Autologous embryo-cumulus cell co-culturing in ART programs
The investigators have carried out a systems analysis of the data available in the modern literature on the role of cumulus cells, autologous embryo-cumulus cell co-culturing in the treatment of infertility with assisted reproductive technologies (ART). The paper describes the role of cumulus cells in the embryo implantation process using autologous co-culturing and cumulus-aid embryo transfer, which allows the ART programs to be individualized. The results of investigations confirm the relevance of the use of autologous embryo-cumulus cell co-culturing in the ART programs. Cumulus cells can become a reliable model for understanding the components of oocyte quality, for stimulating the ovaries, for assessing embryo development, and the rates of clinical pregnancy and healthy births in the ART programs.Asfarova G.R., Smolnikova V.Yu., Makarova N.P., Bobrov M.Yu., Kalinina E.A., Romanov E.A.
Keywords
infertility
assisted reproductive technologies
cumulus cells
embryo implantation
embryo culturing
co-culturing
microRNA
References
1. Mascarenhas M.N., Cheung H., Mathers C.D., Stevens G.A. Measuring infertility in populations: constructing a standard definition for use with demographic and reproductive health surveys. Popul. Health Metr. 2012; 10(1): 17.
2. Gunby J., Daya S. Assisted reproductive technologies (ART) in Canada: 2002 results from the Canadian ART Register. Fertil. Steril. 2006; 86(5): 1356–64.
3. Bissonnette F., Cohen J., Collins J., Cowan L., Dale S., Dill S. et al. Incidence and complications of multiple gestation in Canada: proceedings of an expert meeting. Reprod. Biomed. Online. 2007; 14(6): 773–90.
4. Исхаков И.Р., Исхакова Р.С. Культивирование эмбрионов человека на ранних этапах и свободнорадикальное окисление. Биорадикалы и антиоксиданты. 2015; 1: 40–4.
5. Громенко Ю.Ю., Исхаков И.Р. Влияние факторов оценки качества перенесенных эмбрионов на прогнозирование частоты наступления беременности в программах экстракорпорального оплодотворения. Медицинский вестник Башкортостана. 2012; 7(2): 27–30.
6. Савельева Г.М., Касьянова Г.В., Дронова М.А., Карачунская Е.М. Вспомогательные репродуктивные технологии: перинатальные исходы и состояние детей. Проблемы репродукции. 2014; 20(6): 35–9.
7. Егорова А.Т., Руппель Н.И., Маисеенко Д.А., Базина М.И. Течение беременности и родов после ВРТ. Проблемы репродукции. 2015; 21(4): 60–4.
8. Смольникова В.Ю., Калинина Е.А., Краснощока О.Е, Донников А.Е., Бурменская О.В., Трофимов Д.Ю., Сухих Г.Т. Возможности неинвазивной оценки состояния ооцита и эмбриона при проведении программ ВРТ по профилю экспрессии мРНК факторов роста в фолликулярной жидкости. Акушерство и гинекология. 2014; 9: 36–43.
9. Haouzi D., Assou S., Mahmoud K., Tondeur S., Rème T., Hedon B. et al. Gene expression profile of human endometrial receptivity: comparison between natural and stimulated cycles for the sam patients. Hum. Reprod. 2009; 24(6): 1436–45.
10. Кузьмичев Л.Н., Смольникова В.Ю., Калинина Е.А., Дюжева Е.В. Принципы комплексной оценки и подготовки эндометрия у пациенток программ вспомогательных репродуктивных технологий. Акушерство и гинекология. 2010; 5: 32–6.
11. Вартанян Э.В., Цатурова К.А., Маркин А.В. Преодоление неудач вспомогательных репродуктивных технологий при лечении бесплодных супружеских пар. Доктор. Ру. 2011; 9–2: 30–3.
12. Митюрина Е.В., Перминова С.Г., Амян Т.С. Причины повторных неудач имплантации в программе экстракорпорального оплодотворения. Акушерство и гинекология. 2016; 11: 34–40.
13. Широкова Д.В., Широкова Д.В., Калинина Е.А., Полина М.Л., Петров Ю.А. Морфофункциональная вариабельность эндометрия как основа дифференцированного лечения бесплодия. Современные проблемы науки и образования. 2015; 6: 270.
