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The Journal "Obstetrics and Gynecology"Journal HistoryAims and ScopePolicies on Conflict of Interest, Human and Animal Rights, and Informed ConsentEditorial ProcessData sharing statementAdvertising PolicyThe Process for Handling Cases Requiring Corrections, Retractions, and Editorial Expressions of ConcernEditorial BoardEditorial CounsilInformation for ProfessionalsNewsContacts
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Obstetrics and Gynegology2020№11
Cytoplasmic fragmentation of human preimplantation...

Cytoplasmic fragmentation of human preimplantation embryos

DOI
https://dx.doi.org/10.18565/aig.2020.11.61-70

Kirienko K.V., Apryshko V.P., Yakovenko S.A.

1) AltraVita Clinic of Human Reproduction, “IVF CENTER” LLC, Moscow, Russia; 2) Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, Russia
This investigation has attempted to generalize and systematize knowledge about the cytoplasmic embryo fragmentation phenomenon, to reveal the causes and mechanisms of its occurrence, and to describe its impact on embryo viability and clinical outcomes of IVF programs. Since human embryo fragmentation is most common during the embryological stage of IVF programs, the phenomenon itself has not been studied sufficiently. Rare attempts to investigate this phenomenon have narrowly focused on the association of the fragmentation with apoptosis. The reasons for this limited knowledge were ethical obstacles and practical difficulties associated with the use of human eggs and embryos for experiments. The lack of a suitable model among animal embryos and the relatively low occurrence and significance of fragmentation in experimental embryology (in mouse embryos) also contributed to the exclusion of the fragmentation phenomenon as a worthy object of investigation. The review included the data obtained over a fairly extensive period and an attempt was undertaken to comprehensively cover the topic of embryo fragmentation. Conclusion. Despite the fact that the effect of a low fragmentation fraction (<10%) on the effectiveness of ART programs is insignificant, a significant fragmentation fraction (> 25-30%) reduces embryo viability. The results of studies investigating the microsurgical removal of cytoplasmic fragments and its positive impact on the viability of embryos and the clinical outcomes of IVF programs are controversial and require further confirmation.

Keywords

embryo fragmentation
in vitro fertilization
developmental anomaly
embryo viability
Full text (in Russian)

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Received 30.06.2020

Accepted 23.10.2020

About the Authors

Konstantin V. Kirienko, PhD, leading embryologist of AltraVita IVF clinic. Tel.: +7(967)167-79-23. E-mail: kkiriyenko@rambler.ru. ORCID: 0000-0001-8713-6231.
4A, Nagornaya str., 117186, Moscow, Russia.
Valentina P. Apryshko, PhD, Head of Embryology department of AltraVita IVF clinic. Tel.: +7(910)409-28-13. E-mail: supermycolog@mail.ru.
4A, Nagornaya str., 117186, Moscow, Russia.
Sergey A. Yakovenko, PhD, G.M. of AltraVita IVF clinic; researcher of Lomonosov Moscow state University, Faculty of Physics, Biophisics Department.
Tel.: +7(903)790-90-18. E-mail: altravita@mail.ru. 4A, Nagornaya str., 117186, Moscow, Russia; 1/2 Leninskie gori, 119991, Moscow, Russia.

For citation: Kirienko K.V., Apryshko V.P., Yakovenko S.A. Cytoplasmic fragmentation of human preimplantation embryos.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2020; 11: 61-70 (in Russian).
https://dx.doi.org/10.18565/aig.2020.11.61-70

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