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Obstetrics and Gynegology2021№5
Non-invasive testing of human preimplantation...

Non-invasive testing of human preimplantation embryos in vitro as a way to predict the outcomes of in vitro fertilization programs

DOI
https://dx.doi.org/10.18565/aig.2021.5.5-16

Valiakhmetova E.Z., Kulakova E.V., Skibina Yu.S., Gryaznov A.Yu., Sysoeva A.P., Makarova N.P., Kalinina E.A

1) Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia; 2) Research and Production Enterprise “Nanostructured Glass Technology" and International Research and Education Center “Structure-Mediated al Nanobiophotonics” Saratov, Russia
The data available in the current literature on non-invasive methods for diagnosing the quality of an embryo and its genetic status were systematically analyzed. The review includes data from foreign and Russian articles published in Pubmed on this topic over the past 3 years. The improvement in the outcome of the IVF program is determined by many factors, including the quality of an embryo. The expansion of knowledge in relation to its development and physiology largely due to various current data processing methods and technologies can determine not only the level of embryonic morphological development, but also to predict the potential for further development. The non-invasiveness, safety, and efficiency of the method are the main criteria for current diagnosis of the potential of the embryo. Numerous studies of the embryo culture medium meet these requirements and seem promising in the selection of high-quality embryos.
Conclusion. The study of the molecular composition of culture media for the embryo makes it possible to comprehensively consider its life cycle, to assess the relationship of cellular metabolism to deep regulatory mechanisms. The development of omix technologies could gain insights into the molecular profile of the embryo culture medium, by identifying and characterizing the biomarkers that are potentially important for the onset of pregnancy. Further improvement of methods for analyzing culture media, processing the data, and increasing the future scope of research can provide a new, non-invasive predictor for the quality of the embryo and its implantation potential.

Keywords

assisted reproductive technologies
culture medium
embryo quality
embryonic metabolic activity
non-invasive pre-implantation genetic testing
in vitro fertilization
Full text (in Russian)

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Received 18.12.2020

Accepted 29.04.2021

About the Authors

Elvira Z. Valiakhmetova, postgraduate student at the Department of Assisted Reproductive Technologies in the Treatment of Infertility, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation. E-mail: ibraeva1988@list.ru.
117997, Russia, Moscow, Ac. Oparina str., 4.
Elena V. Kulakova, PhD, Senior Researcher at the Department of Assisted Reproductive Technologies in the Treatment of Infertility, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation. E-mail: e_kulakova@oparina4.ru.
117997, Russia, Moscow, Ac. Oparina str., 4.
Julia S. Skibina, Ph.D. in physics and mathematics, Director of LLC SPE “Nanostructed Glass Technology” and Deputy Director of International Research and Education Center “Structural Nanobiophotonics”. E-mail: director@nano-glass.ru. 410033, Russia, Saratov, pr. 50 let Octyabrya, 101.
Aleksey Yu. Gryaznov, Researcher of LLC SPE “Nanostructed Glass Technology” and International Research and Education Center “Structural Nanobiophotonics”.
E-mail: director@nano-glass.ru. 410033, Russia, Saratov, pr. 50 let Octyabrya, 101.
Natalya P. Makarova, Dr. Bio. Sci., Leading Researcher at the Department of Assisted Reproductive Technologies in the Treatment of Infertility, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation. E-mail: np_makarova@oparina4.ru.
117997, Russia, Moscow, Ac. Oparina str., 4.
Anastasia P. Sysoeva, embryologist at the Department of Assisted Reproductive Technologies in the Treatment of Infertility, Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation. E-mail: a_sysoeva@oparina4.ru.
117997, Russia, Moscow, Ac. Oparina str., 4.
Elena A. Kalinina, Dr. Med. Sci., Professor, Head of the Department of Assisted Reproductive Technologies in the Treatment of Infertility, Academician V.I. Kulakov
National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation. E-mail: e_kalinina@oparina4.ru.
117997, Russia, Moscow, Ac. Oparina str., 4

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