Placental PIBF1 gene mRNA expression during preterm labor

Dobrokhotova Yu.E., Trofimov D.Yu., Shchegolev A.I., Burmenskaya O.V., Veselovskaya Yu.S., Mitrofanova Yu.V., Olenev A.S., Pastarnak A.Yu., Gogichaev T.K.

1Department of Obstetrics and Gynecology, Faculty of General Medicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russia; 2Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow 117997, Ac. Oparina str. 4, Russia; 3Branch Two, City Clinical Hospital Twenty-Four, Moscow Healthcare Department, Moscow, Russia; 4Branch One, City Clinical Hospital Thirteen, Moscow Healthcare Department, Moscow, Russia; 5Branch Two, N.E. Bauman, City Clinical Hospital Twenty-Nine, Moscow Healthcare Department, Moscow, Russia
Objective. To investigate the role of a progesterone-induced blocking factor in the genesis of preterm labor.
Subjects and methods. A comprehensive examination was made in 63 puerperas (34 with preterm labor and 29 with term labor).
Results. There was evidence for the effects of micronized progesterone on placental PIBF1 gene mRNA expression. A direct correlation (correlation coefficient r = 0.66 (95% CI 0.22 to 1.12)) was found between plasma progesterone levels and placental PIBF1 gene mRNA expression. Patients with low PIBF1 gene mRNA expression were ascertained to have histological signs of placental insufficiency.
Conclusion. In the presence of progesterone deficiency, the reduced placental PIBF1 gene mRNA expression could lead to the breakdown of maternal adaptive reactions aimed at maintaining pregnancy and will be one of the factors that initiate preterm labor.

Keywords

preterm labor
micronized progesterone
progesterone
progesterone-induced blocking factor
placental insufficiency

Supplementary Materials

  1. Fig. 1. Correlation between blood progesterone level (nmol / L) and mRNA expression of PIBF1 gene, standard units
  2. Fig. 2. Expression of the mRNA of the PIBF1 gene (unit units) in the placenta in patients in the study groups
  3. Fig. 3. Expression of the mRNA of the PIBF1 gene in the placenta in patients with an abortion in an anamnesis
  4. Рис. 4. Уровень прогестерона в периферической плазме, нмоль/л
  5. Fig. 5. Preparation of placenta patient with preterm labor at 34-35 weeks. Staining with hematoxylin and eosin. Signs of placental insufficiency: the presence of fibrinoid degeneration zones in the field of view with the precipitation of calcium salts, the prevalence of intermediate villi
  6. Fig. 6. Preparation of placenta patient with premature birth at 34-35 weeks. Staining with hematoxylin and eosin. The manifestation of compensatory reactions in placental insufficiency: syncytial kidney, syncytocapillary membrane

References

1. Dobrokhotova Yu.E., Kerchelayeva S.B., Kuznetsova O.V., Burdenko M.V. Premature delivery: analysis of perinatal outcomes. Russian Medical Journal. 2015; 23 (20): 1220-3. (in Russian)

2. March of Dimes Foundation, March of Dimes White Paper on Preterm Births: The Global and Regional Toll. NY, USA: March of Dimes Foundation, White Plains; 2009.

3. Experience and tasks of perinatal centers to reduce perinatal and maternal mortality. Information letter of the Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health and Social Development of the Russian Federation. Moscow; 2009. (in Russian)

4. Makarov O.V., Bakhareva I.V., Kuznetsov P.A., Romanovskaya V.V. Modern approaches to predicting preterm labor. Rossiyskiy vestnik akushera-ginekologa. 2007; 7 (6): 10-5. (in Russian)

5. Report form No32 “Information on medical care for pregnant, parturient women and puerperas” for 2012. Russian Federation. (in Russian)

6. The focus is on pregnancy (conference materials). Consillium medicum. 2014; 16(6): 9-15. (in Russian)

7. Goldenberg R.L., Rouse D.J. The pretention of premature birth. N. Engl. J. Med. 1998; 339(5): 313-20.

8. Aylamazyan E.K., Kulakov V.I., Radzinsky V.E., Saveleva G.M., ed. Obstetrics. National guideline. Moscow: GEOTAR-Media; 2008: 160-4. (in Russian)

9. Gibson C.S., MacLennan A.H., Dekker G.A., Goldwater P.N., Dambrosia J.M., Munroe D.J. et al. Genetic polymorphisms and spontaneous preterm birth. Obstet. Gynecol. 2007; 109(2, Pt 1): 384-91.

10. Muglia L., Katz M. The enigma of spontaneous preterm birth. N. Engl. J. Med. 2010; 362(6): 529-35.

11. Menon R., Torloni M.R., Voltolini C., Torricelli M., Merialdi M., Betrán A.P. et al. Biomarkers of spontaneous preterm birth: an overview of the literature in the last four decades. Reprod. Sci. 2011; 18(11): 1046-70.

