Activated glycyrrhizic acid in the combination treatment of patients with vaginitis and dysbiosis:results of a multicenter study

Kachalina O.V., Matuzkova A.A.

Volga Research Medical University, Ministry of Health of Russia, Nizhny Novgorod, Russia
Objective: To study the effect of 0.1% activated glycyrrhizic acid spray on the relief of vulvar discomfort and pathological leucorrhoea in women with nonspecific (aerobic), vulvovaginal candidiasis and bacterial vaginosis.
Materials and methods: The study was conducted under the supervision of the principal investigator with the participation of outpatient healthcare facilities providing obstetric and gynecological care in the cities: Moscow, Saint Petersburg, Voronezh, Belgorod, Krasnodar, Rostov-on-Don, Stavropol, Yekaterinburg, Chelyabinsk, Ufa, Novosibirsk , Krasnoyarsk, Irkutsk, Samara, Saratov, Kazan, and Nizhny Novgorod. The investigation enrolled 8,249 patients aged 18 to 45 years who complained of pathological vaginal discharge, discomfort in the external genital area with a clinical diagnosis of nonspecific (aerobic) vaginitis, vulvovaginal candidiasis, or bacterial vaginosis. All the patients underwent a comprehensive examination, including a physical and gynecological examination, bacterioscopy of vaginal smears, and comprehensive quantitative assessment of the vaginal microflora using a real-time polymerase chain reaction assay. All the patients were prescribed etiotropic therapy depending on the pathogen and clinical diagnosis at the discretion of an attending physician, by adding 0.1% activated glycyrrhizic acid (Epigen Intim) spray.
Results: The combination therapy resulted in a reduction of discomfort symptoms, such as itching (from 1.45 to 0.35 scores), burning (from 1.44 to 0.35 scores), unpleasant odor (from 1.31 to 0.51 scores), profuse discharge (from 1.8 to 0.30 scores), vulvar and vaginal mucosal dryness (from 0.44 to 0.16 scores), painful urination (from 0.37 to 0.14 scores), and dyspareunia (from 0.71 to 0.16 scores). The nature of discharge and its volume were stated to normalize (the proportion of patients with an abnormal discharge volume decreased from 3,812/8,249 (46.6%) to 101/8,249 (1.1%)).
Conclusion: When included in the combination treatment of patients with clinical manifestations of aerobic vaginitis, vulvovaginal candidiasis, and bacterial vaginosis, the drug based on 0.1% activated glycyrrhizic acid spray for local and topical application was found to relieve (cease) complaints of pathological vaginal discharge and vulvovaginal discomfort and to be safe and well tolerated.

Authors' contributions: Kachalina O.V. – development of the design of the study, material collection and processing, writing the article; Matuzkova A.A. – participation in the compilation of a literary review, work with the sources.
Conflicts of interest: The authors declare that there are no conflicts of interest.
Funding: The investigation has not been sponsored.
Acknowledgements: The authors are grateful to the research participants: Rosyuk E.A. (Yekaterinburg), Smolyanko I.V. (Samara), Zavodskaya O.N. (Samara), Skidan T.N. (Moscow), Skvortsova M.A. (Saratov), Aleshina E.A. (Moscow), Berger A.L. (Moscow), Gerasimova (Nelepova) Yu. V. (Moscow), Kharitonova Z.M. (Moscow), Gitinova Kh.Kh. (Moscow), Prismakova N.G. (Moscow), Otyutskaya A.V. (Moscow), Berestyanskaya I.B. (Moscow), Smagina E.N. (Moscow), Goyaeva R.Yu. (Moscow), Samarina E.A. (Moscow), Azimova D.M. (Ufa), Petrosyan P.A. (Moscow), Kolomytsina N.V. (Moscow), Aboneeva N.G. (Stavropol), Belousova V.A. (Stavropol), Dikanskaya K.V. (Stavropol), Nazhmudinova S.Z. (Moscow), Spiridonova O.A. (Moscow), Lipanina T.S. (Moscow), Sheshegova E.N. (Moscow), Feoktistova T.Yu. (Krasnoyarsk), Borisova T.P. (Krasnoyarsk), Kovalenko I.A. (Novosibirsk), Alibekova N.A. (Moscow), Zavodskaya O.N. (Samara), Neverova L.E. (Novosibirsk), Kosolapova E.V. (Kazan), Burnasheva F.A. (Kazan), Perminova E.I. (Novosibirsk), Semakina I.V. (Yekaterinburg).
Ethical Approval: The investigation has been approved by the Ethics Committee «BioEthic».
Patient Consent for Publication: The patients have signed an informed consent form to the publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Kachalina O.V., Matuzkova A.A.
Activated glycyrrhizic acid in the combination treatment of patients
with vaginitis and dysbiosis:results of a multicenter study.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2023; (3): 115-120 (in Russian)


nonspecific vulvovaginitis
vulvovaginal candidiasis
bacterial vaginosis
pathological discharge
vulvovaginal discomfort
activated glycyrrhizic acid


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Received 31.01.2023

Accepted 16.03.2023

About the Authors

Olga V. Kachalina, Professor, Department of Obstetrics and Gynecology, FDPE, Volga Research Medical University, Ministry of Health of Russia,,
603005, Russia, Nizhny Novgorod, Minin and Pozharsky sqr., 10/1.
Alina A. Matuzkova, student, Volga Research Medical University, Ministry of Health of Russia,, 603005, Russia, Nizhny Novgorod,
Minin and Pozharsky sqr., 10/1.

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