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The Journal "Obstetrics and Gynecology"Journal HistoryAims and ScopePolicies on Conflict of Interest, Human and Animal Rights, and Informed ConsentEditorial ProcessData sharing statementAdvertising PolicyThe Process for Handling Cases Requiring Corrections, Retractions, and Editorial Expressions of ConcernEditorial BoardEditorial CounsilInformation for ProfessionalsNewsContacts
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For authors
InstructionsInformed consent policyContract offerEASE GuidelinesICJME CriteriaWAME Recommendations on ChatGPT and Chatbots in Relation to Scholarly Publications
Reviewing
Procedure for reviewingReviewers 2023-2024
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Obstetrics and Gynegology2025№11

Use of complex of natural antimicrobial peptides and cytokines for the treatment of patients with abnormal cervical screening results “atypical squamous cells of uncertain significance”

DOI
https://dx.doi.org/10.18565/aig.2025.331

Dikke G.B., Sukhanov A.A., Kukarskaya I.I., Shilova N.V.

1) F.I. Inozemtsev Academy of Medical Education, Saint Petersburg, Russia; 2) Tyumen Perinatal Center, Tyumen, Russia; 3) Tyumen State Medical University, Ministry of Health of the Russian Federation, Tyumen, Russia; 4) National Research Nuclear University MEPhI, Moscow, Russia

Objective: To evaluate the frequency of cervical lesions with atypical squamous cells of uncertain significance (ASCUS) in patients with chronic endometritis (CE) and the effectiveness of treatment using a complex of natural antimicrobial peptides and cytokines.
Materials and methods: A prospective open-label randomized controlled trial included 1,126 patients with CE. Group I (n=563) received a natural antimicrobial peptide and cytokine complex (Superlymph) at a dose of 25 U/day per vaginam for 20 days. Group II (n=563) did not receive the peptide-cytokine complex. Both groups underwent antimicrobial therapy. Examination methods included real-time polymerase chain reaction and Pap test.
Results: The patients' age ranged from 18 to 45 years: 36.0 (3.1) years in Group I and 35.9 (4.4) years in Group II (p=0.22). ASCUS was detected in 47.8% (256/563) and 42.6% (240/563) of patients (p=0.37), and human papillomavirus (HPV) was identified in 71.6% (403/563) and 71.8% (404/563), respectively (p=1.0). In patients who suffered from ASCUS before treatment, the condition improved in 85.6% (219/256) and 45.8% (110/240) in groups I and II, respectively. The potential for recovery was 2 times higher when using Superlymph (ОР=1,87, 95% ДИ 1,61; 2,16). HPV-negative status after treatment was observed in 85.4% (481/563) of patients in Group I versus 29.7% (167/563) in Group II (OR=0.21, 95% CI 0.17; 0.26, p<0.001). 
Conclusion: Among patients with CE, the prevalence of ASCUS is 45.2%, and the prevalence of HPV-positive status is observed in 71.7% of women. Combination therapy with Superlymph promotes the resolution of ASCUS in 85.6% of patients (a 2-fold risk reduction compared to antibacterial/antimycotic therapy alone) with a negative HPV test rate in 85.4% of CE patients after treatment.

Authors' contribution: Dikke G.B. – study concept and design, analysis of the statistical processing of clinical material and its interpretation, data search, text composition and editing; Sukhanov A.A. – collection of clinical material, electronic database arrangement, text composition; Kukarskaya I.I. – clinical study site management, study supervision; Shilova N.V. – statistical analysis of the study results, text composition.
Conflicts of interest: The authors report no conflicts of interest and guarantee the original nature of the study.
Funding: The study had no financial support. Publication of the article was sponsored by OOO Biotechpharm.
Ethical Approval: The study was approved by the local Ethics Committee (final protocol version v3.0 dated July 20, 2019).
Patient Consent for Publication: The patients signed informed consent for the publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Dikke G.B., Sukhanov A.A., Kukarskaya I.I., Shilova N.V. Use of complex of 
natural antimicrobial peptides and cytokines for the treatment of patients with 
abnormal cervical screening results “atypical squamous cells of uncertain significance”.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2025; (11): 130-140 (in Russian)
https://dx.doi.org/10.18565/aig.2025.331

Keywords

chronic endometritis
ASCUS
cervical cancer
complex of natural antimicrobial peptides and cytokines
Superlymph
Full text (in Russian)

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Received 14.11.2025

Accepted 27.11.2025

About the Authors

Galina B. Dikke, Dr. Med. Sci., Professor, Department of Obstetrics and Gynecology with a Course of Reproductive Medicine, F.I. Inozemtsev Academy of Medical Education, 22 Liter M, Moskovskiy Ave., St. Petersburg, 190013, Russia, galadikke@yandex.ru, https://orcid.org/0000-0001-9524-8962
Anton A. Sukhanov, PhD, Head of the Department of Family Planning and Reproduction, Tyumen Perinatal Center, 1 Daudelnaya str., Tyumen, 625002, Russia; Associate Professor, Department of Obstetrics and Gynecology, Tyumen State Medical University, Ministry of Health of Russia, 10 Permyakov str., Tyumen, 625013, Russia, saa2505anton@yandex.ru, https://orcid.org/0000-0001-9092-9136
Irina I. Kukarskaya, Dr. Med. Sci., Professor at the Department of Obstetrics, Gynecology and Reanimatology with a Course of Clinical Laboratory Diagnostics, Tyumen State Medical University, Ministry of Health of Russia, 10 Permyakov str., Tyumen, 625013, Russia; Chief Physician, Tyumen Region Perinatal Center, 1 Daudelnaya str., Tyumen, 625002, Russia; Chief Specialist in Obstetrics and Gynecology, Department of Health of the Tyumen Region, https://orcid.org/0000-0002-8275-3553
Natalya V. Shilova, PhD, Associate Professor, Department of Industrial Pharmacy, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 31 Kashirskoe shosse, Moscow, 115409, Russia, nvshilova@gmail.com, https://orcid.org/0000-0001-6734-0147
Corresponding author: Galina B. Dikke, galadikke@yandex.ru

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