Role of mitochondrial DAMPs in the manifestation of hypertensive complications of pregnancy
Objective. To comparatively analyze the level of the mitochondrial proteins TFAM, OPA1, and VDAC1 as possible damage-associated molecular patterns (DAMPs) in the microvesicular fraction from the plasma of pregnant women with preeclampsia (PE), chronic/gestational hypertension and that of healthy pregnant women.Skovorodina T.V., Vishnyakova P.A., Tsvirkun D.V., Shmakov R.G., Vysokikh M.Yu.
Material and methods. The microvesicular fraction was obtained from the plasma of patients by differential centrifugation. The levels of TFAM, OPA1, and VDAC1 were determined by chemiluminescent western blotting.
Results. The severe PE group was found to have a significant increase in the expression level of ОРА1-L and pre-TFAM proteins and a correlation with the level of proteinuria as an important diagnostic marker for PE.
Conclusion. The ОРА1-L and pre-TFAM proteins can lay claim to being PE-associated DAMPs.
It is necessary to perform further investigations of the association between certain DAMPs and the development of PE.
Keywords
preeclampsia
markers
damage-associated molecular patterns
oxidative stress
Supplementary Materials
- Fig . 1. Level of relative expression of OPA 1- L and OPA 1- S (A), pre-TFAM and TFAM (C) in microvesicles from the plasma of women in the control group, the group with severe preeclampsia , with moderate preeclampsia and chronic / gestational hypertension according Western - Blot - analysis. When calculating expression, the protein signal was normalized to the signal of the mitochondrial protein VDAC 1. The data are presented as the mean ± standard error of the mean. * p <0.05
- Fig . 2. Correlation curve, reflecting the dependence of the level of relative expression of pre - TFAM and proteinuria indices. The Pearson correlation coefficient r = 0 , 3 for p = 0 , 009
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Received 09.06.2017
Accepted 23.06.2017
About the Authors
Skovorodina Tatyana Vyacheslavovna, Ph.D., Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia.117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: t_skovorodina@oparina4.ru
Vishnyakova Polina A., researcher at the Laboratory of mitochondrial medicine, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of the Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: vpa2002@mail.ru
Tsvirkun Darya Viktorovna, PhD, Researcher of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology and Perinatology.
117997, Russia, Moscow, Ac. Oparin str. 4. E-mail: darunyat@gmail.com
Shmakov Roman G., chief physician, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow Ac. Oparina str. 4. Tel.: +79255859171. E-mail: r_shmakov@oparina4.ru
Vysokikh Mikhail Yu., PhD, Head of mitochondrial medicine research group, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of the Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: m_vysokikh@oparina4.ru
For citations: Skovorodina T.V., Vishnyakova P.A., Tsvirkun D.V., Shmakov R.G., Vysokikh M.Yu. Role of mitochondrial DAMPs in the manifestation of hypertensive complications of pregnancy. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (12): 84-8. (in Russian)
https://dx.doi.org/10.18565/aig.2017.12.84-88
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