Methods for improving the outcomes of assisted reproductive technology programs through innovative approaches to selecting male germ cells

1. Hunter P. The long-term health risks of ART: Epidemiological data and research on animals indicate that in vitro fertilization might create health problems later in life. EMBO Rep. 2017; 18(7): 1061-4. https://dx.doi.org./10.15252/embr.201744479.
2. Messerlian C., Gaskins A.J. Epidemiologic approaches for studying assisted reproductive technologies: design, methods, analysis and interpretation. Curr. Epidemiol. Rep. 2017; 4(2): 124-32. https://dx.doi.org./10.1007/s40471-017-0105-0.
3. Bernardino R.L., Carrageta D.F., Sousa M., Alves M.G., Oliveira P.F. pH and male fertility: Making sense on pH homeodynamics throughout the male reproductive tract. Cell Mol. Life Sci. 2019; 76(19): 3783-800. https://dx.doi.org./10.1007/s00018-019-03170-w.
4. Carrageta D.F., Bernardino R.L., Soveral G., Calamita G., Alves M.G., Oliveira P.F. Aquaporins and male (in)fertility: Expression and role throughout the male reproductive tract. Arch. Biochem. Biophys. 2020; 679: 108222.https://dx.doi.org./10.1016/j.abb.2019.108222.
5. Henkel R.R., Schill W.B. Sperm preparation for ART. Reprod. Biol. Endocrinol. 2003; 1: 108. https://dx.doi.org./10.1186/1477-7827-1-108.
6. Oshio S., Kaneko S., Iizuka R., Mohri H. Effects of gradient centrifugation on human sperm. Arch. Androl. 1987; 19(1): 85-93. https://dx.doi.org./10.3109/01485018708986804.
7. Malvezzi H., Sharma R., Agarwal A., Abuzenadah A.M., Abu-Elmagd M. Sperm quality after density gradient centrifugation with three commercially available media: A controlled trial. Reprod. Biol. Endocrinol. 2014; 12: 121.https://dx.doi.org./10.1186/1477-7827-12-121.
8. Brahem S., Letaief K., Ben Ali H., Saad A., Mehdi M. Efficacy of the density gradient centrifugation method in eliminating sperm with aneuploidy. Andrologia. 2013; 45(3): 158-62. https://dx.doi.org./10.1111/j.1439-0272.2012.01327.x.
9. Quinn M.M., Jalalian L., Ribeiro S., Ona K., Demirci U., Cedars M.I., Rosen M.P. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum. Reprod. 2018; 33(8): 1388-93. https://dx.doi.org./10.1093/humrep/dey239.
10. Takeshima T., Yumura Y., Kuroda S., Kawahara T., Uemura H., Iwasaki A. Effect of density gradient centrifugation on reactive oxygen species in human semen. Syst. Biol. Reprod. Med. 2017; 63(3): 192-8. https://dx.doi.org./10.1080/19396368.2017.1294214.
11. Beydola T., Sharma R.K., Lee W., Agarwal A. Sperm preparation and selection techniques. In: Rizk B., Aziz N., Agarwal A., Sabanegh E., eds. Male infertility practice. New Delhi: Jaypee Brothers Medical Publishers; 2013: 244-51.
12. Huang H., Huang P., Yao D. Enhanced efficiency of sorting sperm motility utilizing a microfluidic chip. Microsystem Technologies. 2015; 23(2): 305-12. https://dx.doi.org./10.1007/s00542-015-2495-6.
13. Simon L., Shamsi M.B., Carrell D.T. Sperm selection techniques and their relevance to ART. In: Schatten H., ed. Human reproduction : updates and new horizons. Wiley-Blackwell; 2016: 1-43.
14. Smith G.D., Takayama S. Application of microfluidic technologies to human assisted reproduction. Mol. Hum. Reprod. 2017; 23(4): 257-68.https://dx.doi.org./10.1093/molehr/gaw076.
15. Marzano G., Chiriacò M.S., Primiceri E., Dell'Aquila M.E., Ramalho-Santos J., Zara V. et al. Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities. Biotechnol. Adv. 2020; 40: 107498. https://dx.doi.org./10.1016/j.biotechadv.2019.107498.
