A liquid-based cytology technique for the diagnosis of precancerous fallopian tube lesions in ovarian tumors
Objective. To examine cytological smears of a fimbrial section of the fallopian tube to identify the cytological characteristics of ovarian serous tumors and the possibilities of using the technique for the diagnosis of ovarian serous cancer in its early stages.Asaturova A.V., Sannikova M.V., Khabas G.N., Timofeeva A.V.
Subjects and methods. The investigation enrolled 21 patients (mean age 45.6±13.7 years), in whom 36 smears were taken from 36 fallopian tubes. The material was placed in a container having a patented concentrated solution that was a buffered medium with low methanol content and microscopically evaluated after using Pappanicolaou staining. When atypical glandular cells were detected, an immunocytochemical study was conducted using antibodies to p16INK4a (clone E6H4, RTU) and Ki67 (clone 274-11AC3, RTU) as a set of CINtec PLUS (Roche) and antibodies to p53 (clone DO-7, RTU, Dako).
Results. Histological diagnosis revealed papillary fallopian tube hyperplasia in the presence of serous borderline tumors in all cases; however, there were no clear cytological signs of this disease. The cytological changes in high-grade ovarian carcinomas, which were largely concerned with the features of the cell nuclear apparatus, were most characteristic and immunocytochemically confirmed using the markers P16, p53, and Ki-67.
Conclusion. Further study of the cytological features of smears in intraepithelial pathology of the fallopian tube is necessary for the elaboration of cytological criteria that will be able to diagnose early-stage intraepithelial lesions and to create a platform for the development of new approaches to searching for the early diagnostic markers of ovarian serous carcinomas.
Keywords
liquid-based cytology
ovarian serous carcinomas
papillary fallopian tube hyperplasia
serous tubal intraepithelial carcinoma
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Received 07.09.2016
Accepted 11.11.2016
About the Authors
Asaturova Alexandra Vyacheslavovna, Ph.D., senior researcher, Department of Pathological Anatomy, Research Center of Obstetrics, Gynecology, and Perinatology,Ministry of Health of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382311. E-mail: a_asaturova@oparina4.ru
Sannikova Maya Viktorovna, Ph.D., Research Associate, Department of Combined and Comprehensive Methods of Gynecologic Diseases Treatment,
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: m_sannikova@oparina4.ru, mvs6103@gmail.com
Khabas Grigory Nikolaevich, Ph.D., head of the department of combined and complex methods of treatment of gynecological diseases, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: g_khabas@oparina4.ru
Timofeeva Anzhelika Vladimirovna, Ph.D., Senior Researcher, Laboratory of Molecular Pathophysiology, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117485, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: v_timofeeva @ oparina4.ru.
For citations: Asaturova A.V., Sannikova M.V., Khabas G.N., Timofeeva A.V. A liquid-based cytology technique for the diagnosis of precancerous fallopian tube lesions in ovarian tumors. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (5): 104-12. (in Russian)
http://dx.doi.org/10.18565/aig.2017.5.104-12
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