Placental PIBF1 gene mRNA expression during preterm labor
Objective. To investigate the role of a progesterone-induced blocking factor in the genesis of preterm labor.Dobrokhotova Yu.E., Trofimov D.Yu., Shchegolev A.I., Burmenskaya O.V., Veselovskaya Yu.S., Mitrofanova Yu.V., Olenev A.S., Pastarnak A.Yu., Gogichaev T.K.
Subjects and methods. A comprehensive examination was made in 63 puerperas (34 with preterm labor and 29 with term labor).
Results. There was evidence for the effects of micronized progesterone on placental PIBF1 gene mRNA expression. A direct correlation (correlation coefficient r = 0.66 (95% CI 0.22 to 1.12)) was found between plasma progesterone levels and placental PIBF1 gene mRNA expression. Patients with low PIBF1 gene mRNA expression were ascertained to have histological signs of placental insufficiency.
Conclusion. In the presence of progesterone deficiency, the reduced placental PIBF1 gene mRNA expression could lead to the breakdown of maternal adaptive reactions aimed at maintaining pregnancy and will be one of the factors that initiate preterm labor.
Keywords
preterm labor
micronized progesterone
progesterone
progesterone-induced blocking factor
placental insufficiency
Supplementary Materials
- Fig. 1. Correlation between blood progesterone level (nmol / L) and mRNA expression of PIBF1 gene, standard units
- Fig. 2. Expression of the mRNA of the PIBF1 gene (unit units) in the placenta in patients in the study groups
- Fig. 3. Expression of the mRNA of the PIBF1 gene in the placenta in patients with an abortion in an anamnesis
- Рис. 4. Уровень прогестерона в периферической плазме, нмоль/л
- Fig. 5. Preparation of placenta patient with preterm labor at 34-35 weeks. Staining with hematoxylin and eosin. Signs of placental insufficiency: the presence of fibrinoid degeneration zones in the field of view with the precipitation of calcium salts, the prevalence of intermediate villi
- Fig. 6. Preparation of placenta patient with premature birth at 34-35 weeks. Staining with hematoxylin and eosin. The manifestation of compensatory reactions in placental insufficiency: syncytial kidney, syncytocapillary membrane
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Received 16.12.2016
Accepted 23.12.2016
About the Authors
Dobrokhotova Yulia E., MD, Head of the Department of Obstetrics and Gynecology of the Medical Faculty of N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanova str. 1. Tel.: +74952374033. E-mail: pr.dobrohotova@mail.ruTrofimov Dmitry Y., Doctor of Biological Sciences, Head of the Laboratory of Molecular Genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: d.trofimov@dna-tech.ru
Schegolev Alexander I., MD, Professor, Head of the 2nd Pathoanatomical Department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382892. E-mail: ashegolev@oparina4.ru
Burmenskaya Olga V., Doctor of Biological Sciences, Researcher of the Laboratory of Molecular Genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: o_bourmenskaya@oparina4.ru
Veselovskaya Yulia S., post-graduate student of the Department of Obstetrics and Gynecology of the Faculty of Medicine, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanova str. 1. E-mail: isveselovskaia@gmail.com
Mitrofanova Yulia V., post-graduate student of the Department of Obstetrics and Gynecology of the Medical Faculty, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanova str. 1
Olenev Anton S., Candidate of Medical Science, Deputy chief doctor of the City Clinical Hospital No. 24, the head of the Perinatal Center branch.
127287, Russia, Moscow, 4th Vyatsky per. 39. Tel.: +74956134509. E-mail: felidis@mail.ru
Pastarnak Andrei Y., chief physician of the City Clinical Hospital No. 13 branch No. 1 of the Health Department. 115088, Russia, Moscow, Sharikopodshipnikovskaya str. 3
Gogichaev Tamerlan Kazbekovich, Chief Physician of the City Clinical Hospital No. 29, Branch No. 2, N.E. Bauman Moscow Department of Health.
105094, Russia, Moscow, Golyanovskaya str. 4A
For citations: Dobrokhotova Yu.E., Trofimov D.Yu., Shchegolev A.I., Burmenskaya O.V., Veselovskaya Yu.S., Mitrofanova Yu.V., Olenev A.S., Pastarnak A.Yu., Gogichaev T.K.
Placental PIBF1 gene mRNA expression during preterm labor.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (7): 62-7. (in Russian)
http://dx.doi.org/10.18565/aig.2017.7.62-7
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