16. Oseguera-Lopez I., Ruiz-Diaz S., Ramos-Ibeas P., Perez-Cerezales S. Novel techniques of sperm selection for improving IVF and ICSI outcomes. Front. Cell Dev. Biol. 2019; 7: 298. https://dx.doi.org./10.3389/fcell.2019.00298.
17. Samuel R., Feng H., Jafek A., Despain D., Jenkins T., Gale B. Microfluidic-based sperm sorting & analysis for treatment of male infertility. Transl. Androl. Urol. 2018; 7(Suppl. 3): S336-47. https://dx.doi.org./10.21037/tau.2018.05.08.
18. Cho B.S., Schuster T.G., Zhu X., Chang D., Smith G.D., Takayama S. Passively driven integrated microfluidic system for separation of motile sperm. Anal. Chem. 2003; 75(7): 1671-5. https://dx.doi.org./10.1021/ac020579e.
19. Schuster T.G., Cho B., Keller L.M., Takayama S., Smith G.D. Isolation of motile spermatozoa from semen samples using microfluidics. Reprod. Biomed. Online. 2003; 7(1): 75-81. https://dx.doi.org./10.1016/s1472-6483(10)61732-4.
20. Wu J.K., Chen P.C., Lin Y.N., Wang C.W., Pan L.C., Tseng F.G. High-throughput flowing upstream sperm sorting in a retarding flow field for human semen analysis. Analyst. 2017; 142(6): 938-44. https://dx.doi.org./10.1039/c6an02420c.
21. Wu S., Zhang Z., Zhou X., Liu H., Xue C., Zhao G. et al. Nanomechanical sensors for direct and rapid characterization of sperm motility based on nanoscale vibrations. Nanoscale. 2017; 9(46): 18258-67. https://dx.doi.org./10.1039/c7nr03688d.
22. Chen C.Y., Chiang T.C., Lin C.M., Lin S.S., Jong D.S., Tsai V.F.S. et al. Sperm quality assessment via separation and sedimentation in a microfluidic device. Analyst. 2013; 138(17): 4967-74. https://dx.doi.org./10.1039/c3an00900a.
23. You J.B., Wang Y., McCallum C., Tarlan F., Hannam T., Lagunov A. et al. Live sperm trap microarray for high throughput imaging and analysis. Lab. Chip. 2019; 19(5): 815-24. https://dx.doi.org./10.1039/c8lc01204k.
24. Kumar M., Ardekani A.M. The effect of external shear flow on the sperm motility. Soft Matter. 2019; 15(31): 6269-6277. https://dx.doi.org./10.1039/c9sm00717b.
25. Narayanamurthy V., Jeroish Z.E., Bhuvaneshwari K.S., Bayat P., Premkumar R., Samsuri F., Yusoff M.M. Advances in passively driven microfluidics and lab-on-chip devices: a comprehensive literature review and patent analysis. RSC Adv. 2020; 10(20): 11652-80. https://dx.doi.org./10.1039/D0RA00263A.
26. Zhang X., Khimji I., Gurkan U.A., Safaee H., Catalano P.N., Keles H.O. et al. Lensless imaging for simultaneous microfluidic sperm monitoring and sorting. Lab. Chip. 2011; 11(15): 2535-40. https://dx.doi.org./10.1039/c1lc20236g.
27. Yan Y., Zhang B., Fu Q., Wu J., Liu R. A fully integrated biomimetic microfluidic device for evaluation of sperm response to thermotaxis and chemotaxis. Lab. Chip. 2021; 21(2): 310-18. https://dx.doi.org./10.1039/d0lc00845a.
28. Simchi M., Riordon J., You J.B., Wang Y., Xiao S., Lagunov A. et al. Selection of high-quality sperm with thousands of parallel channels. Lab Chip. 2021; 21(12): 2464-75. https://dx.doi.org./10.1039/d0lc01182g.
