Association of vitamin D receptor (VDR) gene polymorphism with embryological characteristics and effectiveness of in vitro fertilization programs

Naimi Z.M.S., Kalinina E.A., Donnikov A.E., Dudarova A.Kh.

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow 117997, Ac. Oparina str. 4, Russia
Objective. To study the significance of vitamin D receptor (VDR) gene polymorphism as a molecular genetic marker for estimating the rate of implantation and clinical pregnancy and the patterns of oogenesis and embryogenesis in assisted reproductive technology programs. Subjects and methods. A total of 142 patients who had undergone an in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) program with superovulation and embryo transfer in this cycle were examined during a prospective study case-control study. Serum 25-hydroxyvitamin D levels and VDR gene polymorphism were ascertained in all the patients. The women were divided into 3 groups according to the level of vitamin D; 1) blood vitamin D deficiency (<10 ng/ml); 2) blood vitamin D insufficiency (10-29 ng/ml); 3) normal blood vitamin D levels (30–100 ng/ml). Results. Analysis of the association of VDR gene polymorphism with serum vitamin D levels established that the patients with A/G and A/A genotypes of VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) [rs 10735810] had serum vitamin D deficiency in 13.4% of women, vitamin D insufficiency in 70.1%, and normal vitamin D levels in 16.5% of the women included in the study. At the same time, the patients with G/G genotype of VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) were found to have blood vitamin D deficiency in 40% and insufficiency of this vitamin in 60%. The findings suggest that the carriage of G/G genotype of this polymorphism more than doubles the risk of serum vitamin D deficiency. The analysis of the association of VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) with the quality of the embryos obtained from women with different serum vitamin D levels revealed that the carriage of G/G genotype of the polymorphism (compared to A/A and A/G genotypes) reduced the risk of having poor-quality embryos by more than 3 times. Conclusion. VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) [rs 10735810] is an independent factor that can predict the serum vitamin D level that affects the outcomes of assisted reproductive technology programs and embryo quality and can complement the arsenal of already existing markers.

Keywords

gene polymorphisms
molecular genetic markers
vitamin D
infertility
stimulated cycle
in vitro fertilization
vitamin D receptor

Supplementary Materials

  1. Table 1. Ноrmonal profile of patients under research
  2. Table 2. Embryological characteristics of patients under research
  3. Pic. 1. Assosiation between polymorphism of the VDR 2A>G (Lys2Arg) (FokI) gene and the level of vitamin D in blood serum (* - allele А or G)
  4. Pic. 2. Number of bad quality embryos (in %) depending on polymorphism of the VDR 2A>G (Lys2Arg) (FokI) gene and the level of vitamin D in blood serum (* - allele А or G)

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Received 03.11.2016

Accepted 11.11.2016

About the Authors

Naimi Zokhra Mokhamad Sami, graduate student of department of assisted reproductive technology in treating sterility, Research Center of Obstetrics,
Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382501. E-mail: naimi-zohra@mail.ru
Kalinina Elena Anatolievna, MD, The chief of department of assisted reproductive technology in treating sterility, Research Center of Obstetrics,
Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381341. E-mail: e_kalinina@oparina4.ru
Donnikov Andrew Evgenievich, PhD, Senior Researcher of molecul-genetical laboratory, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381341. E-mail: a_donnikov@oparina4.ru
Dudarova Alina Khasanovna, graduate student of department of assisted reproductive technology in treating sterility, Research Center of Obstetrics,
Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382501. E-mail: kanshaov85@mail.ru

For citations: Naimi Z.M.S., Kalinina E.A., Donnikov A.E., Dudarova A.Kh. Association of vitamin D receptor (VDR) gene polymorphism with embryological characteristics and effectiveness of in vitro fertilization programs. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (2): 51-7. (in Russian)
http://dx.doi.org/10.18565/aig.2017.2.51-7

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