Prediction of outcomes in assisted reproductive technology programs by determining telomere length and telomerase activity in cumulus cells and lymphocytes

Kraevaya E.E., Nalobin D.S., Glukhov A.I., Makarova N.P., Kalinina E.A.

1 Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow 117997, Ac. Oparina str. 4, Russia; 2 M.V. Lomonosov Moscow State University, Moscow 119991, Leninskie gori, 1, Russia
Objective. To carry out a systematic analysis of scientific data on the efficiency of determining telomere length and telomerase activity in follicular cumulus cells and peripheral blood lymphocytes, as well as a correlation of this indicator with the outcomes of in vitro fertilization programs.
Material and methods. The review includes the data of foreign and Russian articles published in the past 7 years and found in Pubmed on this topic.
Results. Based on the analyzed literature data, the authors have concluded that the study of telomere length and telomerase activity in the follicular cumulus cells and peripheral blood lymphocytes is a relevant and promising method for predicting the outcomes of assisted reproductive technology programs.
Conclusion. There is a need for further investigations assessing the correlation of telomere length and telomerase activity in the follicular cumulus cells and peripheral blood lymphocytes with the quality of oocytes and embryos, as well as with the effectiveness of infertility treatment programs involving in vitro fertilization techniques.

Keywords

assisted reproductive technologies
telomeres
telomerase activity
cumulus cells
oocyte
lymphocyte
in vitro fertilization

References

1. Эбзеева М.В., Калинина Е.А., Кузьмичев Л.Н. Современные подходы к стимуляции суперовуляции в программах ВРТ. Проблемы репродукции. 2009; 4: 47-9. [Ebzeeva M.V., Kalinina E.A., Kuzmichev L.N. Modern approaches to stimulation of superovulation in ART programs. Problemyi reproduktsii. 2009; 4: 47-9. (in Russian)]

2. Смольникова В.Ю., Калинина Е.А., Краснощока О.Е., Донников А.Е., Бурменская О.В., Трофимов Д.Ю., Сухих Г.Т. Возможности неинвазивной оценки состояния ооцита и эмбриона при проведении программ ВРТ по профилю экспрессии мРНК факторов роста в фолликулярной жидкости. Акушерство и гинекология. 2014; 9: 36-43. [Smolnikova V.Yu., Kalinina E.A., Krasnoshchoka O.E., Donnikov A.E., Burmenskaya O.E., Trofimov D.Yu., Sukhikh G.T. Possibilities for noninvasive oocyte and embryo evaluation when implementing assisted reproductive technology programs for follicular-fluid growth factor mRNA expression. Akusherstvo i ginekologiya/Obstetrics and gynecology. 2014; 9: 36-43. (in Russian)]

3. Milewski R., Czerniecki J., Kuczyńska A. Morphokinetic parameters as a source of information concerning embryo developmental and implantation potential. Ginekol. Pol. 2016; 87(10): 677-84. doi: 10.5603/GP.2016.0067.

4. Рубцова М.П., Василькова Д.П., Малявко А.Н., Нарайкина Ю.В., Зверева М.Э., Донцова О.А. Функции теломеразы: удлинение теломер и не только. Acta Naturae. 2012; 4(2): 6-23. [Rubtsova M.P., Vasilkova D.P., Malyavko A.N., Naraykina Yu.V., Zvereva M.E., Dontsova O.A. Telomerase functions: lengthening telomeres and not only. Acta Naturae. 2012; 4(2): 6-23. (in Russian)]

5. Егоров Е.Е. Теломеры, теломераза, канцерогенез и мера здоровья. Клиническая онкогематология. Фундаментальные исследования и клиническая практика. 2010; 3(2): 184-97. [Egorov E.E. Telomeres, telomerase, carcinogenesis and a measure of health. Klinicheskaya onkogematologiya. Fundamentalnyie issledovaniya i klinicheskaya praktika. 2010; 3(2): 184-97. (in Russian)]

6. Михельсон В.М., Гамалей И.А. Теломерный механизм старения. Saarbrucken: Palmarium Academic Publishing; 2013. 86с. [Mihelson V.M., Gamaley I.A. The telomeric mechanism of aging. Saarbrucken: Palmarium Academic Publishing; 2013. 86p. (in Russian)]

7. Kornicka K., Marycz K., Marędziak M., Tomaszewski K.A., Nicpoń J. The effects of the DNA methyltranfserases inhibitor 5-Azacitidine on ageing, oxidative stress and DNA methylation of adipose derived stem cells. J. Cell. Mol. Med. 2017; 21(2): 387-401. doi: 10.1111/jcmm.12972.