29. Rappa K.L., Rodriguez H.F., Hakkarainen G.C., Anchan R.M., Mutter G.L., Asghar W. Sperm processing for advanced reproductive technologies: Where are we today? Biotechnol. Adv. 2016; 34(5): 578-87. https://dx.doi.org./10.1016/j.biotechadv.2016.01.007.
30. Vizziello G., Baldini A., Porcelli G., Gliozheni E., Kati K., Baldini D., Vizziello D. A new simple and quick method for sperm preparation and selection prior to ICSI procedure. Curr. Trends Clin. Embriol. 2015; 2: 101-2. https://dx.doi.org/10.11138/cce/2015.2.3.101.
31. Baldini D., Baldini A., Silvestris E., Vizziello G., Ferri D., Vizziello D. A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT). Reprod. Biol. Endocrinol. 2020; 18(1): 88.https://dx.doi.org/10.1186/s12958-020-00642-8.
32. Debnath D., Ghosh P.K., Misko V.R., Li Y., Marchesoni F., Nori F. Enhanced motility in a binary mixture of active nano/microswimmers. Nanoscale. 2020; 12(17): 9717-26. https://dx.doi.org/10.1039/d0nr01765e.
33. Kishi K., Ogata H., Ogata S., Mizusawa Y., Okamoto E., Matsumoto Y. et al. Frequency of sperm DNA fragmentation according to selection method: Comparison and relevance of a microfluidic device and a swim-up procedure. J. Clin. Diagn. Res. 2015; 9(11): QC14-6. https://dx.doi.org/10.7860/JCDR/2015/10332.6811.
34. Nosrati R., Vollmer M., Eamer L., San Gabriel M.C., Zeidan K., Zini A., Sinton D. Rapid selection of sperm with high DNA integrity. Lab. Chip. 2014; 14(6): 1142-50. https://dx.doi.org/10.1039/c3lc51254a.
35. Quinn M.M., Jalalian L., Ribeiro S., Ona K., Demirci U., Cedars M.I., Rosen M.P. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum. Reprod. 2018; 33(8): 1388-93. https://dx.doi.org/10.1093/humrep/dey239.
36. Shirota K., Yotsumoto F., Itoh H., Obama H., Hidaka N., Nakajima K., Miyamoto S. Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage. Fertil. Steril. 2016; 105(2): 315-21.e1. https://dx.doi.org/10.1016/j.fertnstert.2015.10.023.
37. Yildiz K., Yuksel S. Use of microfluidic sperm extraction chips as an alternative method in patients with recurrent in vitro fertilisation failure. J. Assist. Reprod. Genet. 2019; 36(7): 1423-9. https://dx.doi.org/10.1007/s10815-019-01480-3.
38. Yetkinel S., Kilicdag E.B., Aytac P.C., Haydardedeoglu B., Simsek E., Cok T. Effects of the microfluidic chip technique in sperm selection for intracytoplasmic sperm injection for unexplained infertility: A prospective, randomized controlled trial. J. Assist. Reprod. Genet. 2019; 36(3): 403-9. https://dx.doi.org/10.1007/s10815-018-1375-2.
39. Feng H., Jafek A., Samuel R., Hotaling J., Jenkins T.G., Aston K.I., Gale B.K. High efficiency rare sperm separation from biopsy samples in an inertial focusing device. Analyst. 2021; 146(10): 3368-77. https://dx.doi.org/10.1039/d1an00480h.
40. Ishijima S.A., Okuno M., Mohri H. Zeta potential of human X- and Y-bearing sperm. Int. J. Androl. 1991; 14(5): 340-7. https://dx.doi.org/10.1111/j.1365-2605.1991.tb01102.x.
41. Kirchhoff C., Hale G. Cell-to-cell transfer of glycosylphosphatidylinositol-anchored membrane proteins during sperm maturation. Mol. Hum. Reprod. 1996; 2(3): 177-84. https://dx.doi.org/10.1093/molehr/2.3.177.
42. Chan P.J., Jacobson J.D., Corselli J.U., Patton W.C. A simple zeta method for sperm selection based on membrane charge. Fertil. Steril. 2006; 85(2): 481-6. https://dx.doi.org/10.1016/j.fertnstert.2005.07.1302.
