Prevalence of HPV types in patients with cervical intraepithelial neoplasia of different severity
Objective. To study the prevalence of HPV (human papillomavirus) types in patients with squamous intraepithelial lesions (SIL) of different severity and cervical cancer in comparative perspective.Mzarelua G.M., Nazarova N.M., Nekrasova M.E., Sycheva E.G., Bourmenskaya O.V., Starodubtseva N.L., Asaturova A.V., Habas G.N., Pavlovich S.V.
Subjects and methods. This prospective study included 145 women aged 18 to 49 years. The survey consisted of: collecting complaints, anamnesis, gynecological status, conducting extended colposcopy, molecular biological methods (multiplex PCR with detection results in real-time for quantification and typing of HPV 21 types), cytological examination, histological analysis of biopsy material. 5 groups were formed including 145 women: 31 patients (21,3%) – NILM (I group), 30 patients (20,6%) – chronic cervicitis (histologically confirmed) (group II), 37 patients (22,7%) – LSIL (III group), 31 patients (21,3%) – HSIL (IV group ) and 14 patients (9,6%) – cervical cancer (V group).
Results. The most significant risk factors for cervical intraepithelial lesions are: early sexual initiation, great number of sexual partners (>4), sexual transmitted infections (STI) history. The most frequent HPV types were 16, 31, 33 and 51 types. We demonstrated that HPV type 16 was significantly more prevalent in the group with HSIL and squamous cervical cancer compared to group II (chronic cervicitis) and group III (LSIL). HPV 31 and 33 types are more prevalent in the group with HSIL. According to our results, there is the prevalence of the group A9 HPV in women with LSIL, HSIL and cervical cancer compared to group II (chronic cervicitis).
Conclusion. Our results demonstrate the key role of A9 HPV group in the development of premalignant and malignant cervical disease. It should also be noted that in patients with LSIL in addition to A9 HPV group there is a trend to increase the incidence of HPV groups A7, A10, A5.
Keywords
human papillomavirus
chronic cervicitis
cervical intraepithelial neoplasia
cervical cancer
real time PCR
Supplementary Materials
- Table 1. Risk factors for development of SIL
- Table 2. Correlation analysis of the main risk factors for the development of SIL
- Table 3. Correlation analysis of the main risk factors and the presence of SIL of varying severity
- Table 4. Comparative data of SIL risk factor assessment by groups
- Table 5. Comparative assessment of the occurrence of groups and types of HPV
- Table 6. HPV groups at different degrees of epithelial involvement of the cervix
- Figure. The presence of HPV in different groups
References
1. Социально значимые заболевания населения России в 2014 году (статистические материалы). М.: Министерство здравоохранения Российской Федерации, Департамент мониторинга, анализа и стратегического развития здравоохранения ФГБУ “Центральный научно-исследовательский институт организации и информатизации здравоохранения” Минздрава России; 2015. Available at: https://www.rosminzdrav.ru/ [Socially significant diseases of the population of Russia in 2014 (statistical materials). M .: Ministry of Health of the Russian Federation, Department for Monitoring, Analysis and Strategic Development of Health of the Federal State Institution of Health Care “Central Research Institute of Health Organization and Informatization” of the Ministry of Health of Russia; 2015. Available at: https://www.rosminzdrav.ru/ (in Russian)]
2. Сухих Г.Т., Прилепская В.Н., ред. Профилактика рака шейки матки. Руководство для врачей. 3-е изд. М.: МЕДпресс-информ; 2012.192с. [Sukhikh G.T., Prilepskaya V.N. Cervical cancer prevention: guidelines for physicians. 3rd ed. Moscow: MEDpress-inform, 2012: 192p. (in Russian)]
3. Doorbar J., Egawa N., Griffin H., Kranjec C., Murakami I. Human papillomavirus molecular biology and disease association. Rev. Med. Virol. 2015; 25(Suppl. 1): 2-23.
