Assessment of outcomes of embryo cryotransfer depending on the transcriptional profile of endometrial genes during the implantation window
Objective. To assess the outcomes of assisted reproductive technologies (ART) depending on molecular genetic profiles of differentially expressed endometrial genes.Miroshkina М.I., Korneeva I.Е., Burmenskaya О.V., Мishina N.D.
Materials and methods. The study included 104 patients with tubal and peritoneal factor infertility who were performed pippelle endometrial biopsy in the natural cycle during the implantation window before embryo transfer. Histological and molecular genetic analysis of genes in endometrial samples was conducted using RT-PCR method. Outcomes of ART programs were analyzed after frozen-thawed embryo transfer into the uterine cavity.
Results. The revealed association between the outcomes of ART programs and endometrial expression profile made it possible to determine additional intermediate stages of secretory endometrial development.
Conclusion. The study of expression profiles of mRNA genes LIF, GPX3, AQP3, NDRG1, GNLY, IMPA2, PAEP, IGFBP1, HABP2, DPP4, TAGLN, IL15, POSTN, HLA-DOB, MSX1 allows for identifying the most favorable period for embryo cryotransfer.
Keywords
implantation window
endometrial receptivity
mRNA gene expression
frozen-thawed embryo transfer
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Received 14.01.2020
Accepted 07.02.2020
About the Authors
Maria I. Miroshkina, Postgraduate student of SEC for ART named after F. Paulsen, FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation, Russia. Phone: +7(985)430-38-33. E-mail: maria_mir18@mail.ru4 Oparin str, 117997, Moscow, Russia
Irina E. Korneeva, PhD, Docent, Head of SEC for ART named after F. Paulsen, FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology
Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation, Phone: +7(495) 438-26-22. E-mail: i_korneeva@oparina4.ru
4 Oparin str, 117997, Moscow, Russia
Olga V. Burmenskaya, SD, Head of the Laboratory of Oncological Genetics, FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology
Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation, Phone: +7 (495) 438-22-92. E-mail: o_bourmenskaya@oparina4.ru
4 Oparin str, 117997, Moscow, Russia
Natalia D. Mishina, junior researcher, Laboratory of genomic data analysis FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named
after Academician V.I. Kulakov» Ministry of Healthcare of the Russian Federation. Phone: +7(985)217-29-89. E-mail: mis7ha@gmail.com
4 Oparin str, 117997, Moscow, Russia
For citation: Miroshkina М.I., Korneeva I.Е., Burmenskaya О.V., Мishina N.D. Assessment of outcomes of embryo cryotransfer depending on the transcriptional profile of endometrial genes during the implantation window.
Akusherstvo i Ginekologiya/Obstetrics and gynecology. 2020; 4: 133-139. (In Russian).
https://dx.doi.org/10.18565/aig.2020.4.133-139
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