43. Della Giovampaola C., Flori F., Sabatini L., Incerti L., La Sala G.B., Rosati F., Focarelli R. Surface of human sperm bears three differently charged CD52 forms, two of which remain stably bound to sperm after capacitation. Mol. Reprod. Dev. 2001; 60(1): 89-96. https://dx.doi.org/10.1002/mrd.1065.
44. Khajavi N.A., Razavi S., Mardani M., Tavalaee M., Deemeh M.R., Nasr-Esfahani M.H. Can Zeta sperm selection method, recover sperm with higher DNA integrity compare to density gradient centrifugation? Iran. J. Reprod. Med. 2009; 7(2): 73-7.
45. Kheirollahi-Kouhestani M., Razavi S., Tavalaee M., Deemeh M.R., Mardani M., Moshtaghian J., Nasr-Esfahani M.H. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum. Reprod. 2009; 24(10): 2409-16. https://dx.doi.org/10.1093/humrep/dep088.
46. Zarei-Kheirabadi M., Shayegan Nia, E., Tavalaee M., Deemeh M.R., Arabi M., Forouzanfar M. et al. Evaluation of ubiquitin and annexin V in sperm population selected based on density gradient centrifugation and zeta potential (DGC-Zeta). J. Assist. Reprod. Genet. 2012; 29(4): 365-71. https://dx.doi.org/10.1007/s10815-011-9689-3.
47. Nasr Esfahani M.H., Deemeh M.R., Tavalaee M., Sekhavati M.H., Gourabi H. Zeta sperm selection improves pregnancy rate and alters sex ratio in male factor infertility patients: A double-blind, randomized clinical trial. Int. J. Fertil. Steril. 2016; 10(2): 253-60. https://dx.doi.org/10.22074/ijfs.2016.4917.
48. Henkel R. Sperm preparation: State-of-the-art–physiological aspects and application of advanced sperm preparation methods. Asian J. Androl. 2012; 14(2): 260-9. https://dx.doi.org/10.1038/aja.2011.133.
49. Glander H.J., Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: A rapid assay for detection of membrane changes after cryostorage. Mol. Hum. Reprod. 1999; 5(2): 109-15. https://dx.doi.org/10.1093/molehr/5.2.109.
50. Vermes I., Haanen C., Steffens-Nakken H., Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods. 1995; 184(1): 39-51. https://dx.doi.org/10.1016/0022-1759(95)00072-i.
51. Grunewald S., Paasch U., Glander H.J. Enrichment of non-apoptotic human spermatozoa after cryopreservation by immunomagnetic cell sorting. Cell Tissue Bank. 2001; 2(3): 127-33. https://dx.doi.org/10.1023/A:1020188913551.
52. Said T.M., Land J.A. Effects of advanced selection methods on sperm quality and ART outcome: A systematic review. Hum. Reprod. Update. 2011; 17(6): 719-33. https://dx.doi.org/10.1093/humupd/dmr032.
53. Grunewald S., Miska W., Miska G., Rasch M., Reinhardt M., Glander H.J., Paasch U. Molecular glass wool filtration as a new tool for sperm preparation. Hum. Reprod. 2007; 22(5): 1405-12. https://dx.doi.org/10.1093/humrep/dem015.
54. Muratori M., Maggi M., Spinelli S., Filimberti E., Forti G., Baldi E. Spontaneous DNA fragmentation in swim-up selected human spermatozoa during long term incubation. J. Androl. 2003; 24(2): 253-62. https://dx.doi.org/10.1002/j.1939-4640.2003.tb02670.x.
55. Berteli T.S., Da Broi M.G., Martins W.P., Ferriani R.A., Navarro P.A. Magnetic-activated cell sorting before density gradient centrifugation improves recovery of high-quality spermatozoa. Andrology. 2017; 5(4): 776-82. https://dx.doi.org/10.1111/andr.12372.
