Mitochondrial proteins of peripheral plasma microvesicles as biomarkers of early pregnancy losses
The paper presents the results of a pilot study on the determination of proinflammatory factors of mitochondrial damage-associated molecular patterns (mtDAMP) in pregnant women with physiological pregnancy and recurrent early miscarriages.Bulatova Yu.S., Tetruashvili N.K., Vishnyakova P.A., Vysokikh M.Yu., Marei M.V., Pyataeva S.V., Sukhikh G.T.
Objective. To determine the levels of proinflammatory factors of mtDAMP in the peripheral blood of patients with threatened and recurrent miscarriage versus the similar indicators in women with physiological pregnancy.
Subjects and methods. The investigation enrolled 24 pregnant women whose peripheral plasma samples were selected retrospectively after successful achievement of 22 weeks’ gestation in 12 patients (a control group) and in 12 patients with recurrent early miscarriage (a study group). To determine the level of mtDAMP proteins in the patients of the examined groups, their venous blood was fractionated, microvesicles were isolated from the obtained plasma, and mitochondrial protein levels were analyzed by Western-blotting. The findings were statistically processed using the SPSS program according to the ANOVA method.
Results. Comparative analysis of the relative content of mitochondrial proteins in the plasma microvesicles showed that in the women with threatened and recurrent miscarriage, whose pregnancy spontaneously aborted, the levels of all marker proteins were significantly lower than those in the women of the control group (p < 0.01).
Conclusion. The low levels of proinflammatory factors of peripheral plasma mtDAMP are shown to be an unfavorable factor for early pregnancy.
Keywords
recurrent miscarriage
pregnancy
mitochondria
proinflammatory factors
mitochondrial proteins
inflammation
Supplementary Materials
- Fig. 1. Typical electrophoretogram and Western blot of microvesicles of plasma of peripheral blood of women of the studied groups. Coloring with monoclonal antibodies to porin protein (VDAC1). M - a marker of molecular weight, the arrow indicates the position of the marker 34kDa, H - the norm, B - the habitual miscarriage
- Fig. 2. Level of relative expression of mitochondrial proteins in blood plasma microvysicles. White - the norm, black - miscarriage. A - Porin (VDAC1), B - mtDNA translation initiation protein (TFAM1), B - subunit 1 mitochondrial cytochrome c oxidase (MTCO1). The data are presented as the mean of three measurements ± the standard error of the mean, n = 12 for each group
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Received 03.11.2016
Accepted 11.11.2016
About the Authors
Bulatova Yulia S., Graduate student, Department of Pregnancy Loss Prevention and Therapy, Research Center of Obstetrics, Gynecology, and Perinatology Ministryof Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381477. E-mail: yu.bulatova@mail.ru
Тetruashvili Nana K., Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy, Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381477. E-mail: tetrauly@mail.ru.
Vishnyakova P.A., researcher at the Laboratory of mitochondrial medicine, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: vpa2002@mail.ru
Vysokikh Mikhail Yu., PhD, the head of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387633, ext. 1472. E-mail: m_vysokikh@oparina4.ru
Marey M.V., researcher at the Laboratory of mitochondrial medicine, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: m_marey@oparina4.ru
Pyataeva S.V., PhD. researcher at mitochondrial medicine research group, Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: biosonya@gmail.com
Sukhikh Gennadiy Tikhonovich, Academician of RAS, MD, PhD, Professor, Director, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. Tel.: +74954381800. E-mail: g_sukhikh@oparina4.ru
For citations: Bulatova Yu.S., Tetruashvili N.K.,
Vishnyakova P.A., Vysokikh M.Yu., Marei M.V., Pyataeva S.V., Sukhikh G.T. Mitochondrial proteins of peripheral plasma microvesicles as biomarkers of early pregnancy losses.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (7): 46-51. (in Russian)
http://dx.doi.org/10.18565/aig.2017.7.46-51
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