Features of the myometrial status during cesarean section with regard to amniorrhea and birth activity: A clinical and morphological study

Prikhodko A.M., Baev O.R., Karapetyan A.O., Demura T.A., Kogan E.A.

1 Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow 117997, Ac. Oparina str. 4, Russia; 2 I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia, Moscow 119991, Trubetskaya str. 8-2, Russia

Different factors caused by both equipment and the course of surgery (conditions under which the operation is performed, the location of incision, the characteristics of suture material, the type of surgical suture, and the amount of blood loss), by the course of the postoperative period, and the peculiarities of repair of damaged tissues influence wound healing of the uterus during cesarean section.
Objective. To establish the value of premature amniorrhea and uterine inertia as predictors of impaired myometrial repair after cesarean section, by using clinical and morphological analyses.
Subjects and methods. The investigation enrolled 129 patients who had given birth via cesarean section. Of them, 44 patients had delivery before birth activity, 85 during the first stage of labor. 49 and 80 women delivered before and after amniorrhea, respectively. During cesarean section, uterine tissue was taken from the upper edge of the wound after uterine incision. The myometrial biopsy specimens obtained during cesarean section were morphologically and immunohistochemically examined. The patients were divided into 4 groups according to the level of birth activity and the preservation of amniotic fluid at the time of cesarean section. Group 1 included patients with regular labor activity and amniorrhea at the time of caesarean section; Group 2 consisted of those with labor activity in the presence of whole amniotic fluid; Group 3 comprised those without birth activity in the presence of whole amniotic fluid; Group 4 included patients with premature amniorrhea without uterine contractions. 36 cases (9 in each group) were selected by random sampling for morphological and immunohistochemical examinations. The biopsy specimens were fixed in 10% neutral formalin and embedded in paraffin. The serial paraffin-embedded sections underwent histological examination and immunohistochemical tests for the following markers: TGF-β, VEGF, MMP2, TIMP1, types I and III collagen, TNF, and PDGF.
Results. The morphological and immunohistochemical analyses revealed the most pronounced signs of myometrial damage during cesarean section in Group 4 patients having premature amniorrhea without uterine contractions. There were decreased VEGF, PDGF, MMP2, and TIMP levels and simultaneously increased TNF-α expression in leiomyocytes, vascular endothelium, and myometrial stromal cells. The findings may indicate the relatively lower reparative potential of the myometrium and the increased readiness for an inflammatory response in the group of women undergoing cesarean section in the presence of premature amniorrhea without uterine contractions.
Conclusion. Clinical, morphological, and immunohistochemical analyses have revealed differences in the myometrial status in relation to typical clinical factors, such as amniorrhea and birth activity. Wound healing occurs under the influence of growth factors and the ratio of expression levels for growth factors can vary in different pathological conditions. The reduced expression of VEGF, MMP2, TIMP, and PDGF and the increased expression of TNF in the group having amniorrhea without uterine contractions (P-B+) suggest that there are pronounced inflammatory processes and impaired myometrial repair with the longer latency period in the absence of labor activity, which may refer these women to a group at risk for incompetent scar formation.

Keywords

Supplementary Materials

1. Fig. 1 Myometrium of the 4th patients group; Leiomyocytes hypertrophy, vessels plethora, edema and degeneration of the myometrium by 1 point, x250, staining with hematoxylin and eosin.
2. Fig. 2 Myometrium of the 2th patients group; Leiomyocytes hypertrophy, vessels plethora, edema and degeneration of the myometrium by 4 points, x250, staining with hematoxylin and eosin.
3. Fig. 3. Analysis of TIMP1 and MMP immunohistochemical expression in the myometrium leiomyocytes and vascular endothelium.
4. Fig. 4 MMP2 in the myometrium leiomyocytes and vascular endothelium: low (a) and moderate expression (b), immunoperoxidase reaction, x200.
5. Fig. 5 TIMP1 in the myometrium leiomyocytes and vascular endothelium: low expression in 4th patients group (a) and moderate expression in 1th patients group (b), immunoperoxidase reaction, x200, x100.
6. Fig. 6 Analysis of collagen I and III immunohistochemical expression in the myometrium.
7. Fig. 7 Analysis of TNF immunohistochemical expression in the myometrium.
8. Fig. 8 TNF in the myometrium: low expression in 1th patients group (a) and moderate expression in 4th patients group (b), immunoperoxidase reaction, x200.
9. Fig. 9 Analysis of PDGF immunohistochemical expression in the myometrium.

References

About the Authors

Prikhodko Andrey, obstetrician-gynecologist of Maternity Department, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954383047. E-mail: a_prikhodko@oparina4.ru
Baev Oleg, MD, Phd, professor, the head of Maternity Department, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954383047. E-mail: metod_obsgyn@hotmail.com
Karapetyan Anna Ovikovna, the postgraduate student, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79057068481. E-mail: anne-89@mail.ru
Demura Tatyana, MD, PhD, professor, A.I. Strukov Department of Anatomic Pathology, I.M. Sechenov First Moscow State Medical University.
119991, Russia, Moscow, Trubetskaya str. 8-2. Tel.: +74992480181. E-mail demura-t@yandex.ru
Kogan Evgeniya, MD, Phd, professor, Head of A.I. Strukov Department of Anatomic Pathology, I.M. Sechenov First Moscow State Medical University.
119991, Russia, Moscow, Trubetskaya str. 8-2. Tel.: +74992480181. E-mail koganevg@gmail.com

For citations: Prikhodko A.M., Baev O.R., Karapetyan A.O., Demura T.A., Kogan E.A. Features of the myometrial status during cesarean section with regard to amniorrhea and birth activity: A clinical and morphological study. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (4): 50-7. (in Russian)
https://dx.doi.org/10.18565/aig.2018.4.50-57

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