The expression level of long non-coding RNAs ROR and MALAT1 in endometriosis
Objective. To estimate the expression level of long non-coding RNAs ROR and MALAT1 in the endometriosis-affected ovarian tissue biopsy specimens relative to the control group.Levakov S.A., Mamedova A.E., Azadova G.Ya., Paukov S.V., Maslyakova M.I., Pavlyukov M.S., Shakhparonov M.I., Antipova N.V.
Materials and methods. The endometrioid ovarian cyst and endometriosis-unaffected ovarian tissue samples from 20 reproductive-aged women were examined. The expression of long non-coding RNAs ROR and MALAT1 was analyzed by real-time PCR using primers that were specific to these genes.
Resu1ts. There was a substantial decrease in the expression level of long non-coding RNA ROR and an increase in the expression of MALAT1 in the endometriosis-affected tissue samples relative to the control group.
Conclusion. The investigation revealed a relationship between the decreased level of lncROR expression, the increased level of MALAT1, and the presence of ovarian endometriosis. The findings suggest that long non-coding RNAs play a possible role in the development of proliferative processes in ovarian endometriosis and correlate with the data obtained from the analysis of uterine myoma samples. Further investigations may determine the clinical potential of long non-coding RNAs in the diagnosis and treatment of proliferative diseases of the female reproductive system, in particular precancerous ovarian lesions.
Keywords
endometriosis
long non-coding RNAs
lncROR
MALAT1
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Received 29.12.2020
Accepted 02.04.2021
About the Authors
Sergey A. Levakov, MD, Professor, Head of the Department of Obstetrics and Gynecology, Institute of Clinical Medicine named after N.V. Sklifosovsky, I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University). Tel.: +7(925)506-31-44. E-mail: levakoff@yandex.ru. ORCID: 0000-0002-4591-838X.119991, Russia, Moscow, Trubetskaya str., 8-2.
Ainur E. Mamedova, postgraduate student of the Department of Obstetrics and Gynecology, Institute of Clinical Medicine named after N.V. Sklifosovskiy, I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University). Tel.: +7(968)400-73-02. E-mail: venesuela93@gmail.com. 0000-0002-9642-4523.
119991, Russia, Moscow, Trubetskaya str., 8-2.
Gyullar Ya. Azadova, postgraduate student of the Department of Obstetrics and Gynecology, Institute of Clinical Medicine named after N.V. Sklifosovsky, I.M. Sechenov
First Moscow State Medical University, Ministry of Health of Russia (Sechenov University). Tel.: +7(985)083-18-08. E-mail: gullyar.azadova@mail.ru.
119991, Russia, Moscow, Trubetskaya str., 8-2.
Sergey V. Paukov, Ph.D., Associate Professor of the Department of Obstetrics and Gynecology, Institute of Clinical Medicine named after N.V. Sklifosovsky, I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University). Tel.: +7(985)411-11-66. E-mail: spaukov@mail.ru.
119991, Russia, Moscow, Trubetskaya str., 8-2.
Marina I. Masliakova, student, National Research University «Higher School of Economics». E-mail: marimasmari@gmail.com.
101000, Russia, Moscow, Myasnitskaya str., 20.
Marat S. Pavlyukov, Doctor of Biological Sciences, Senior Researcher of the Laboratory of Membrane and Bioenergetic Systems, Academicians M.M. Shemyakin and
Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences. E-mail: marat.pav@mail.com. 117997, Russia, Moscow, Miklukho-Maklay str., 16/10.
Mikhail I. Shakhparonov, Doctor of Chemistry, Leading Researcher of the Laboratory of Membrane and Bioenergetic Systems, Academicians M.M. Shemyakin and
Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences. E-mail: shakhparonov@gmail.com. ORCID: 0000-0001-5965-8067.
117997, Russia, Moscow, Miklukho-Maklay str., 16/10.
Nadezhda V. Antipova, Ph.D., Senior Researcher of the Laboratory of Membrane and Bioenergetic Systems, Academicians M.M. Shemyakin and Yu.A. Ovchinnikov
Institute of Bioorganic Chemistry, Russian Academy of Sciences. Tel.: +7(977)773-22-07. E-mail: Nadine.antipova@gmail.com. ORCID: 0000-0002-5799-7767.
117997, Russia, Moscow, Miklukho-Maklay str., 16/10
For citation: Levakov S.A., Mamedova A.E., Azadova G.Ya., Paukov S.V., Maslyakova M.I., Pavlyukov M.S., Shakhparonov M.I., Antipova N.V. The expression level of long non-coding RNAs ROR and MALAT1 in endometriosis.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2021; 5: 141-145 (in Russian)
https://dx.doi.org/10.18565/aig.2021.5.141-145
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