal period. The change in the markers in response to therapy in both study groups was examined.
Results: There was a higher level of increase in the studied markers in the group of patients with a complicated course of the early neonatal period. There was a statistically significant increase in the CD16+ monocyte content in the group of patients with a complicated course (p<0.001). The median change in the content of CD16+ monocytes over five days was 13%. It was 0.95% in the group of patients with an uncomplicated course of the early neonatal period. The ROC analysis showed that the time change in the content of CD16+ in monocytes has a prognostic value for determining perinatal outcomes in preeclampsia, AUC=0.96.
Conclusion: The findings suggest the prospective prognostic value of determining the level of CD16+ monocytes in the peripheral blood of pregnant women in dynamics in order to predict perinatal outcomes. This can help optimize the planned delivery time.
Authors’ contributions: Tyutyunnik V.L., Mikhailova O.I., Kan N.E., Mirzabekova D.D., Krasnyi A.M. – developing the concept and design of the study, obtaining data for analysis, review of publications on the topic of the article, processing and analysis of the material, writing the text, editing the article.
Conflicts of interest: The authors declare no possible conflicts of interest.
Funding: The study was conducted without sponsorship.
Ethical Approval: The study was approved by the Ethical Review Board of the Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
Patient Consent for Publication: The patients provided an informed consent for the publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Tyutyunnik V.L., Mirzabekova D.D., Mikhailova O.I., Kan N.E., Krasnyi A.M. Correlation of perinatal outcomes in preeclampsia with the dynamics of CD16+ monocyte content in peripheral blood.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2024; (9): 73-80 (in Russian)
https://dx.doi.org/10.18565/aig.2024.168
Keywords
pregnancy
preeclampsia
peripheral blood mononuclear cells
CD152
CD86
CD28
CD16
monocytes
lymphocytes
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Received 17.07.2024
Accepted 24.09.2024
About the Authors
Victor L. Tyutyunnik, Professor, Dr. Med. Sci., Leading Researcher of Center of Scientific and Clinical Researches, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, tioutiounnik@mail.ru, Researcher ID: B-2364-2015, SPIN-code: 1963-1359, Authors ID: 213217, Scopus Author ID: 56190621500, https://orcid.org/0000-0002-5830-5099Dzhamilia D. Mirzabekova, graduate student, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, Jamilya1705@yandex.ru, https://orcid.org/0000-0002-2391-3334
Natalia E. Kan, Professor, Dr. Med. Sci., Deputy Director of Science, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, kan-med@mail.ru, Researcher ID: B-2370-2015, SPIN-code: 5378-8437,
Authors ID: 624900, Scopus Author ID: 57008835600, https://orcid.org/0000-0001-5087-5946
Olga I. Mikhailova, PhD, Researcher at 2nd Maternity Department, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, omikhaylova@gmail.com, https://orcid.org/0000-0001-7569-8704
Aleksey M. Krasnyi, PhD, Head of the Cytology Laboratory, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia, 117997, Russia, Moscow, Ac. Oparin str., 4, alexred@list.ru, https://orcid.org/0000-0001-7883-2702
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