Study of embryo metabolic products in culture media as a tool for determining the implantation potential

Zorina I.M., Smolnikova V.Yu., Bobrov M.Yu.

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow 117997, Ac. Oparina str. 4, Russia
The review gives an update on the analysis of embryo metabolic products in nutrient media and on the possible use of a number of metabolites as markers for successful embryo implantation.
Material and methods. The review includes the data of foreign and Russian articles published in the past 10 years and found in Pubmed on this topic.
Results. The media for the cultivation of embryos at various stages of development are a unique study object, as they carry information about the energetic and metabolic activities and the signaling systems of the embryo. The study of the aspects of human embryo life is a fundamental task and it is of undoubted interest because it will be able to expand our understanding of the processes of early development, to develop new technologies improving the outcomes of assisted reproductive technology (ART) programs, and to introduce them into clinical practice.
Conclusion. Further studies and assessment of the molecular components of nutrient media are a promising area in searching for the markers of successful embryo implantation with the subsequent development of a clinical pregnancy and the birth of a healthy baby to enhance the efficiency of treatment using ART techniques.
Scientifically substantiated evidence on the molecular composition of cultivation media will be able to develop an additional noninvasive procedure to choose an embryo for selective transfer, which will make it possible to elaborate an individual management algorithm for women in ART programs.

Keywords

assisted reproductive technologies
in vitro fertilization
cultivation medium
embryo cultivation
embryo implantation
mass-spectrometry
microRNA
spectrometry

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Received 03.11.2016

Accepted 11.11.2016

About the Authors

Zorina I.M., Post-graduate student, Department of assistive technologies in infertility treatment, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79267126561. E-mail: afinna_153@mail.ru
Smolnikova V.U., PhD, Leading researcher, Department of assistive technologies in infertility treatment, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: veronika.smolnikova@mail.ru
Bobrov M.Yu., MD, Head of laboratory of molecular pathophysiology, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. E-mail: mbobr@mail.ru

For citations: Zorina I.M., Smolnikova V.Yu., Bobrov M.Yu. Study of embryo metabolic products in culture media as a tool for determining the implantation potential.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (2): 11-6. (in Russian)
http://dx.doi.org/10.18565/aig.2017.2.11-6

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