8. Позднякова А.А., Володина М.А., Рштуни С.Д., Марченко Л.А., Высоких М.Ю. Митохондриальная дисфункция как одна из возможных причин нарушения фолликуло- и стероидогенеза при преждевременной недостаточности яичников. Акушерство, гинекология и репродукция. 2015; 5: 55-65. [Pozdnyakova A.A., Volodina M.A., Rshtuni S.D., Marchenko L.A., Vyisokih M.Yu. Mitochondrial dysfunction as one of the possible causes of follicle and steroidogenesis disorders in premature ovarian failure. Akusherstvo, ginekologiya i reproduktsiya. 2015; 5: 55-65. (in Russian)]

9. Храмова Ю.В. Новые экспериментальные подходы к изучению фолликулогенеза in vitro и манипуляциям с преимплантационными эмбрионами млекопитающих: дисс. … канд. биол. наук. М.; 2015. [Khramova Yu.V. New experimental approaches to the study of folliculogenesis in vitro and manipulations with preimplantation embryos of mammals: diss. ... cand. biol. sciences. Moscow; 2015. (in Russian)]

10. Kim N.W., Piatyszek M.A., Prowse K.R., Harley C.B., West M.D., Ho P.L. et al. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994; 266(5193): 2011-5. doi: 10.1126.

11. Ahmed R., Roger L., Costa Del Amo P., Miners K.L., Jones R.E., Boelen L. Human stem cell-like memory T cells are maintained in a state of dynamic flux. Cell Rep. 2016; 17(11): 2811-8. doi: 10.1016/j.celrep.2016.11.037.

12. Conomos D., Pickett H.A., Reddel R.R. Alternative lengthening of telomeres: remodeling the telomere architecture. Front. Oncol. 2013; 3: 27. doi: 10.3389/fonc.2013.00027. eCollection 2013.

13. Azari M., Kafi M., Ebrahimi B., Fatehi R., Jamalzadeh M. Oocyte maturation, embryo development and gene expression following two different methods of bovine cumulus-oocyte complexes vitrification. Vet. Res. Commun. 2017; 41(1): 49-56. doi: 10.1007/s11259-016-9671-8.

14. Черток В.М., Зенкина В.Г., Каргалова Е.П. Функциональная морфология яичника. Монография. Владивосток: Медицина ДВ; 2015. [Chertok V.M., Zenkina V.G., Kargalova E.P. Functional morphology of the ovary. Monograph. Vladivostok: Medicine of the Far East; 2015. (in Russian)]

15. Palmerini M.G., Nottola S.A., Leoni G.G., Succu S., Borshi X., Berlinguer F. et al. In vitro maturation is slowed in prepubertal lamb oocytes: ultrastructural evidences. Reprod. Biol. Endocrinol. 2014; 12: 115. doi: 10.1186/1477-7827-12-115.

16. Chronowska E. High-throughput analysis of ovarian granulosa cell transcriptome. Biomed. Res. Int. 2014; 2014: 213570.

17. Kossowska-Tomaszczuk K., De Geyter C., De Geyter M., Martin I., Holzgreve W., Scherberich A., Zhang H. The multipotency of luteinizing granulosa cells collected from mature ovarian follicles. Stem Cells. 2009; 27(1): 210-9. doi: 10.1634/stemcells.2008-0233.

18. Wang W., Chen H., Li R., Ouyang N. Telomerase activity is more significant for predicting the outcome of IVF treatment than telomere length in granulosa cells. Reproduction. 2014; 147(5): 649-57. doi: 10.1530/REP-13-0223.