14. Белокурова М.В., Панина О.Б., Савельева Г.М., Самоходская Л.М., Садекова О.Н., Ткачук В.А., Крамаренко М.П. Аллельный полиморфизм генов ангиогенных факторов у пациенток с неудачными попытками экстракорпорального оплодотворения. Вестник Российского университета дружбы народов. Серия: Медицина. 2012; 5: 40–7.
15. Машкина Е.В., Коваленко К.А., Фомина Н.В., Покудина И.О. Полиморфизм генов цитокинов в тканях плаценты при невынашивании беременности. Фундаментальные исследования. 2013; 1–3: 580–4.
16. Boomsma C.M., Kavelaars A., Eijkemans M.J., Lentjes E.G., Fauser B.C., Heijnen C.J., Macklon N.S. Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF. Hum. Reprod. 2009; 24(6): 1427–35.
17. Кравчук Я.Н., Калугина А.С. Оценка рецептивности эндометрия с помощью биомаркеров. Журнал акушерства и женских болезней. 2012; 61(6): 61–5.
18. Крылова Ю.С., Кветной И.М., Айламазян Э.К. Рецептивность эндометрия: молекулярные механизмы регуляции имплантации. Журнал акушерства и женских болезней. 2013; 62(2): 63–74.
19. Горшинова В.К. Персонализация программы экстракорпорального оплодотворения у женщин с избыточной массой тела и ожирением на основании оценки функциональной активности митохондриального аппарата: дисс. … канд. мед. наук. М.; 2016.
20. Gilchrist R.B., Lane M., Thompson J.G. Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality. Hum. Reprod. Update. 2008; 14(2): 159–77.
21. Huang Z., Wells D. The human oocyte and cumulus cells relationship: new insights from the cumulus cell transcriptome. Mol. Hum. Reprod. 2010; 16(10): 715–25.
22. Grindler N.M., Moley K.H. Maternal obesity, infertility and mitochondrial dysfunction: potential mechanisms emerging from mouse model systems. Mol. Hum. Reprod. 2013; 19(8): 486–94.
23. Kattal N., Cohen J., Barmat L.I. Role of coculture in human in vitro fertilization: a meta-analysis. Fertil. Steril. 2008; 90(4): 1069–76.
24. Parikh F.R., Nadkarni S.G., Naik N.J., Naik D.J., Uttamchandani S.A. Cumulus coculture and cumulus-aided embryo transfer increases pregnancy rates in patients undergoing in vitro fertilization. Fertil. Steril. 2006; 86(4): 839–47.
25. Lin Y.H., Hwang J.L., Seow K.M., Huang L.W., Chen H.J., Tzeng C.R. Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes. Reprod. Biomed. Online. 2009; 19(2): 165–70.
26. Bavister B.D. Culture of preimplantation embryos: facts and artifacts. Hum. Reprod. Update. 1995; 1(2): 91–148.
27. Bongso A., Ng S.C., Fong C.Y., Anandakumar C., Marshall B., Edirisinghe R., Ratnam S. Improved pregnancy rate after transfer of embryos grown in human fallopian tubal cell co-culture. Fertil. Steril. 1992; 58(3): 569–74.
28. Jayot S., Parneix I., Verdaguer S., Discamps G., Audebert A., Emperaire J.C. Coculture of embryos on homologous endometrial cells in patients with repeated failures of implantation. Fertil. Steril. 1995; 63(1): 109–14.
29. Mansour R.T., Aboulghar M.A., Serour G.I., Abbass A.M. Co-culture of human pronucleate oocytes with their cumulus cells. Hum. Reprod. 1994; 9(9):1727–9.
30. Quinn P., Margalit R. Beneficial effects of coculture with cumulus cells on blastocyst formation in a prospective trial with supernumerary human embryos. J. Assist. Reprod. Genet. 1996; 13(1): 9–14.
31. Johnson J., Higdon H., Boone W. Effect of cumulus cell coculture using standard culture media on the maturation and fertilization potential of immature human oocyte. Fertil. Steril. 2008; 90(5): 1674–9.
