IVF programs using embryos obtained from vitrified and native donor oocytes
Objective. To analyze the laboratory and clinical outcomes of IVF-DO programs using embryos from vitrified donor oocytes and to compare them with the results of those with native and thawed embryos derived from native donor oocytes.Krasnopolskaya K.V., Nazarenko T.A., Sesina N.I., Aleksandrova V.R.
Material and methods. The results of IVF-DO programs using vitrified oocytes (29 oocyte thaw cycles), native embryos (115 embryo transfer (ET) cycles), and thawed embryos derived from native oocytes (56 ET cycles) were compared. The survival and fertilization rates of thawed vitrified oocytes were clarified in assessing the results. When the clinical outcomes of the IVF-DO programs compared were assessed, the rates of ET discontinuation, pregnancy per ET, implantation, multiple pregnancy, and early reproductive losses were analyzed.
Results. The survival and fertilization rates of vitrified oocytes were ascertained to be 88.7 and 67%, respectively. The clinical outcomes of IVF-DO using the embryos derived from vitrified oocytes were substantially inferior to those with native or thawed embryos obtained from native oocytes in terms of all the most important indicators. Treatment results in view of pregnancy rates in the IVF-DO programs with vitrified oocytes can be improved only by increasing the number of transferred embryos to three; however, there is a dramatic increase in multiple pregnancy risk that is much greater than in the alternative IVF-DO programs with native or thawed embryos derived from freshly isolated oocytes.
Conclusion. In all situations when the use of donor oocytes is required to overcome infertility, it is advisable to give priority to IVF-DO programs using just native or thawed embryos obtained from native oocytes.
Keywords
in vitro fertilization
donor oocytes
vitrification
Supplementary Materials
- Table 1. The result of cycles IVM with donor oocytes, which obtained from verification donor oocytes.
- Table 2. The result of cycles IVM with donor oocytes, which obtained from verification donor oocytes.
- Table 3. The results of the program IVM-with donor oocyte using of fresh and defrozen embryos from fresh donor oocytes.
References
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Received 04.08.2016
Accepted 09.02.2016
About the Authors
Krasnopolskaya K.V., Ph.D., Professor, Head of the Department of Defense Reproduction, Moscow Regional Research Institute of Obstetrics and Gynecology.101000, Russia, Moscow, Pokrovka str. 22a. E-mail: deti222@mail.ru
Nazarenko T.A., MD, professor, leading researcher Branch Reproduction, Moscow Regional Research Institute of Obstetrics and Gynecology.
101000, Russia, Moscow, Pokrovka str. 22a. E-mail: deti222@mail.ru
Sesina N.I., head of the „Family” embryology laboratory of the International Clinic.
129110, Russia, Moscow, Bolshaya Pereyaslavskaya str. 7, bldg. 1. E-mail: biooptima@gmail.com
Alexandrova V.R., post-graduate, department of reproduction, Moscow Regional Research Institute of Obstetrics and Gynecology.
101000, Russia, Moscow, Pokrovka str. 22a. E-mail: deti222@mail.ru
For citations: Krasnopolskaya K.V., Nazarenko T.A., Sesina N.I., Aleksandrova V.R. IVF programs using embryos obtained from vitrified and native donor oocytes. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (3): 75-80. (in Russian)
http://dx.doi.org/10.18565/aig.2017.3.75-80
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