12. Guillard C., Zidi I., Marcou C., Menier C., Carosella E.D., P. Moreau P. Role of HLA- G in innate immunity through direct actiyation of NF - kB in natural killer cells. Mol. Immunol. 2008; 45(2): 419-27.

13. Sukhikh G.T., Vanko L.V. Immune mechanisms in the physiology and pathology of pregnancy. Immunologiya. 2005; 9(2): 103-8. (in Russian)

14. Szekeres-Bartho J., Polgar B. PIBF: the double edged sword. Pregnancy and tumor. Am. J. Reprod. Immunol. 2010; 64(2): 77-86.

15. Serov VN, Sidelnikova VM, Zharov EV Premature birth. Moscow: Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health and Social Development of the Russian Federation; 2008. (in Russian)

16. Niswander K., Evans A., ed. Obstetrics. Reference book of the University of California. Trans. from English. Moscow; 1999. 571p. (in Russian)

17. Abramchenko V.V. Active management of childbirth. St. Petersburg: SpetsLit; 2003. 664p. (in Russian)

18. Hudić I., Fatusić Z. Progesterone - induced blocking factor (PIBF) and Th(1)/Th(2) cytokine in women with threatened spontaneous abortion. J. Perinat. Med. 2009; 37(4): 338-42. doi: 10.1515/JPM.2009.061.

19. Lin L., Huang Y., Yu Y, Yang Y. Expression of progesterone-induced blocking factor in severe preeclampsia and its association with immune tolerance imbalance. Nan Fang Yi Ke Da Xue Xue Bao. 2015; 35(6): 848-51.

20. Dobrokhotova Yu.E., Karnaukhov V.N., Faizullin A.Z., Ozerova R.I., Demura T.A., Mandrykina Zh.A., Rora L.S. The role of progesterone-dependent immunomodulation and the receptivity of the endometrium in miscarriage. Problem reproduktsii. 2009; 5: 59-62. (in Russian)

21. Szekeres-Bartho J., Wilczynski J.R., Basta P., Kalinka J. Role of progesterone and progestin therapy in threatened abortion and preterm labour. Front. Biosci. 2008; 13: 1981-90.

22. Szekeres-Bartho J. Progesterone receptor-mediated immunomodulation and anti-abortive effects: The role of PIBF. Gynecol. Endocrinol. 2001; 15(Suppl. 5): 43-7. (Proceedings IX World Congress of Gynecological Endocrinology. Hong Kong, December 2–5, 2001.)

Received 16.12.2016

Accepted 23.12.2016

About the Authors

Dobrokhotova Yulia E., MD, Head of the Department of Obstetrics and Gynecology of the Medical Faculty of N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanova str. 1. Tel.: +74952374033. E-mail: pr.dobrohotova@mail.ru
Trofimov Dmitry Y., Doctor of Biological Sciences, Head of the Laboratory of Molecular Genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: d.trofimov@dna-tech.ru
Schegolev Alexander I., MD, Professor, Head of the 2nd Pathoanatomical Department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382892. E-mail: ashegolev@oparina4.ru
Burmenskaya Olga V., Doctor of Biological Sciences, Researcher of the Laboratory of Molecular Genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: o_bourmenskaya@oparina4.ru
Veselovskaya Yulia S., post-graduate student of the Department of Obstetrics and Gynecology of the Faculty of Medicine, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanova str. 1. E-mail: isveselovskaia@gmail.com
Mitrofanova Yulia V., post-graduate student of the Department of Obstetrics and Gynecology of the Medical Faculty, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ostrovityanova str. 1
Olenev Anton S., Candidate of Medical Science, Deputy chief doctor of the City Clinical Hospital No. 24, the head of the Perinatal Center branch.
127287, Russia, Moscow, 4th Vyatsky per. 39. Tel.: +74956134509. E-mail: felidis@mail.ru
Pastarnak Andrei Y., chief physician of the City Clinical Hospital No. 13 branch No. 1 of the Health Department. 115088, Russia, Moscow, Sharikopodshipnikovskaya str. 3
Gogichaev Tamerlan Kazbekovich, Chief Physician of the City Clinical Hospital No. 29, Branch No. 2, N.E. Bauman Moscow Department of Health.
105094, Russia, Moscow, Golyanovskaya str. 4A

For citations: Dobrokhotova Yu.E., Trofimov D.Yu., Shchegolev A.I., Burmenskaya O.V., Veselovskaya Yu.S., Mitrofanova Yu.V., Olenev A.S., Pastarnak A.Yu., Gogichaev T.K.
Placental PIBF1 gene mRNA expression during preterm labor.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (7): 62-7. (in Russian)
http://dx.doi.org/10.18565/aig.2017.7.62-7

Similar Articles

By continuing to use our site, you consent to the processing of cookies that ensure the proper functioning of the site.