Clinical and laboratory assessment of the state of placental mitochondria in severe preeclampsia
Objective. To investigate clinico-anamnestic and comprehensive studies of the placental mitochondrial apparatus in early- and late-onset severe preelampsia (PE). Subjects and methods. The investigation enrolled 90 women aged 18-43 years, including 30 with early-onset severe preeclampsia (Group 1), 30 with late-onset severe preeclampsia (Group 2) and 30 somatically healthy women with uncomplicated pregnancy (Group 3). The investigators carried out a comparative analysis of the clinical and anamnestic characteristics of the patients, as well as the relative expression levels of the proteins VDAC1, MT-ND5, and АТР8 in placental tissue by Western blotting. Results. Medical histories in Group 1 women more frequently showed antenatal fetal death (23%) and preeclampsia (40%) than in Group 2 (3 and 13%, respectively; p<0.05). In Group 2, hypertension and proteinuria occurred much later at 33.43±6.48 and 36.83±1.37 weeks’ gestation, respectively. The delivery time was 31.54±3.15, 37.95±1.23, and 39.25±0.72 weeks in Groups 1, 2, and 3, respectively. In Group 1, babies were born preterm in 100% of cases. Analysis of the expression of mitochondrial proteins revealed a significant increase in the level of MT-ND5, the subunit of respiratory chain NADH dehydrogenase ((Complex I) in a group of women with early-onset PE. The study and control groups showed no difference in the expression of VDAC1 and ATP8. Conclusion. The findings suggest that placental mitochondria contribute to the development of oxidative stress that is more pronounced in early-onset preeclampsia.Khodzhaeva Z.S., Vavina O.V., Vishnyakova P.A., Muminova K.T., Tarasova N.V., Vysokikh M.Yu., Sukhikh G.T.
Keywords
preeclampsia
mitochondrial respiration
oxidative stress
Supplementary Materials
- Table. Clinical and anamnestic characteristics of the examined women (data is presented as the mean ± standard error of the mean)
- Fig. 1. The level of relative expression of VDAC1, MT-ND5 and ATP8 in placental tissue in normal pregnancy and pregnancies with PE according to Western blot analysis
- Fig. 2. Level of relative expression of tumor suppressor proteins p53 (A), Bcl2 (B) and Bax (B) in placental tissue, analyzed by Western blot analysis. * p<0.05
- Fig. 3. Autophagy and quality control system for mitochondria in fibroblasts obtained from the placenta from normal and preeclamptic pregnancies
References
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Received 26.04.2017
Accepted 28.04.2017
About the Authors
Zulfiya Sagdullaevna Khodzhaeva, M.D., Ph.D., Professor, Head of Maternal-Fetal Medicine Department, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117437, Russia, Moscow, Ac. Oparina str. 4. E-mail: z_khodzhaeva@oparina4.ruOlga Vladimirovna Vavina, doctor оf Labor Department, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117437, Russia, Moscow, Ac. Oparina str. 4. E-mail: o_vavina@oparina4.ru
Polina Alexandrovna Vishnyakova, researcher at the Laboratory of mitochondrial medicine, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117437, Russia, Moscow, Ac. Oparina str. 4. E-mail: vpa2002@mail.ru
Kamilla Timurovna Muminova, junior research fellow of the 1st obstetric Department of pathology of pregnancy, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117437, Russia, Moscow, Ac. Oparina str. 4. E-mail: kamika91@mail.ru
Nadezhda Valeryevna Tarasova, PhD, researcher at the Laboratory of mitochondrial medicine, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117437, Russia, Moscow, Ac. Oparina str. 4. E-mail:n_tarasova@oparina4.ru
Mikhail Yuryevich Vysokikh, PhD, Head of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117437, Russia, Moscow, Ac. Oparina str. 4. E-mail: m_vysokikh@oparina4.ru
Sukhikh Gennady T., MD, PhD, Academician of Russian Academy of Sciences, Professor, Director of Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381800. E-mail: gtsukhikh@mail.ru
For citations: Khodzhaeva Z.S., Vavina O.V., Vishnyakova P.A., Muminova K.T.,
Tarasova N.V., Vysokikh M.Yu., Sukhikh G.T. Clinical and laboratory assessment
of the state of placental mitochondria in severe preeclampsia.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (9): 72-6. (in Russian)
http://dx.doi.org/10.18565/aig.2017.9.72-6
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