Efficiency of maturation of oocytes obtained from cancer patients during ovariectomy
Kovalskaya E.V., Kirillova A.O., Bunyaeva E.S., Khabas G.N., Kamaletdinov N.S., Nazarenko T.A., Abubakirov A.N.
Objective. To study in vitro maturation of oocytes obtained from cancer patients during ovariectomy.
Subjects and methods. The investigation enrolled 24 patients aged 16 to 42 years who received cancer treatment. During surgery, partial or complete ovariectomy was performed, oocyte-cumulus complexes were removed from the ovarian medulla after ovariectomy, the obtained cells were matured, the oocytes were fertilized, and embryos were cultured. The rates of oocyte maturation, fertilization, and fragmentation and blastocyst formation were estimated.
Results. A total of 287 oocyte-cumulus complexes were obtained. The rates of oocyte maturation, fertilization, and fragmentation were 41.4, 20.0, and 100%, respectively; the rate of blastocyst formation was 27.3%. Genetic analysis of the trophectoderm of blastocysts revealed that the latter contained a euploid chromosome set.
Conclusion. Despite the fact that the rate of in vitro maturation of oocytes obtained from the ovarian medulla is low, the method is promising for preserving the reproductive potential in cancer patients.
Keywords
fertility preservation
oocyte maturation
oncology
ovariectomy
assisted reproductive technologies
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Received 08.04.2019
Accepted 19.04.2019
About the Authors
Evgeniya V. Kovalskaya, embryologist, 1st Department of Gynecology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia named after Academician V.I. Kulakov. Tel.: +79263072710. E-mail: kovalskaya.evgeniya@gmail.com. 117997, Russia, Moscow, Ac. Oparina str. 4.Anastasia O. Kirillova, PhD, embryologist, 1st Department of Gynecology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia named after Academician V.I. Kulakov. Tel.: +79267815524. E-mail: stasia.kozyreva@gmail.com. 117997, Russia, Moscow, Ac. Oparina str. 4.
Ekaterina S. Bunyaeva, post-graduate student, 1st Department of Gynecology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia named after Academician V.I. Kulakov. Tel.: +74954382622. E-mail: es_bunyaeva@mail.ru. 117997, Russia, Moscow, Ac. Oparina str. 4.
Grigory N. Khabas, Ph.D., head of the Department of Innovative Oncology and Gynecology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia named after Academician V.I. Kulakov. E-mail: khabas@list.ru. 117997, Russia, Moscow, Ac. Oparina str. 4.
Nail S., Kamaletdinov embryologist, 1st Department of Gynecology, National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health
of Russia named after Academician V.I. Kulakov. E-mail: sunsh86@mail.ru. 117997, Russia, Moscow, Ac. Oparina str. 4.
Tatyana A. Nazarenko, Doctor of Medical Sciences, Professor, head of Institute of Reproductive Medicine, National Medical Research Center for Obstetrics,
Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. E-mail: t.nazarenko@mail.ru
117997, Russia, Moscow, Ac. Oparina str. 4.
Aydar N. Abubakirov, PhD, head of the 1st Department of Gynecology, National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia. Тel.: +7(495)4382622. Е-mail: nondoc555@yahoo.com
117997, Russia, Moscow, Ac. Oparina str. 4.
For citation: Kovalskaya E.V., Kirillova A.O., Bunyaeva E.S., Khabas G.N., Kamaletdinov N.S., Nazarenko T.A., Abubakirov A.N.
Efficiency of maturation of oocytes obtained from cancer patients during ovariectomy.
Akusherstvo i Ginekologiya/ Obstetrics and gynecology. 2019; 9: 87-91.(In Russian)
https://dx.doi.org/10.18565/aig.2019.9.87-91
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