Mitochondrial dysfunction and embryo quality: prospects for antioxidant correction containing myo-inositol and D-chiroinositol in a 5:1 ratio, manganese and folic acid
Ob’edkova K.V., Ryzhov Ju.R., Khalenko V.V., Ishchuk M.A., Sagurova Ya.M., Lesik E.A., Komarova E.M., Gzgzyan A.M., Bespalova O.N., Kogan I.Yu., Tapilskaya N.I.
Objective: To evaluate the functional activity of mitochondria of cumulus cells and embryo quality in protocols of assisted reproductive technologies (ART) in reproductive-aged patients with anovulatory infertility and obesity taking myo-inositol (MI) and D-chiroinositol (DCI) complex in a 5:1 ratio in combination with manganese and folic acid.
Materials and methods: The study included 22 patients with obesity and anovulatory infertility. The patients of the main group (n=11) received one sachet of Dikirogen complex two times/day for six months before the ART protocol, the comparison group (n=11) did not receive Dikirogen. The obtained embryos were cultured in the EmbryoVisor incubator with time-lapse function. Confocal laser scanning microscopy was used to study the mitochondrial activity of cumulus cells.
Results: No statistically significant differences were found in the comparison of morphokinetic parameters of embryos, but the blastulation rate in the main group was significantly higher than in the comparison group (57.01±1.99 vs 48.19±3.13, p=0.04). The evaluation of mitochondrial networks in cumulus cells showed a significantly higher emission intensity in the main group than in the comparison group (48.28±1.14 and 42.03±1.24, p=0.002).
Conclusion: The combined therapy with antioxidants, vitamins and trace elements (2000 mg MI, 400 mg DHI, 400 μg folic acid, 10 mg manganese) administered for six months before the initiation of the ART protocol can reduce mitochondrial dysfunction of cumulus cells, decrease oxidative stress and lead to the improvement of oocyte quality.
Authors’ contributions: Ob’edkova K.V. – preparing the publication, analysis of the obtained data; Ryzhov Ju.R. – preparing the publication; Khalenko V.V. – statistical processing of the data, confocal microscopy; Ishchuk M.A., Sagurova Ya.M., Lesik E.A. – conducting the embryological stage of the ART protocols; Komarova E.M.: conducting the embryologic stage of ART protocols, developing the study design, preparing the publication; Gzgzyan A.M., Bespalova O.N., Kogan I.Yu. – developing the study design; Tapilskaya N.I. – developing the study design, preparing the publication, analysis of the obtained data.
Conflicts of interest: The authors declare that there are no conflicts of interest.
Funding: The study was supported by the Exploratory scientific research “Creation of innovative approaches in the field of assisted human reproductive technologies using biobanking and import-substituting genetic, regenerative and embryological platforms” No. 1024062500021-3-3.2.2 (FGWN-2024-0020).
Ethical Approval: The study was approved by the Ethical Review Board of D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction, St. Petersburg, Russia dated 12.05.2023 and was performed in accordance with the principles of the Declaration of Helsinki (1964).
Patient Consent for Publication: All patients participating in the study provided an informed consent for the publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Ob’edkova K.V., Ryzhov Ju.R., Khalenko V.V., Ishchuk M.A., Sagurova Ya.M., Lesik E.A., Komarova E.M., Gzgzyan A.M., Bespalova O.N., Kogan I.Yu., Tapilskaya N.I. Mitochondrial dysfunction and embryo quality: prospects for antioxidant correction containing myo-inositol and D-chiroinositol in a 5:1 ratio, manganese and folic acid.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2024; (12): 148-156 (in Russian)
https://dx.doi.org/10.18565/aig.2024.299
Keywords
in vitro fertilization
assisted reproductive technology
mitochondrial dysfunction
time-lapse
inositol
manganese
Dikirogen
embryo
antioxidants
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Received 21.11.2024
Accepted 11.12.2024
About the Authors
Ksenia V. Ob’edkova, PhD, Senior Researcher at the Department of Reproductology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0002-2056-7907
Julian R. Ryzhov, Junior Researcher at the Department of Reproductology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, julian.ryzhov@gmail.com, https://orcid.org/0000-0002-5073-8279
Vladislava V. Khalenko, Junior Researcher at the Department of Reproductology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51.
Mariia A. Ishchuk, Embryologist at the Assisted Reproductive Technology Department, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0002-4443-4287
Yanina M. Sagurova, Embryologist at the Assisted Reproductive Technology Department, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0003-4947-8171
Elena A. Lesik, PhD, Senior Embryologist at the Assisted Reproductive Technology Department, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0003-1611-6318
Evgenia M. Komarova, PhD, Head of the Early Embryogenesis Laboratory of the Department of Reproductology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0002-9988-9879
Alexander M. Gzgzyan, Dr. Med. Sci., Professor, Leading Researcher at the Department of Reproductology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0003-3917-9493
Olesya N. Bespalova, Dr. Med. Sci., Deputy Director for Scientific Work, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia,
Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0002-6542-5953
Igor Yu. Kogan, Dr. Med. Sci., Professor, Corresponding Member of Russian Academy of Sciences, Director, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0002-7351-6900
Natalia I. Tapilskaya, Dr. Med. Sci., Professor, Head of the Department of Reproductology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, Saint-Petersburg, Mendeleevskaya line, 3, +7(812)679-55-51, https://orcid.org/0000-0001-5309-0087
Corresponding author: Julian R. Ryzhov, julian.ryzhov@gmail.com
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