Comparative analysis of the lipid composition of peritoneal fluid and blood plasma in patients with external genital endometriosis and uterine myoma
Objective. To enhance the efficiency of diagnosing external genital endometriosis by direct mass spectrometry.Borisova A.V., Chagovets V.V., Kozachenko A.V., Starodubtseva N.L., Kononikhin A.S., Salimova D.F., Kogan E.A., Adamyan L.V., Frankevich V.E., Sukhikh G.T.
Subjects and methods. A case-control study covered 100 patients with external genital endometriosis (a study group) and 50 patients with uterine myoma (a control group) who had been operated on at the Gynecology Department, V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation. The diagnosis of external genital endometriosis was based on diagnostic and therapeutic laparoscopy and finally confirmed histologically. To determine the lipid composition of blood and peritoneal fluid, their samples were obtained from all the study participants. The lipidome was qualitatively and quantitatively assessed using electrospray mass spectrometry. An analysis revealed more than 140 molecular characteristics, most of which were five lipid classes: phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, diglycerides, and triglycerides. The obtained experimental data were analyzed using the multivariate partial lest squares-discriminant analysis (PLS-DA). The created statistical models well group data based on a case-control sign and could identify lipids, which make the greatest contribution to the clustering of the data.
Results. Unlike the comparison group, the patients with endometriosis showed a substantial decrease in the blood and peritoneal fluid level of four lipids: phosphatidylcholine PC 36:4, lysophosphatidylcholine LPC 16:0, sphingomyelin SM 34:1, and phosphoethanolamine PE O-34:1. The level of phosphoethanolamine PE O-20:0 was increased not only in the endometrial tissues, but also in the biological fluids of patients with endometriosis, suggesting the possible diagnostic value of this lipid. The sensitivity and specificity of the method was 93% and 95%, respectively for blood plasma; those were 90 and 95% for peritoneal fluid.
Conclusion. This investigation confirms the involvement of phospholipids and sphingolipids in the pathophysiology of endometriosis and opens up new possibilities for the noninvasive diagnosis of external genital endometriosis.
Keywords
endometriosis
biomarkers
mass spectrometry
phosphatidylcholine
phosphatidylethanolamine
lysophosphatidylcholine
sphingomyelin
Supplementary Materials
- Fig. 1. Chart of accounts, based on the results of PLS-DA analysis of blood plasma (a) and peritoneal fluid (b) in patients with endometriosis and women in the control group
- Fig. 2. Comparison of lipid levels that make the greatest contribution to the separation of data on the sign of endometriosis-control of patients with endometriosis and women of the control group, (a) in blood plasma; (B) in the peritoneal fluid
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Received 10.02.2017
Accepted 17.02.2017
About the Authors
Borisova Anna Valerevna, PhD student, Research Center of Obstetrics, Gynecology and Perinatology, Ministry Of Health of Russia.117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79267010504. E-mail: aneta_b@mail.ru
Kozachenko Andrey Vladimirovich, MD, PhD, Senior Researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry Of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79166144132. E-mail: andreykozachenko@list.ru
Starodubtseva Nataliia Leonidovna, PhD, Head of Laboratory of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry Of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79164639867. E-mail: n_starodubtseva@oparina4.ru
Kogan Eugenia Aleksandrovna, MD, PhD, professor, Head of the Department of Pathological Anatomy of the medical faculty of I.M. Sechenov First Moscow State Medical University. 119991, Russia, Moscow, Malaya Pirogovskaya str. 8/21. Tel.: +79265331271. E-mail: koganevg@gmail.com
Chagovets Vitaliy Viktorovich, PhD, Senior Researcher of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry Of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79265626590. E-mail: vvchagovets@gmail.com
Salimova Dinara Failevna, Student of I.M. Sechenov First Moscow State Medical University.
119991, Russia, Moscow, Malaya Pirogovskaya str. 8/21. Tel.: +79162060766. E-mail: Salimova.1993@mail.ru
Kononikhin Alexey Sergeevich, PhD, Researcher of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry Of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79167854781. E-mail: a_kononihin@oparina4.ru
Frankevich Vladimir Evgenievich, PhD, Head of Department of Systems Biology in Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry Of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380788, ext. 2198. E-mail: v_frankevich@oparina4.ru
Adamyan Leila Vladimirovna, Academician of the Russian Academy of Sciences, Professor, Honoured Scientist of the Russian Federation, Deputy Director for Scientific Affairs, Research Center of Obstetrics, Gynecology and Perinatology, Ministry Of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954389400. E-mail: adamyanleila@gmail.com
For citations: Borisova A.V., Chagovets V.V., Kozachenko A.V.,
Starodubtseva N.L., Kononikhin A.S., Salimova D.F., Kogan E.A., Adamyan L.V.,
Frankevich V.E., Sukhikh G.T. Comparative analysis of the lipid composition of peritoneal fluid and blood plasma in patients with external genital endometriosis and uterine myoma.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (6): 74-82. (in Russian)
http://dx.doi.org/10.18565/aig.2017.6.74-82
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