The level of VEGFA mRNA as a marker for chronic endometritis during the window of implantation
Objective. To elaborate diagnostic criteria for chronic endometritis by the mRNA expression profile in the endometrium during the implantation window.Tabolova V.K., Korneeva I.E., Donnikov A.E., Burmenskaya O.V.
Subjects and methods. Thirty-three patients with tuboperitoneal infertility were examined and divided into 2 groups: 1) 15 women, whose histological examination of endometrial scrapings in the proliferation phase revealed plasma cells; 2) 18 women, in whom no plasma cells were detected. The endometrial mRNA expression for 39 genes (markers for growth factors, cytokines, surface markers for immune system cells, matrix metalloproteinases, homeobox genes, transcription factors, estrogen and progesterone receptors) during the window of implantation was analyzed.
Results. The mid-secretory phase expression of VEGFA mRNA with a sensitivity of 64% and a specificity of 89% was associated with the presence of plasmocytes in Phase 1 of the menstrual cycle. The mid-secretory phase endometrium in the presence of plasmocytes showed increases in VEGFA mRNA by 1.7 times, VEGFA189 by 2.4 times, IGFBP1 by 16.6%, and CD68 by 2 times and also a 1.5-fold reduction in IL-2 mRNA expression compared with the patients having no plasmocytes.
Conclusion. The findings allowed the authors to elaborate diagnostic criteria for chronic endometritis in the mid-secretory phase by the VEGFA mRNA level during the window of implantation.
Keywords
chronic endometritis
infertility
endometrial pathology
gene expression
VEGFA
diagnosis
Supplementary Materials
- Table 1. Comparison of the results characteristic of endometrial biopsies histology in phase 1 and 2 of the menstrual cycle (n = 33)
- Table 2. Comparative characteristic of mRNA gene expression in endometrial biopsies in phase of medium secretion (n = 33)
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Received 08.02.2017
Accepted 17.02.2017
About the Authors
Tabolova V.K., graduate students Department of Fertility Preservation and Restoration, Research Centre of Obstetrics, Gynaecology and Perinatology,Minostry of Health of Russia. 117997, Russia, Moscow, Oparin str. 4. Tel.: +79188275828. E-mail: v.tabolova@mail.ru
Korneeva I.E., MD, PhD, senior researcher, Department of Fertility Preservation and Restoration, Research Centre of Obstetrics, Gynaecology and Perinatology,
Minostry of Health of Russia. 117997, Russia, Moscow, Oparin str. 4. Tel.: +79035939748. E-mail: irina.korneeva@inbox.ru
Donnikov A.E., Ph. D. scientist of the laboratory of molecular genetics methods, Research Centre of Obstetrics, Gynaecology and Perinatology, Minostry of Health of Russia. 117997, Russia, Moscow, Oparin str. 4. Tel.: +74954381183. E-mail: donnikov@dna-technology.ru
Bourmenskaya O.V., Ph.D. scientist of the laboratory of molecular genetics methods, Research Centre of Obstetrics, Gynaecology and Perinatology,
Minostry of Health of Russia. 117997, Russia, Moscow, Oparin str. 4. Tel.: +79167531709. E-mail: bourmenska@mail.ru
For citations: Tabolova V.K., Korneeva I.E., Donnikov A.E., Burmenskaya O.V. The level of VEGFA mRNA as a marker for chronic endometritis during the window of implantation. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (10): 62-8. (in Russian)
https://dx.doi.org/10.18565/aig.2017.10.62-68
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