A new method of multimodal optical coherence tomography for the diagnosis of vulvar lichen sclerosus
Objective. To investigate whether an intravital multimodal optical coherence tomography (OCT) technique can be used to diagnose vulvar lichen sclerosus.Potapov A.L., Konovalova E.A., Sirotkina M.A., Vagapova N.N., Safonov I.K., Timakova A.A., Radenska-Lopovok S.G., Kuznetsov S.S., Zagainova E.V., Kuznetsova I.A., Gladkova N.D.
Materials and methods. The investigation enrolled 6 gynecology unit patients who were divided into 2 groups according to histological criteria for lichen sclerosus: 1) patients without signs of vulvar mucosal damage (a control group) and 2) those who had the late stage of lichen sclerosus (a study group). Immediately before a biopsy, the mucosal condition at the points studied was evaluated by multimodal OCT angiography and OCT lymphangiography.
Results. Multimodal OCT demonstrated decreases in the thickness of the epithelium, in the contrast of the epithelial/connective tissue border, and in the density of blood and lymphatic vessels compared to the intact mucosa. These changes reflect the criteria for the histological classification of lichen sclerosus.
Conclusion. The OCT signs lichen sclerosus were demonstrated to correspond with its histological features. OCT was shown to be a promising in vivo tool for the diagnosis of sclerotic lichen.
Keywords
multimodal OCT
OCT angiography
OCT lymphangiography
vulva
lichen sclerosis
blood vessels
lymphatic vessels
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Received 09.01.2020
Accepted 08.10.2020
About the Authors
Arseniy L. Potapov, student, Privolzhsky Research Medical University. Tel.: +7(915)956-02-51. E-mail: Arseniy1109@gmail.com. ORCID: 0000-0003-4343-2500.603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1.
Elizaveta A. Konovalova, student, Privolzhsky Research Medical University. Tel.: +7(920)074-42-43. E-mail: dr.elizaveta.konovalova.7@gmail.com.
603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1.
Marina A. Sirotkina, Ph.D., Head of Laboratory of High Resolution Microscopy and Gene Technologies, Research Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University. Tel.: +7(904)060-68-73. E-mail: sirotkina_m@mail.ru.
603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1.
Naylya N. Vagapova, gynecologist of the Ist gynecological department, N.A. Semashko Nizhny Novgorod Regional Clinical Hospital.
Tel.: +7(915)945-50-45. E-mail: vagapova_nelly@mail.ru. 603126, Russia, Nizhny Novgorod, Rodionova str., 190.
Ivan K. Safonov, Clinical Resident, Privolzhsky Research Medical University. Tel.: +7(960)179-36-50. E-mail: iv.safonov2017@yandex.ru.
603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1.
Anna A. Timakova, student, I.M. Sechenov First Moscow State Medical University (Sechenov University). Tel.: +7(915)218-26-31. E-mail: atimakova96@gmail.com.
119991, Russia, Moscow, Trubetskaya st., 8/2.
Stefka G. Radenska-Lopovok, MD, Professor, A.I. Strukov Department of Pathological Anatomy, I.M. Sechenov First Moscow State Medical University (Sechenov University). Tel.: +7(916)653-09-20. E-mail: radenska@mail.ru. 119991, Russia, Moscow, Trubetskaya st., 8/2.
Sergey S. Kuznetsov, MD, Professor, Head of Pathological and Anatomical Department, N.A. Semashko Nizhny Novgorod Regional Clinical Hospital.
Tel.: +7(905)010-77-84. E-mail: zunek@mail.ru. 603126, Russia, Nizhny Novgorod, Rodionova str., 190.
Elena V. Zagaynova, MD, Professor of RAS, Director of Research Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University.
Tel.: +7(951)918-53-67. E-mail: ezagaynova@gmail.com. 603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1.
Irina A. Kuznetsova, MD, Professor, Head of Pathological and Anatomical Department, Privolzhsky Research Medical University. Tel.: +7(906)357-46-23.
E-mail: irakuz2007@mail.ru. 603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1.
Natal’ya D. Gladkova, MD, Professor, Head of Laboratory of Optical Coherent Tomography, Scientific Research Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University. Tel.: +7(951)910-66-57. E-mail: natalia.gladkova@gmail.com. 603950, Russia, Nizhny Novgorod, Minin and Pozharsky sq., 10/1
For citation: Potapov A.L., Konovalova E.A., Sirotkina M.A., Vagapova N.N., Safonov I.K., Timakova A.A., Radenska-Lopovok S.G., Kuznetsov S.S., Zagainova E.V., Kuznetsova I.A., Gladkova N.D. A new method of multimodal optical coherence tomography for the diagnosis of vulvar lichen sclerosus.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2020; 12: 169-176 (in Russian)
https://dx.doi.org/10.18565/aig.2020.12.169-176
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