4. Назарова Н.М., Прилепская В.Н., Сычева Е.Г., Зардиашвили М.Д., Бурменская О.В. Новые возможности в диагностике «малых» форм поражений эпителия шейки матки, ассоциированных с папилломавирусной инфекцией. Гинекология. 2015; 17(5): 32-6. [Nazarova N.M., Prilepskaya V.N., Sycheva E.G. M.D.Zardiashvili, O.V.Bourmenskaya. New features in the diagnosis of “lesser” abnormalities of cervical epithelium associated with HPV infection. Ginekologiya. 2015; 17(5): 32–36. (in Russian)]
5. Prilepskaya V.N., Nazarova N.M., Trofimov D.Y., Bestaeva N.V., Bourmenskaya O.V., Kogan E.A., Sulamanidze L.A. Human papillomavirus infection caused by HPV types 52 and 58 and its role in the development of cervical intraepithelial neoplasia. In: Еurogin 2015. HPV Infection and Related Cancers: Translating Research Innovations into Improved Practice. Sevilla, Spain, 4-7 February 2015): 251.
6. Shakya S., Syversen U., Åsvold B.O., Bofin A.M., Aune G., Nordbø S.A. et al. Prevalence of human papillomavirus infection among women in rural Nepal. Acta Obstet. Gynecol. Scand. 2016; 96(1): 29-38.
7. Li Z.M., Zeng L.Q., Peng X.H., Mao L.Z., Sun X.L., Li Y.H., Luo X.P. Analysis of clinical and pathological characteristics of high- risk HPV-negative carcinoma of the uterine cervix. Zhonghua Fu Chan Ke Za Zhi. 2016; 51(9): 683-7.
8. Siegler E., Sharir K., Lavie O., Saked-Misan P., Machulki L., Auslender R. et al. The prevalence of HPV types in women with CIN 2-3 or cervical cancer in Haifa District, Israel. Minerva Ginecol. 2016; 16(9):109-18.
9. Zeng Z., Yang H., Li Z., He X., Griffith C.C., Chen X. et al. Prevalence and genotype distribution of HPV infection in China: analysis of 51,345 HPV genotyping results from China’s Largest CAP Certified Laboratory. J. Cancer. 2016; 7(9): 1037-43.
10. Piroozmand A., Mostafavi Zadeh S.M., Madani A., Soleimani R., Nedaeinia R., Niakan M. et al. The association of high risk human papillomaviruses in patients with cervical cancer: an evidence based study on patients with squamous cell dysplasia or carcinoma for evaluation of 23 human papilloma virus genotypes. Jundishapur J. Microbiol. 2016; 9(4): e32728.
11. WHO/ICO Information Centre on HPV and Cervical Cancer. HPV and cervical cancer in the 2007 report. Vaccine. 2007; 25(Suppl. 3): C1-230.
12. Wang L., Wang P., Ren Y., Du J., Jiang J., Jia X. et al. Prevalence of High-Risk Human Papillomavirus (HR-HPV) genotypes and multiple infections in cervical abnormalities from Northern Xinjiang, China. PLoS One. 2016; 11(8): e160698.
Received 21.12.2016
Accepted 17.02.2017
About the Authors
Mzarelua Guranda Merabovna, the postgraduate student of Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: mzareluag@mail.ru
Nazarova Nico Mirzoevna, MD, Ph.D., Senior Researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: grab2@yandex.ru
Nekrasova Maria Evgenyevna, the postgraduate student of Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: mashenka_90@mail.ru
Sycheva Elena Gennadyevna, physicial, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381403. E-mail: el.bona@mail.ru
Burmenskaya Olga Vladimirovna, Senior Researcher, Researcher, Laboratory of Molecular Genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health and of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: o_bourmenskaya@oparina4.ru
Starodubtseva Natalia Leonidovna, Ph.D., Head of the Laboratory of Proteomics of Human Reproduction, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117497, Russia, Moscow, Ac. Oparina str.4. Tel.: +79164639867. E-mail: proteom.oparina4@gmail.com
Asaturova Alexandra Vjacheslavovna, Senior Researcher, 1st Pathology Department, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4
Khabas Gregory Nikolaevich, Ph.D., Head of the Department of combined and integrated methods of treatment of gynecological diseases, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: g_khabas@oparina4.ru
Pavlovich Stanislav Vladislavovich, Ph.D., docent, academic secretary of the Federal State Budget Institution, Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79161893718. E-mail: s_pavlovich@oparina4.ru
For citations: Mzarelua G.M., Nazarova N.M., Nekrasova M.E., Sycheva E.G., Bourmenskaya O.V., Starodubtseva N.L., Asaturova A.V., Habas G.N., Pavlovich S.V. Prevalence of HPV types in patients with cervical intraepithelial neoplasia of different severity. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (4): 94-100. (in Russian)
https://dx.doi.org/10.18565/aig.2018.4.94-100
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