Survival and composition of the cellular component of breast milk under various conditions of its expression and storage
Objective. To determine the composition and survival of the cellular component of breast milk under various conditions of its single and double pumping and storage.Ryumina I.I., Fatkhudinov T.Kh., Arutyunyan I.V., Makarov A.V., Lokhonina A.V., Narogan M.V., Sharipova K.R., Orlovskaya I.V., Zubkov V.V., Baibarina E.N.
Materials and methods. Eighty-six breast milk samples were collected using a Symphony Medela single (n = 63) or double (n = 23) clinical breast pump. Each sample was divided into two halves and stored for 72 hours when it was cooled to 4°C or room temperature. The fractions of living cells were assessed at 4, 24, and 72 hours after breast milk expression. Flow cytofluorometry was used to determine the expression of specific surface and intracellular markers.
Results. The total number of cells in breast milk did not depend on the method of its expression, gestational age, or number of fetuses; the median was about 80,000 cells per ml. The main milk cell populations were epithelial cells and white blood cells; vimentin-positive stromal cells were a minor population. The storage of breast milk, regardless of its expression method, led to a gradual decrease in the number of living cells due to the partial death of short-lived leukocyte subpopulations. After 4 hours, about a third of all cells remained alive; cooling the milk to +4°C could prolong the period of this level of cell survival. Moreover, this effect was more pronounced in the samples obtained using a double clinical breast pump.
Conclusion. The total number of cells in breast milk does not depend on its expression method, gestational age, or number of fetuses. To preserve the cellular component of the expressed breast milk, the latter can be stored at room temperature for the first 4 hours when it was cooled to +4°C for 24 hours; in this case double pumping has some advantage over single pumping.
Keywords
breast milk
cells
storage mode
breast pump
single pumping
double pumping
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Received 13.11.2019
Accepted 29.11.2019
About the Authors
Irina I. Ryumina, MD, Head of the Department of Pathology of Newborns and Premature Children, Scientific and Research Center for Microbiology, AGP named after Academician V.I. Kulakova “Ministry of Health of Russia. Tel.: +7 (903) 7708048. E-mail: i_ryumina@oparina4.ruAddress: 117997, Russia, Moscow, ul. Academician Oparin, d. 4.
Timur Kh. Fatkhudinov, MD, Deputy Director for Scientific Development, Federal State Budget Scientific Institution Scientific Research Institute of Human Morphology, Leading Scientific Researcher at the Laboratory of Regenerative Medicine, Scientific Research Center for Scientific and Applied Mathematics Academician V.I. Kulakova Ministry of Health of Russia. Tel.: +7 (903)256-11-57. E-mail: tfat@yandex.ru. ORCID iD 0000-0002-6498-5764.
Address: 117997, Russia, Moscow, ul. Academician Oparin, d. 4.
Irina V. Arutyunyan, Ph.D., Senior Researcher, Laboratory of Regenerative Medicine, Scientific Research Center of Medical Sciences named after A.P. Academician V.I. Kulakova “Ministry of Health of Russia, NS growth and development laboratories of FSBI NIIMCH. Tel.: +7 (926)147-44-30. E-mail: labrosta@yandex.ru.
ORCID iD 0000-0002-4344-8943.
Address: 117997, Russia, Moscow, ul. Academician Oparin, d. 4.
Andrey V. Makarov, candidate of medical sciences, senior researcher at the Laboratory of Regenerative Medicine of the Scientific and Research Center “AGP named
after Academician V.I. Kulakova “Ministry of Health of Russia. Tel.: +7 (903) 256-34-04. E-mail: anvitmak@yandex.ru. ORCID iD 0000-0003-2133-2293.
Address: 117997, Russia, Moscow, ul. Academician Oparin, d. 4.
Anastasia V. Lokhonina, Researcher at the Laboratory of Regenerative Medicine, Russian Research Center for Medical Research “AGP named after Academician V.I. Kulakova of the Ministry of Health of Russia. Tel.: +7 (910)431-66-76. Email: nastya.serbsky@gmail.com. ORCID ID: 0000-0001-8077-2307.
Address: 117437, Russia, Moscow, ul. Academician Oparin, d. 4.
Marina V. Narogan, MD, Leading Researcher, Department of Pathology of Newborns and Premature Children, NICC “AGP named after Academician V.I. Kulakova
“Ministry of Health of Russia; Professor, Department of Neonatology, Federal State Autonomous Educational Institution of Higher Education “First MGMU named
after THEM. Sechenov »Ministry of Health of Russia (Sechenov University). Tel.: +7 (905) 5501621. E-mail: m_narogan@oparina4.ru
Address: 117997, Russia, Moscow, ul. Academician Oparin, d. 4; 119991, Moscow, st. Trubetskaya, d. 8, p. 2.
Kamila R. Sharipova, Clinical Resident of the Scientific and Research Center “AGP named after Academician V.I. Kulakova “Ministry of Health of Russia.
Tel.: +7 (977) 503-47-21. Email: k.r.sharipovaa@gmail.com
Address: 117997, Russia, Moscow, ul. Academician Oparin, d. 4.
Irina V. Orlovskaya, head of the clinical work of the department of pathology of newborns and premature babies NICC “AGP named after Academician V.I. Kulakova “Ministry of Health of Russia. Tel.: +7 (916) 6962056. E-mail: i_orlovskaya@oparina4.ru.
Address: 117997, Russia, Moscow, ul. Academician Oparin, d. 4.
Victor V. Zubkov, MD, director of the Institute of Neonatology and Pediatrics, NICC “AGP named after Academician V.I. Kulakova “Ministry of Health of Russia; Professor, Department of Neonatology, Federal State Autonomous Educational Institution of Higher Education “First MGMU named after THEM. Sechenov »Ministry of Health of Russia (Sechenov University).
Elena N. Baibarina, Chief Researcher, Institute of Neonatology and Pediatrics, NICC “AGP named after Academician V.I. Kulakova “Ministry of Health of Russia.
For citation: Ryumina I.I., Fatkhudinov T.Kh., Arutyunyan I.V., Makarov A.V., Lokhonina A.V., Narogan M.V., Sharipova K.R., Orlovskaya I.V., Zubkov V.V., Baibarina E.N. Survival and composition of the cellular component of breast milk under various conditions of its expression and storage.
Akusherstvo i Ginekologiya/ Obstetrics and gynecology. 2020; 3: 164-72.(In Russian).
https://dx.doi.org/10.18565/aig.2020.3.164-172
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