56. Lee T.H., Liu C.H., Shih Y.T., Tsao H.M., Huang C.C., Chen H.H., Lee M.S. Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility. Hum. Reprod. 2010; 25(4): 839-46. https://dx.doi.org/10.1093/humrep/deq009.
57. Rawe V.Y., Alvarez C.R., Uriondo H.W., Papier S., Miasnik S., Nodar F. ICSI outcome using Annexin V columns to select non-apoptotic spermatozoa. Fertil. Steril. 2009; 92(3, Suppl.): S73-4. https://dx.doi.org/10.1016/j.fertnstert.2009.07.284.
58. Said T., Agarwal A., Grunewald S., Rasch M., Baumann T., Kriegel C., Paasch U. Selection of nonapoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: An in vitro model. Biol. Reprod. 2006; 74(3): 530-7. https://dx.doi.org/10.1095/biolreprod.105.046607.
59. Zhang H., Xuan X., Yang S., Li X., Xu C., Gao X. Selection of viable human spermatozoa with low levels of DNA fragmentation from an immotile population using density gradient centrifugation and magnetic-activated cell sorting. Andrologia. 2018; 50(1): e12821. https://dx.doi.org/10.1111/and.12821.
60. Romany L., Garrido N., Motato Y., Aparicio B., Remohi J., Meseguer M. Removal of annexin V-positive sperm cells for intracytoplasmic sperm injection in ovum donation cycles does not improve reproductive outcome: A controlled and randomized trial in unselected males. Fertil. Steril. 2014; 102(6): 1567-75.e1. https://dx.doi.org/10.1016/j.fertnstert.2014.09.001.
61. Said T.M., Grunewald S., Paasch U., Rasch M., Agarwal A., Glander H.J. Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates. Fertil. Steril. 2005; 83(5): 1442-6. https://dx.doi.org/10.1016/j.fertnstert.2004.11.052.
62. Gil M., Sar-Shalom V., Melendez Sivira Y., Carreras R., Checa M.A. Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: A systematic review and meta-analysis. J. Assist. Reprod. Genet. 2013; 30(4): 479-85. https://dx.doi.org/10.1007/s10815-013-9962-8.
63. Dirican E.K., Özgün O.D., Akarsu S., Akın K.O., Ercan Ö., Uğurlu M. et al.Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction. J. Assist. Reprod. Genet. 2008; 25(8): 375-81. https://dx.doi.org/10.1007/s10815-008-9250-1.
64. Ziarati N., Tavalaee M., Bahadorani M., Nasr Esfahani M.H. Clinical outcomes of magnetic activated sperm sorting in infertile men candidate for ICSI. Hum Fertil (Camb). 2019; 22(2): 118-25. https://dx.doi.org/10.1080/14647273.2018.1424354.
65. Zhuo L., Kimata K. Cumulus oophorus extracellular matrix: its construction and regulation. Cell Struct. Funct. 2001; 26(4): 189-96. https://dx.doi.org/10.1247/csf.26.189.
66. Hasegawa J., Yanaihara A., Iwasaki S., Otsuka Y., Negishi M., Akahane T., Okai T. Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality. Hum. Reprod. 2005; 20(8): 2194-200. https://dx.doi.org/10.1093/humrep/dei005.
67. Franken D.R., Bastiaan H.S. Can a cumulus cell complex be used to select spermatozoa for assisted reproduction? Andrologia. 2009; 41(6): 369-76.https://dx.doi.org/10.1111/j.1439-0272.2009.00938.x.
68. Naknam W., Salang L., Sothornwit J., Amnatbuddee S., Seejorn K., Pongsritasana T., Sukkasame S. Effect of sperm selection method by cumulus oophorus complexes and conventional sperm preparation method on sperm quality and DNA fragmentation for assisted reproduction techonology. Eur. J. Obstet. Gynecol. Reprod. Biol. 2019; 243: 46-50. https://dx.doi.org/10.1016/j.ejogrb.2019.10.004.
