Direct mass spectrometry approach for the express identification of tumor tissue from breast cancer patients
Objective. The development of analytical approach based on direct mass spectrometry analysis for the rapid identification and differentiation between tumor and healthy breast tissue.Tokareva A.O., Chagovets V.V., Zhihao Wang, Rodionov V.V., Kometova V.V., Rodionova M.V., Kononikhin A.S., Starodubtseva N.L., Chingin K., Frankevich V.E., Huanwen Chen, Sukhikh G.T.
Subjects and methods. The case-control study included 25 patients with invasive breast cancer. Each sample of tumor and healthy tissue from each patient was divided into two parts. One part was used for pathomorphological examination. Another part was investigated by direct mass spectrometry analysis to obtain molecular profile. The mass spectrometric data were processed by the OPLS-DA multivariate analysis.
Results. The normal and tumor breast tissues were differentiated by OPLS-DA analysis of the mass spectrometric data. Lipids of 4 different classes contributed in classification: diacylglycerol DG 34:1, monoacylglycerols MG 18:1, MG O-14:1, phosphatidylcholines LPC 16:0, PC 32:0, PC 32:1, PC 34:0, PC 34:1, PC 34:2, PC 36:3, PC 38:3, PC 38:4, sphingomyelin SM 32:1.
Conclusions. Direct mass spectrometry tissue analysis allows differentiation between tumor and healthy breast tissues based on characteristic patterns of lipid ion signals in the mass spectrum. Besides their diagnostic value, these characteristic patterns can also be used for the mechanistic study of tumor pathogenesis.
Keywords
breast cancer
direct mass spectrometry
lipidomics
phosphatidylcholine
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Received 07.02.2017
Accepted 17.02.2017
About the Authors
Tokareva Alisa Olegovna, student, Moscow Institute of physics and technology.141700, Russia, Moscow region, Dolgoprudny, Institutsky lane 9. Tel.: +79651286886. E-mail: alisa.tokareva@phystech.edu
Chagovets Vitaliy Viktorovich, PhD, Senior Researcher of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79265626590. E-mail: vvchagovets@gmail.com
Zhihao Wang, student, East China Institute of Technology, Nanchang, China. Tel.: +79265626590. E-mail: frankevich@rambler.ru
Rodionov Valeriy Vitalievich, MD, Head of the Department of Breast Pathology, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79265626590. E-mail: V_Rodionov@oparina4.ru
Kometova Vlada Vladimirovna, MD, Senior Researcher of Department of Pathological Anatomy, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79265626590. E-mail: v_kometova@oparina4.ru
Rodionova Maria Valerievna, MD, oncologist of the Department of Breast Pathology, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: 9265626590. E-mail: m_Rodionova@oparina4.ru
Kononikhin Alexey Sergeevich, PhD, Researcher of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79167854781. E-mail: a_kononihin@oparina4.ru
Starodubtseva Nataliia Leonidovna, PhD, Head of Laboratory of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79164639867. E-mail: n_starodubtseva@oparina4.ru
Chingin Konstantin, professor, East China Institute of Technology, Nanchang, China.
Tel.: +79265626590. E-mail: chingin.k@hotmail.com
Frankevich Vladimir Evgenievich, PhD, Head of Department of Systems Biology in Reproduction, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380788, ext. 2198. E-mail: v_frankevich@oparina4.ru
Huanwen Chen, professor, East China Institute of Technology, Nanchang, China. Tel.: +79265626590. E-mail: chingin.k@hotmail.com
Sukhikh Gennadiy Tikhonovich, Academician of RAS, MD, PhD, Professor, Director, Research Center of Obstetrics, Gynecology and Perinatology,
inistry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381800. E-mail: g_sukhikh@oparina4.ru
For citations: Tokareva A.O., Chagovets V.V., Zhihao Wang, Rodionov V.V.,
Kometova V.V., Rodionova M.V., Kononikhin A.S., Starodubtseva N.L., Chingin K.,
Frankevich V.E., Huanwen Chen, Sukhikh G.T. Direct mass spectrometry approach
for the express identification of tumor tissue from breast cancer patients.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (4): 119-25. (in Russian)
http://dx.doi.org/10.18565/aig.2017.4.119-25
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