The role of E-cadherin in the formation of intrauterine growth restriction
Objective. To study the relationship between levels of soluble (80-kd) and transmembrane (120-kd) forms of E-сadherin in placentas from pregnancies with intrauterine growth restriction. Materials and methods. The study was done on 38 placentas: 18 from pregnancies with intrauterine growth restriction (main group) and 20 from normal pregnancies (control group). Levels of soluble and transmembrane E-сadherin in placentas were determined by Western blotting. Measurement of E-сadherin gene expression were made by real-time polymerase chain reaction. Results. In the group with intrauterine growth restriction the level of the transmembrane form of E-cadherin was reduced by 2.5 times, and the soluble form by 4.8 times (p <0.05). Their ratio was of 1.6 in the group with intrauterine growth restriction,and of – 0.8 in control group (in both cases p <0.05). The level of expression of the E-cadherin gene CDH1 in the placentas from pregnancies with fetal growth restriction was reduced by 1.3 times as compared to the control group (p> 0.05). Conclusion. The low level of soluble E-cadherin in the placenta leads to a decrease in proliferation and mobility of trophoblast cells, which may be the cause of placental insufficiency and, as a consequence, intrauterine growth restrictionKrasnyi A.M., Khachaturyan A.A., Kan N.E., Khachatryan Z.V., Tyutyunnik V.L., Volgina N.E., Ganichkina M.B., Mantrova D.A., Sadekova A.A.
Keywords
intrauterine growth restriction
soluble E-cadherin
cell adhesion
placenta
References
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Received 01.12.2017
Accepted 23.12.2017
About the Authors
Aleksey M. Krasnyi, PhD, the head of the cytology laboratory, National Medical Research Center of Obstetrics, Gynecology and Perinatology named afterAcademician V.I. Kulakov, Ministry of Health of Russia; senior researcher of evolutionary developmental biology laboratory N.K. Koltzov Institute of Developmental Biology
of Russian Academy of Sciences. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382272. E-mail: alexred@list.ru
Anuta A. Khachaturyan, postgraduate student of the National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79268453953. E-mail: x.anyt37@mail.ru
Natalia E. Kan, PhD, MD, the head of the obstetric department, National Medical Research Center of Obstetrics, Gynecology and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79262208655. E-mail: kan-med@mail.ru. Researcher ID B-2370-2015. ORCID ID 0000-0001-5087-5946
Zarine V. Khachatryan, postgraduate student of the National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79096562456. E-mail: z.v.khachatryan@gmail.com
Victor L. Tyutyunnik, PhD, MD, the head of the obstetric physiological department, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79039695041. E-mail: tioutiounnik@mail.ru. Researcher ID B-2364-2015. ORCID ID 0000-0002-5830-5099
Nadezda E. Volgina, scientific researcher of the cytology laboratory, National Medical Research Center of Obstetrics, Gynecology and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382272. E-mail: n_volgina@oparina4.ru
Maria B. Ganichkina, postgraduate student of the National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79152397743. E-mail: mariaganichkina@yandex.ru
Diana A. Mantrova, postgraduate student of the National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79096367259. E-mail:d-mantrova@yandex.ru
Alsu A. Sadekova, scientific researcher of the cytology laboratory, National Medical Research Center of Obstetrics, Gynecology and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382272. E-mail: a_sadekova@oparina4.ru
For citations: Krasnyi A.M., Khachaturyan A.A., Kan N.E., Khachatryan Z.V., Tyutyunnik V.L., Volgina N.E., Ganichkina M.B., Mantrova D.A., Sadekova A.A. The role of E-kadherin in the formation of intrauterine growth restriction. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (6): 38-43. (in Russian)
https://dx.doi.org/10.18565/aig.2018.6.38-43
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