69. Yazdanpanah Z., Nasrabadi M.H., Piravar Z. Comparison of three sperm selection methods for ICSI-DGC, Cumulus column, and incubation with supernatant product of adipose tissue-derived adult stem cells: An experimental study. Int. J. Reprod. Biomed. 2021; 19(1): 97-104. https://dx.doi.org/10.18502/ijrm.v19i1.8184.
70. Wang C., Feng G., Shu J., Zhou H., Zhang B., Chen H. et al. Cumulus oophorus complexes favor physiologic selection of spermatozoa for intracytoplasmic sperm injection. Fertil. Steril. 2018; 109(5): 823-31. https://dx.doi.org/10.1016/j.fertnstert.2017.12.026.
71. Fazaeli H., Faeze D., Naser K., Maryam S., Mohammad M., Mahdieh G., Reza T.Q. Introducing of a new experimental method in semen preparation: supernatant product of adipose tissue: derived mesenchymal stem cells (SPAS). JFIV Reprod. Med. Genet. Stem Cell Biol. 2016; 4(2): 178.https://dx.doi.org/10.4172/2375-4508.1000178.
72. Li C.Y., Wu X.Y., Tong J.B., Yang X.X., Zhao J.I., Zheng Q.F. et al. Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. Stem Cell Res Ther. 2015; 6(1): 55. https://dx.doi.org/10.1186/s13287-015-0066-5.
73. Fazaeli H., Davoodi F., Kalhor N., Qomi R.T. The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT. Int. J. Reprod. Biomed. 2018; 16(3):199-208.
74. Kubasek W.L., Wang Y., Thomas G.A., Patapoff T.W., Schoenwaelder K.H., Van der Sande J.H., Peticolas W.L. Raman spectra of the model B-DNA oligomer d(CGCGAATTCGCG)2 and of the DNA in living salmon sperm show that both have very similar B-type conformations. Biochemistry. 1986; 25(23): 7440-5. https://dx.doi.org/10.1021/bi00371a028.
75. Huser T., Orme C.A., Hollars C.W., Corzett M.H., Balhorn R. Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. J. Biophotonics. 2009; 2(5): 322-32. https://dx.doi.org/10.1002/jbio.200910012.
76. Meister K., Schmidt D.A., Bründermann E., Havenith M. Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa. Analyst. 2010; 135(6): 1370-4. https://dx.doi.org/10.1039/b927012d.
77. Sánchez V., Redmann K., Wistuba J., Wübbeling F., Burger M., Oldenhof H. et al. Oxidative DNA damage in human sperm can be detected by Raman microspectroscopy. Fertil. Steril. 2012; 98(5): 1124-9.e1-3. https://dx.doi.org/10.1016/j.fertnstert.2012.07.1059.
78. Jahmani M.Y., Hammadeh M.E., Al Smadi M.A., Baller M.K. Label-free evaluation of chromatin condensation in human normal morphology sperm using Raman spectroscopy. Reprod. Sci. 2021; 28(9): 2527-39.https://dx.doi.org/10.1007/s43032-021-00494-6.
79. Pachetti M., D'Amico F., Zupin L., Luppi S., Martinelli M., Crovella S. et al. Strategies and perspectives for UV resonance Raman applicability in clinical analyses of human sperm RNA. Int. J. Mol. Sci. 2021; 22(23): 13134.https://dx.doi.org/10.3390/ijms222313134.
80. Дударова А.Х., Смольникова В.Ю., Зобова А.В., Макарова Н.П., Калинина Е.А., Андреева М.Г., Наими З.М.С. Достижения и перспективы в преодолении мужского бесплодия в программах вспомогательных репродуктивных технологий с использованием различных методик селекции сперматозоидов. Акушерство и гинекология. 2016; 2: 28-34.[Dudarova A.Kh., Smolnikova V.Yu., Zobova A.V., Makarova N.P., Kalinina E.A., Andreeva M.G., Naimi Z.M.S. Advances and prospects for overcoming male infertility in assisted reproductive technology programs using different sperm selection procedures. Obstetrics and Gynecology. 2016; 2: 28-34. (in Russian)]. https://dx.doi.org/10.18565/aig.2016.2.28-34.

Received 11.04.2022

Accepted 01.06.2022