Results of preimplatation genetic screening of embryos in married couples with sperm DNA fragmentation
Objective. To determine the frequency of aneuploidies (monosomies, trisomies, disomies, nullisomies) from all chromosomes, as well as DNA deletions and amplifications in the embryos from couples with higher and normal sperm nuclear DNA fragmentation levels.Kiseleva Yu.Yu., Azova M.M., Kodyleva T.A., Ushakova I.V., Kirillova A.O., Ekimov A.N., Rakitko A.S., Volodyaeva T.O., Mishieva N.G., Abubakirov A.N.
Subjects and methods. An array-based preimplatation genetic screening program was applied to 170 couples; each couple underwent estimation of sperm concentration, motility and morphology according to the 2010 WHO standards, as well as sperm nuclear DNA fragmentation levels by the TUNEL method.
Results. A relationship was found between the level of paternal sperm DNA fragmentation and the frequency of obtaining embryos with a normal karyotype. With the increased level of DNA fragmentation, this figure is 47.96% vs 66.17% with its normal level (p < 0.02). There was also a substantially increased frequency of embryo DNA deletions and amplifications with a higher level of paternal sperm DNA fragmentation (35.89% vs 5.39% with its normal level; p < 0.001). Fragmentation does not have a statistically significant impact on abnormal chromosome number.
Conclusion. This study has demonstrated a correlation between the level of paternal sperm DNA fragmentation and the frequency of structural karyotype abnormalities in embryos.
Keywords
DNA fragmentation
preimplatation genetic screening
aneuploidies
aCGH
TUNEL
Supplementary Materials
References
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Received 03.10.2016
Accepted 11.11.2016
About the Authors
Kiseleva Yulia Yu., embryologist, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; graduate student of the Department of Biology and General Genetics Medical Faculty, Peoples’ Friendship University. 117997, Russia, Moscow, Ac. Oparina str. 4. Тel.: +79164171311. Е-mail: 79164171311@yandex.ruAzova Madina M., DSc, Associate Professor, Head of the Department of Biology and General Genetics Medical Faculty, Peoples’ Friendship University.
117198, Russia, Moscow, Miklukho-Maklaya str. 6. Tel.: +74954345300.E-mail: azovam@mail.ru
Kodyleva T.A., embryologist 1st gynecology department, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382622. E-mail: t_kodyleva@oparina4.ru
Ushakova I.V., Ph.D., embryologist, department of reproductive, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79859289287. E-mail: ir_ushakova@oparina4.ru
Kirillova Anastasiya O., embryologist, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Тel.: +74954382622. Е-mail: a_kozyreva@oparina.ru
Ekimov Alexey N., researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Тel.: +74954382622. Е-mail: a_ekimov@oparina4.ru
Rakitko Aleksandr S., PhD student, Department of Theory of Probability, Faculty of Mathematics and Mechanics, M.V. Lomonosov Moscow State University.
119991, Russia, Moscow, Leninskie Gory, 1. Tel.: +79104820017. E-mail: rakitko@gmail.com
Volodyaeva Tatiana O., embryologist, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Тel.: +79993255305. Е-mail: tvolo90@mail.ru
Mishieva Nona G., MD, Senior researcher of 1st gynecology department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Тel.: +79104244197. E-mail: nondoc555@mail.ru
Abubakirov Aydar N., PhD, Head of 1-st gynecology department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Тel.: +74954382622. Е-mail: nondoc555@yahoo.com
For citations: Kiseleva Yu.Yu., Azova M.M., Kodyleva T.A., Ushakova I.V., Kirillova A.O.,
Ekimov A.N., Rakitko A.S., Volodyaeva T.O., Mishieva N.G., Abubakirov A.N. Results of preimplatation genetic screening of embryos in married couples with sperm DNA fragmentation.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (8): 104-8. (in Russian)
http://dx.doi.org/10.18565/aig.2017.8.104-8
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