32. Saito H., Hirayama T., Koike K., Saito T., Nohara M., Hiroi M. Cumulus mass maintains embryo quality. Fertil. Steril. 1994; 62(3): 555–8.
33. Benkhalifa M., Demirol A., Sari T., Balashova E., Tsouroupaki M., Giakoumakis Y., Gurgan T. Autologous embryo-cumulus cells co-culture and blastocyst transfer in repeated implantation failures: a collaborative prospective randomized study. Zygote. 2012; 20(2): 173–80.
34. Karakaya C., Guzeloglu-Kayisli O., Uyar A., Kallen A.N., Babayev E., Bozkurt N. et al. Poor ovarian response in women undergoing in vitro fertilization is associated with altered microRNA expression in cumulus cells. Fertil. Steril. 2015; 103(6): 1469–76. e1–3.
35. Lee K.S., Joo B.S., Na Y.J., Yoon M.S., Choi O.H., Kim W.W. Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET. J. Assist. Reprod. Genet. 2001; 18(9): 490–8.
36. Vithoulkas A. et al. Co-culture of human embryos with autologous cumulus cell clusters and its beneficial impact of secreted growth factors on preimplantation development as compared to standard embryo culture in assisted reproductive technologies (ART). Middle East Fertil. Soc. J. 2017; 22(4): 317–22.
37. McKenzie L.J., Pangas S.A., Carson S.A., Kovanci E., Cisneros P., Buster J.E. et al. Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF. Hum. Reprod. 2004; 19(12): 2869–74.
38. Feuerstein P., Cadoret V., Dalbies-Tran R., Guerif F., Bidault R., Royere D. Gene expression in human cumulus cells: one approach to oocyte competence. Hum. Reprod. 2007; 22(12): 3069–77.
39. Van Montfoort A.P., Geraedts J.P., Dumoulin J.C., Stassen A.P., Evers J.L., Ayoubi T.A. Differential gene expression in cumulus cells as a prognostic indicator of embryo viability: a microarray analysis. Mol. Hum. Reprod. 2008; 14(3): 157–68.
40. Ekart J., McNatty K., Hutton J., Pitman J. Ranking and selection of MII oocytes in human ICSI cycles using gene expression levels from associated cumulus cells. Hum. Reprod. 2013; 28(11): 2930–42.
Received 06.02.2018
Accepted 02.03.2018
About the Authors
Asfarova, Gyunay R., Post–graduate student, Department of assistive technologies in infertility treatment Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Healthcare of the Russian Federation Academica Oparina st. 4, 117997, Moscow, Russia. Tel. +7–926–262–11–13, e–mail: asfarovag@gmail.comSmolnikova, Veronica Yu., PhD, Leading researcher, Department of assistive technologies in infertility treatment Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Healthcare of the Russian Federation Academica Oparina st. 4, 117997, Moscow, Russia. e–mail: veronika.smolnikova@mail.ru
Makarova, Natalia P., PhD, Researcher of IVF Department
Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Healthcare of the Russian Federation Academica Oparina st. 4,
117997, Moscow, Russia. Tel. +7(495) 438–77–00 E–mail: np_makarova@oparina4.ru
Bobrov, Mikhail Yu., MD, Head of pathomorphology research group
Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Healthcare of the Russian Federation Academica
Oparina st. 4, 117997, Moscow, Russia. e–mail: m_bobrov@oparina4.ru
Kalinina, Elena A., MD, The chief of department of assisted reproductive technology in treating sterility
Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Healthcare of the Russian Federation Academica
Oparina st. 4, 117997, Moscow, Russia. Tel.+7(495)438–13–41 E–mail: e_kalinina@oparina4.ru
Romanov, Evgeniy A., clinical embryologist of department of assisted reproductive technology in treating sterility Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Healthcare of the Russian Federation Academica Oparina st. 4, 117997, Moscow, Russia.
E–mail: e_romanov@oparina4.ru
For citations: Brodsky G.V., Adamyan L.V., Sukhikh G.T. Autologous embryo-cumulus cell co-culturing in ART programs. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (11): 10-4. (in Russian)
https://dx.doi.org/10.18565/aig.2018.11.10-14
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