The role of gene polymorphisms of the hemostatic system and folate cycle enzymes in the development of retrochorial hematoma
Objective. To assess the role of gene polymorphisms of the hemostatic system and folate cycle enzymes in the development of retrochorial hematoma (RCH).Kuznetsova N.B., Bushtyreva I.O., Donnikov A.E., Mashkina E.V., Dybova V.S.
Subjects and methods. The investigation enrolled 305 pregnant women who were examined and treated at the Rostov Regional Perinatal Center. All the pregnant women were divided into 2 groups: 1) 238 pregnant women with RCH; 2) (a control group) 67 pregnant women with clinically normal pregnancy without RCH, as evidenced by first-trimester ultrasound. To identify genetic markers determining the development of chorion detachment, the pregnant women in the first-trimester underwent genotyping of four polymorphic loci of the folate cycle genes (MTHFR C677T, MTHFR A1298C, MTR А2756G, and MTRR А66G) and eight hemostatic genes (F2 G20210A, F5 G1691A, G10976A F7, F13 G103T, FGB G-455А, C807T ITGA2, ITGB3 Т1565С, and SERPINE1-675 5G/4G).
Results. Significant differences were obtained in a study of the distribution of the alleles G10976A
of the F7 gene, G103T of the F13 gene, and G-455А of the FGB gene in a group of pregnant women with RCH versus that of pregnant women with clinically normal pregnancy. The A allele of F7 G10976A, the T allele
F13 G103T, and the A allele of FGB G-455A were found to be associated with a high risk for RCH.
Conclusion. The F7 gene (proconvertin, CF VII) polymorphism G10976A (rs 6046), the F13 gene (Fibrinase, CF XIII) G103T polymorphism (rs 5985), and the FGB gene (fibrinogen β-chain) polymorphism G-455А (rs 1800790) are associated with the risk of RCH.
Keywords
retrochorial hematoma
gene polymorphisms
folate cycle genes
hemostatic genes
Supplementary Materials
- Table 1. Frequencies of genotypes and alleles of a polymorphic gene of F7 G10976A (rs 6046) in pregnant women with a retrochorial hematoma in the I trimester and in group of control.
- Table 2. Frequencies of genotypes and alleles of a polymorphic gene of F13 G103T (rs 5985) in pregnant women with a retrochorial hematoma in the I trimester and in group of control.
- Table 3. Frequencies of genotypes and alleles of a polymorphic gene of FGB G-455A (rs 1800790) in pregnant women with retrochorial hematoma in the I trimester and in group of control.
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Received 16.12.2016
Accepted 23.12.2016
About the Authors
Kuznetsova Natalia Borisovna, Ph.D. in medical sciences, the assistant of the Department of Obstetrics, Gynecology and Reproduction № 4, Rostov State Medical University, Ministry of Health of Russia. 344022, Russia, Rostov-on-Don, Nakhichevan lane 29. Tel.: +79287709762. E-mail: lauranb@inbox.ruBushtyreva Irina Olegovna, MD, Professor, the Head of the Department of Obstetrics, Gynecology and Reproduction № 4, Rostov State Medical University,
Ministry of Health of Russia; leading researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
344022, Russia, Rostov-on-Don, Nakhichevan lane 29. Tel.: +7928770972. E-mail: kio4@mail.ru
Donnikov Andrey Evgenievich, Ph.D. in medical sciences, leading researcher of Laboratory of molecular genetic methods, Research Center of Obstetrics,
Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382088. E-mail: a_donnikov@oparina4.ru
Mashkina Elena Vladimirovna, Ph.D. in biological sciences, the assistant of the genetics department of Federal Autonomous Educational Institution of Higher Education the Southern Federal University. 344006, Russia, Rostov-on-Don, Stachki avenue 194/1, office 319. Tel.: +7(863)219-87-94. E-mail: lenmash@mail.ru
Dybova Violetta Sergeevna, obstetrician-gynecologist, Perinatal Center, Rostov-on-Don.
344068, Russia, Rostov-on-Don, Bodraya str. 90. Tel.: +79612720412. E-mail: viola-kovaleva@mail.ru. http://orcid.org/0000-0002-2391-8952
For citations: Kuznetsova N.B., Bushtyreva I.O., Donnikov A.E.,
Mashkina E.V., Dybova V.S. The role of gene polymorphisms of the hemostatic system and folate cycle enzymes in the development of retrochorial hematoma.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (3): 62-7. (in Russian)
http://dx.doi.org/10.18565/aig.2017.3.62-7
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