19. Russo V., Berardinelli P., Capacchietti G., Scapolo P.A. Localization of the telomerase catalytic subunit (TERT) in pig ovarian follicles. Vet. Res. Commun. 2003; 27(Suppl. 1): 623-6. doi: 10.1023/B:VERC.0000014232.27281.ee.

20. Butts S., Riethman H. Correlation of telomere length and telomerase activity with occult ovarian insufficiency. J. Clin. Endocrinol. Metab. 2009; 94(12): 4835-43. doi: 10.1210/jc.2008-2269.

21. Pelosi E., Simonsick E. Dynamics of the ovarian reserve and impact of genetic and epidemiological factors on age of menopause. Biol. Reprod. 2015; 92(5): 130. doi: 10.1095/biolreprod.114.127381.

22. Тимофеева Е.В., Высоцкий Ю.А., Бородина Г.Н., Лопатина С.В. Закономерности структурно-клеточного строения яичников в онтогенезе. Бюллетень Восточно-Сибирского научного центра Сибирского отделения Российской академии медицинских наук. 2016; 1: 56-8. [Timofeeva E.V., Vyisotskiy Yu.A., Borodina G.N., Lopatina S.V. Regularities of the structural and cellular structure of the ovaries in ontogenesis. Bulletin of the East Siberian Scientific Center of the Siberian Branch of the Russian Academy of Medical Sciences. 2016; 1: 56-8. (in Russian)]

23. Hoyos L.R., Thakur M. Fragile X premutation in women: recognizing the health challenges beyond primary ovarian insufficiency. J. Assist. Reprod. Genet. 2017; 34(3): 315-23.

24. Mender I., Shay J.W. Telomerase repeated amplification protocol (TRAP). Bio Protoc. 2015; 5(22). pii: e1657.

25. Chen H., Wang W., Mo Y., Ma Y., Ouyang N., Li R. et al. Women with high telomerase activity in luteinised granulosa cells have a higher pregnancy rate during in vitro fertilisation treatment. J. Assist. Reprod. Genet. 2011; 28(9): 797-807.

26. Niu Z.H., Yun F. The clinical study of relationship between serum E2/P ratio on the day of HCG injection and embryo implantation. J. Pract. Obstet. Gynecol. 2005; 21: 370-2.

27. Czamanski-Cohen J., Sarid O. Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment. J. Assist. Reprod. Genet. 2015; 32(11): 1697-703. doi: 10.1007/s10815-015-0581-4.

28. Keefe D.L. Telomeres, reproductive aging, and genomic instability during early development. Reprod. Sci. 2016; 23(12): 1612-5.

Received 16.01.2017

Accepted 17.02.2017

About the Authors

Kraevaya Elizaveta Evgenievna, junior researcher of department of assisted reproductive technology in treating sterility, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina 4. Tel.: +74954382501. E-mail: e_kraevaya@ oparina4.ru
Nalobin Denis Sergeevich, PhD, senior researcher of the Biological Faculty, M.V. Lomonosov Moscow State University.
119991, Russia, Moscow, Leninskie gori, 1. Tel.: +74959394452. E-mail: Nalobin@mail.bio.msu.ru
Glukhov Alexandr Ivanovich, Sc.D., Professor, Leading Researcher of the Biological Faculty, M.V. Lomonosov Moscow State University.
119991, Russia, Moscow, Leninskie gori, 1. Tel.: +74959394452. E-mail: glukhov.ai@1msmu.ru
Makarova Natalia Petrovna, PhD, Senior Researcher of department of assisted reproductive technology in treating sterility, Research Center of Obstetrics,
Gynecology, and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina 4. Tel.: +74954381341. E-mail: np_makarova@ oparina4.ru
Kalinina Elena Anatolievna, MD, Associate Professor, The chief of department of assisted reproductive technology in treating sterility, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina 4. Tel.: +74954381341. E-mail: e_kalinina@oparina4.ru

For citations: Kraevaya E.E., Nalobin D.S., Glukhov A.I., Makarova N.P., Kalinina E.A. Prediction of outcomes in assisted reproductive technology programs by determining telomere length and telomerase activity in cumulus cells and lymphocytes. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (12): 26-30. (in Russian)
https://dx.doi.org/10.18565/aig.2017.12.26-30

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