Peculiarities of laboratory diagnostics of anti-Müllerian hormone levels
Yarmolinskaya M.I., Cherkashina S.A.
The assessment of a woman’s reproductive potential using the serum anti-Müllerian hormone (AMH) level remains an important clinical issue. Various laboratory techniques are used to determine the concentration of AMH. However, the results obtained from these techniques are not standardized or consistent. This can lead to different numerical values when the same sample is studied using various analytical systems.
The threshold values indicated in clinical guidelines are often provided without any information about the specific research methodology and a comment on possible differences between laboratory methods. It is important for clinicians to pay attention to the differences in results obtained using various techniques.
The review examines issues related to ovarian reserve and folliculogenesis, the chemical structure of AMH as well as the characteristics of fluctuations in its levels. It also compares modern methods for detecting AMH in blood and considers the opportunity of their practical application.
Conclusion: The absence of an international standard for measuring AMH and the presence of different immunoassay methods which are used to determine its level emphasize the need to take into consideration the analytical system factor. This factor is especially important in the development of clinical strategies and search for optimal approaches to measurement standardization.
Authors’ contributions: Yarmolinskaya M.I., Cherkashina S.A. – developing the concept and design of the study, data collection, analysis of data and drawing conclusions, preparation of the manuscript.
Conflicts of interest: The authors declare no possible conflicts of interest.
Funding: This work was performed within the Basic Scientific Research FGWN-2025-0004 1024032800068-4-3.2.2.
For citation: Yarmolinskaya M.I., Cherkashina S.A. Peculiarities of laboratory diagnostics of anti-Müllerian hormone levels. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2025; (8): 223-228 (in Russian)
https://dx.doi.org/10.18565/aig.2025.189
Keywords
References
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Received 10.07.2025
Accepted 28.07.2025
About the Authors
Maria I. Yarmolinskaya, Dr. Med. Sci., Professor of the Russian Academy of Sciences, Honored Scientist of the Russian Federation, Head of the Department of Gynecology and Endocrinology, Head of the Center of Diagnostics and Treatment of Endometriosis, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,199034, Russia, St. Petersburg, Mendeleevskaya line, 3, m.yarmolinskaya@gmail.com, SPIN-код: 3686-3605, Researcher ID: P-2183-2014, Scopus Author ID: 7801562649, https://orcid.org/0000-0002-6551-4147
Sofya A. Cherkashina, doctor at the Consultative and Diagnostic Department No. 2, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleevskaya line, 3, sonyach@list.ru, https://orcid.org/0009-0005-7208-3575
Corresponding author: Maria I. Yarmolinskaya, m.yarmolinskaya@gmail.com