Immunoregulatory and transport proteins as markers of antenatal prognosis of preterm infants’ status in women with preterm prelabor rupture of membranes
Aim. To investigate serum levels of lactoferrin (LF), alpha-2-macroglobulin (a2-MG), alpha-1-antitrypsin (a1-AT), and albumin and their association with neonatal outcomes in pregnant women with preterm prelabor rupture of membranes (PROM). Materials and methods. This study tested serum samples from women with PROM at 24–33 weeks’ gestation, who gave birth to preterm infants without signs of intrauterine infection (IUI) (n = 35), mild IUI (n = 30) and severe IUI (n = 28). A control group comprised 27 healthy women who were tested at 24–33 weeks’ gestation. Serum samples were tested for LF concentration using ELISA; a2-MG, a1-AT were determined by a quantitative rocket immunoelectrophoresis assay and albumin by the biochemical method. Results. Women with PROM, regardless of newborns’ health status, had statistically significantly higher serum levels of LF and lower levels of albumin than women in the control group. Serum levels of a2-MG and a1-AT in pregnant women with PROM, who gave birth to babies with no signs of IUI, did not differ from those of healthy pregnant women. But they were significantly higher in mothers of neonates with mild forms of IUI and, on the contrary, significantly lower in mothers of neonates with severe IUI. The discriminant analysis showed that in a pregnant woman with PROM, the most sensitive prognostic marker for IUI in the fetus and newborn was the serum level of a2-MG. Serum levels of a2-MG ranging from 2.2 to 3.0 g/l were 86% predictive of the birth of a child without IUI; a2-MG level>3.0 was 85% predictive of the birth of a child with a moderate IUI; a2-MG level <2.2 g/l was 86% predictive severe generalized forms of IUI in a newborn. Conclusion. Testing for serum levels of LF, a2-MG, a1-AT, and albumin in pregnant women with PROM can be used to predict their babies’ health status in the early neonatal period.Grigor’eva E.Yu., Renge L.V., Zorina V.N., Bazhenova L.G., Likhacheva V.V.
Keywords
premature rupture of membranes
intrauterine infection
lactoferrin
alpha-2-macroglobulin
alpha-1-antitrypsin
albumen
References
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Received 14.10.2019
Accepted 29.11.2019
About the Authors
Ekaterina Yu. Grigoryeva, assistant of the Department of Obstetrics and Gynecology of NSIFTPh – Branch Campus of the FSBEI FPE RMACPE MOH.Phone: +7(3843)454-873. E-mail: l.renge@mail.ru; ORCID:0000-0002-8623-729Х
654005, Russia, Novokuznetsk, Stroiteley, 5.
Lyudmila V. Renge, MD, Professor of the Department of Obstetrics and Gynecology of NSIFTPh – Branch Campus of the FSBEI FPE. RMACPE MOH.
Phone:+7(3843)454-873. E-mail: l.renge@mail.ru; ORCID:0000-0002-7237-9721
654005 Russia, Novokuznetsk, Stroiteley, 5.
Veronika N. Zorina, D .Sc., Deputy Director for Science, Institute of Highly Pure Biopreparations. ORCID:0000-0001-9183-7663
197110, Russia, Saint Petersburg, Pudozhskaya, 7.
Lyudmila G. Bazhenova,MD, Professor, Head of the Department of obstetrics and gynecology of ofNSIFTPh – Branch Campus of the FSBEI FPE RMACPE MOH.
Тел.: +7(3843)454-873 E-mail: l_bagenova@mail.ruORCID:0000-0002-3399-8028
654005; Russia, Novokuznetsk, Stroiteley, 5.
VictoriaV. Lihacheva, PhD, Associate Professorof the Department of Obstetrics and Gynecology of NSIFTPh – Branch Campus of the FSBEI FPE RMACPE MOH.
Phone: +7(3843)454-873E-mail: postmaster@ngiuv.ru; ORCID: 0000-0002-5637-7590
654005,Russia, Novokuznetsk, Stroiteley, 5.
For citation: Grigor’eva E.Yu., Renge L.V., Bazhenova L.G., Zorina V.N., Likhacheva V.V. Immunoregulatory and transport proteins as markers of antenatal prognosis of preterm infants’ status in women with preterm prelabor rupture of membranes.
Akusherstvo i Ginekologiya/ Obstetrics and gynecology. 2020;3: 148-54. (In Russian).
https://dx.doi.org/10.18565/aig.2020.3.148-154
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