Metabolites of the normal vaginal microflora increase the activity of antibiotics
Objective. Investigation of the influence of metabolites from the lactobacilli isolated from healthy women and LCR35 probiotic strain on sensitivity to antibiotics.Sgibnev A.V., Kremleva E.A.
Subject and methods. We studied the effects of H2O2, lactate, and surfactants obtained from 24 vaginal Lactobacillus spp. and metabolites from LCR35 on sensitivity to antibiotics of 172 strains of opportunistic bacteria.
Results. H2O2 and surfactants but no lactic acid were more effective for increasing the sensitivity of bacteria to antibiotics. Strain LCR35 increased sensitivity to antibiotics of all test strains, to a greater extent – G. vaginalis, E. coli and Klebsiella spp.
Conclusion. The phenomenon of potentiation of activity of antibiotic by metabolites from vaginal lactobacilli and LCR35 probiotic strain was detected. For effective treatment of inflammatory diseases by antibiotics should take into account the status of the normal microflora, and if necessary, compensate deficit by probiotic strains able to produce “assistants antibiotics”.
Keywords
antibiotic resistance
hydrogen peroxide
lactic acid
lactobacillus
probiotics
surface-active agents
Supplementary Materials
- Figure 1. Effect of metabolites from lactobacilli on susceptibility S. aureus to antibiotics (A) ...
- Figure 1. Effect of metabolites from lactobacilli on susceptibility CNS to antibiotics (B) ...
- Figure 1. Effect of metabolites from lactobacilli on susceptibility Streptococcus spp. s to antibiotics (C) ...
- Figure 2. Effect of metabolites from lactobacilli on susceptibility E.coli to antibiotics (A) ...
- Figure 2. Effect of metabolites from lactobacilli on susceptibility Klebsiella spp to antibiotics (B) ...
- Figure 2. Effect of metabolites from lactobacilli on susceptibility G. vaginalis to antibiotics (C) ...
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Received 07.02.2017
Accepted 17.02.2017
About the Authors
Sgibnev Andrey, doctor of biology, associate professor, leading researcher, Laboratory for the study of the mechanisms of formation microbiocenoses of humans; Instituteof Cellular and Intracellular Symbiosis, Ural Branch of RAS. 460000, Russia, Orenburg, Pionerskaja str. 11. Tel.: +73532770512. E-mail: andrej-sgibnev@yandex.ru
Kremleva Elena, MD, leading researcher, Laboratory for the study of the mechanisms of formation microbiocenoses of humans; Institute of Cellular and Intracellular Symbiosis, Ural Branch of RAS; Associate Professor, Department of Obstetrics and Gynecology „Orenburg State Medical University,”
Ministry of Healthcare of the Russian Federation. 460000, Russia, Orenburg, Pionerskaja str. 11. Tel.: +73532770512, +79198621236. E-mail: kremlena1@mail.ru
For citations: Sgibnev A.V., Kremleva E.A. Metabolites of the normal vaginal microflora
increase the activity of antibiotics. Akusherstvo i Ginekologiya/
Obstetrics and Gynecology. 2017; (3): 108-14. (in Russian)
http://dx.doi.org/10.18565/aig.2017.3.108-14
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